A5102751332- Immune Cell Function and Interaction
- Connexins and lens biology
- Mesenchymal stem cell research
- Genetics and Neurodevelopmental Disorders
- Mitochondrial Function and Pathology
- T-cell and B-cell Immunology
- Metabolism and Genetic Disorders
- Peroxisome Proliferator-Activated Receptors
- Autism Spectrum Disorder Research
- Metastasis and carcinoma case studies
- CAR-T cell therapy research
- Ion Channels and Receptors
- Cancer, Hypoxia, and Metabolism
- Folate and B Vitamins Research
- Pluripotent Stem Cells Research
- Inflammatory mediators and NSAID effects
- Colorectal and Anal Carcinomas
- Down syndrome and intellectual disability research
- Immunotherapy and Immune Responses
- Chemokine receptors and signaling
- Bone and Joint Diseases
- Ginseng Biological Effects and Applications
- Burn Injury Management and Outcomes
- Lung Cancer Research Studies
- Allergic Rhinitis and Sensitization
Nagasaki International University
2024
Kyushu University
1999-2023
Tokyo Women's Medical University
2013-2016
Niigata University of Health and Welfare
2016
Matsumoto Meat Hygienic Inspection Center of Nagano Prefecture
2005
Chiba University
1996-2003
Shimane University
2000-2002
University of Yamanashi
2002
Kirin (Japan)
1997-1998
Japan Science and Technology Agency
1997
Natural killer T (NKT) lymphocytes express an invariant cell antigen receptor (TCR) encoded by the Valpha14 and Jalpha281 gene segments. A glycosylceramide-containing alpha-anomeric sugar with a longer fatty acyl chain (C26) sphingosine base (C18) was identified as ligand for this TCR. Glycosylceramide-mediated proliferative responses of NKT cells were abrogated treatment chloroquine-concanamycin or monoclonal antibodies against CD1d/Vbeta8, CD40/CD40L, B7/CTLA-4/CD28, but not interference...
A lymphocyte subpopulation, the V α 14 natural killer T (NKT) cells, expresses both NK1.1 and a single invariant cell receptor encoded by J 281 gene segments. Mice with deletion of segment were found to exclusively lack this subpopulation. The NKT cell–deficient mice could no longer mediate interleukin-12 (IL-12)–induced rejection tumors. Although antitumor effect IL-12 was thought be mediated through cells an essential target IL-12, they their cytotoxicity NK-like effector mechanism after...
We have recently identified alpha-galactosylceramide (alpha-GalCer) as a specific ligand for an invariant Valpha14/Vbeta8.2 T cell receptor exclusively expressed on the majority of Valpha14 NKT cells, novel subset lymphocytes. Here, we report that alpha-GalCer selectively activates cells resulting in prevention tumor metastasis. The effector mechanisms ligand-activated seem to be mediated by natural killer (NK)-like nonspecific cytotoxicity. Indeed, cytotoxic index obtained...
Peroxisome proliferator-activated receptor (PPAR) gamma is expressed in human colon cancer, prostate cancer and breast cells, PPARgamma activation induces growth inhibition these cells. expression gastric however, has not been fully investigated. We report the effect of ligands on proliferation carcinoma cell lines. Immunohistochemistry was used to demonstrate presence protein surgically resected specimens from well differentiated, moderately differentiated poorly adenocarcinoma. reverse...
NK1.1+ T [natural killer (NK) T] cells express an invariant cell antigen receptor alpha chain (TCR alpha) encoded by V 14 and J 281 segments in association with a limited number of betas, predominantly beta 8.2. Expression the 14/J 281, but not 1, TCR transgenic mice lacking endogenous expression blocks development conventional leads to preferential NK cells, suggesting prerequisite role development. In 8.2 B 3 mice, two different CD3 epsilon expressions, epsilon(dim) epsilon(high), can be...
Peroxisome proliferator-activated receptor γ (PPARγ) ligands have been implicated in the growth inhibition and differentiation of certain human cancers with diverse tissue origin. In this study, expression PPARγ hepatocellular carcinoma (HCC) effect on HCC cells were investigated vitro using Hep G2, HuH-7, KYN-1 KYN-2 cell lines. All lines found to express functionally active a marked was induced by thiazolidinedione troglitazone, pioglitazone as well its natural ligand...
Reg is a growth factor with mitogenic effects on pancreatic beta cells and gastric stem cells. To date, there has been no information available Reg-mediated intracellular signal transduction pathways. The role of in the carcinogenesis also unknown. In current study, signaling pathway cancer cell was examined. treatment MKN45 resulted tyrosyl-phoshorylation several cellular proteins subsequent activation classical MAPK, ERK1/2. stimulated thymidine incorporation AGS dose-dependent manner....
Autism spectrum disorder (ASD) is a highly heterogeneous neurodevelopmental characterized by impaired social interactions, restrictive interests, and repetitive stereotypic behaviors. Among the various mechanisms underlying pathogenesis of ASD, dysfunctions dopaminergic signaling mitochondria have been hypothesized to explain core symptoms children with ASD. However, only few studies focusing on pathological association between neurons (DN) in ASD performed using patient-derived stem cells...
Vα14 NKT cells express an invariant antigen receptor encoded by and Jα281 gene segments as well natural killer (NK) markers, including NK1.1. Here, we describe a precursor population of (pre-NKT) that expresses NK1.1, T cell β, pTα, RAG1/2 but not surface CD3ɛ. Such pre-NKT were differentiated successfully in vitro into mature CD3ɛ + IL-15 granulocyte/macrophage colony-stimulating factor (GM-CSF) conjunction with stroma cells. Interestingly, only GM-CSF without induced the Vα14-Jα281...
Metatropic dysplasia is a congenital skeletal characterized by severe platyspondyly, dumbbell-like deformity of long tubular bones, and progressive kyphoscoliosis with growth. It caused mutations in the gene TRPV4, encoding transient receptor potential vanilloid 4, which acts as calcium channel. Many heterozygous single base this have been associated disorder, showing autosomal dominant inheritance. Although abnormal endochondral ossification has observed histological examination bone...
Stem cells from human exfoliated deciduous teeth (SHED) are isolated the dental pulp tissue of primary and can differentiate into neuronal cells. Although SHED a desirable type stem for transplantation therapy study neurological diseases, large part differentiation machinery remains unclear. Recent studies have suggested that mitochondrial activity is involved in In present work, we investigated by focusing on activity. During SHED, observed increased membrane potential, DNA, elongated...
Contribution of immunosuppressive cytokines to tumor progression in many types cancers has been suggested. To characterize the vivo expression gastric cancer, we analyzed messenger RNA (mRNA) transforming growth factor-beta (TGF-beta) and interleukin-10 (IL-10) human carcinoma tissues.Both tissues nontumor from each resected specimen 29 primary carcinomas were tested for IL-10 TGF-beta mRNA by reverse transcriptase-polymerase chain reaction (RT-PCR), was correlated with various pathological...
Mitochondrial fission factor (MFF) is an adapter that targets dynamin-related protein 1 from the cytosol to mitochondria for fission. Loss-of-function MFF mutations cause encephalopathy due defective mitochondrial and peroxisomal 2 (EMPF2). To elucidate molecular mechanisms were involved, we analyzed functional effects of depletion in deciduous teeth-derived dental pulp stem cells differentiating into dopaminergic neurons (DNs). When treated with MFF-targeting small interfering RNA, DNs...
Mutations in the gene encoding transient receptor potential vanilloid member 4 (TRPV4), a Ca
Embryonic trisomy leads to abortion or congenital genetic disorders in humans. The most common autosomal chromosome abnormalities are of chromosomes 13, 18, and 21. Although alteration gene dosage is thought contribute caused by extra copies chromosomes, genes associated with specific disease phenotypes remain unclear. To generate a normal cell from trisomic as means etiological analysis candidate therapy for syndromes, we developed system eliminate targeted human cells. Chromosome 21 was...