A5030871949- Acute Lymphoblastic Leukemia research
- SARS-CoV-2 and COVID-19 Research
- Acute Myeloid Leukemia Research
- Immune Cell Function and Interaction
- Chronic Myeloid Leukemia Treatments
- SARS-CoV-2 detection and testing
- Epigenetics and DNA Methylation
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Cancer Genomics and Diagnostics
- Heparin-Induced Thrombocytopenia and Thrombosis
- Childhood Cancer Survivors' Quality of Life
- Hematopoietic Stem Cell Transplantation
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Genomics and Rare Diseases
- Venous Thromboembolism Diagnosis and Management
- MicroRNA in disease regulation
- Immune cells in cancer
- Retinal and Optic Conditions
- Adolescent and Pediatric Healthcare
- RNA modifications and cancer
- Immunotherapy and Immune Responses
- Digital Imaging for Blood Diseases
- Platelet Disorders and Treatments
Goethe University Frankfurt
2021-2025
University Hospital Frankfurt
2023-2024
Cardio-Pulmonary Institute
2024
Deutschen Konsortium für Translationale Krebsforschung
2024
Frankfurt Cancer Institute
2024
To fight the COVID-19 pandemic caused by RNA virus SARS-CoV-2, a global vaccination campaign is in progress to achieve immunization of billions people mainly with adenoviral vector- or mRNA-based vaccines, all which encode SARS-CoV-2 Spike protein. In some rare cases, cerebral venous sinus thromboses (CVST) have been reported as severe side effect occurring 4-14 days after first and were often accompanied thrombocytopenia. Besides CVST, splanchnic vein (SVT) other thromboembolic events...
The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of disease COVID-19, which has become a global concern due to its rapid spread. common methods monitor and quantitate SARS-CoV-2 infectivity in cell culture are so far time-consuming labor-intensive. Using Sleeping Beauty transposase system, we generated robust versatile cellular infection model that allows experiments compatible for high-throughput live imaging. based on lung derived A549 cells, show...
Abstract Uncovering early gene network changes of human hematopoietic stem cells (HSCs) leading to differentiation induction is utmost importance for therapeutic manipulation. We employed single cell proteo-transcriptomic sequencing FACS-enriched bone marrow and progenitor (HSPCs) from 15 healthy donors. Pseudotime analysis reveals four major trajectories, which remain consistent upon aging, with an branching point into megakaryocyte-erythroid progenitors. However, young donors suggest a...
We introduce a novel approach for the control of oligonucleotides through enzymatic activation with β-galactosidase (β-gal). use well-known capability β-gal to hydrolyze β-galactosidic bond combined self-immolative linker and present three ways sterical or topological blocking DNA oligonucleotide. Through series in vitro experiments utilizing variants (recombinant from human cell lysates), we systematically investigate stability, transitory perturbation activation. Our holds significant...
The most frequent genetic aberration leading to infant ALL (iALL) is the chromosomal translocation t(4;11), generating fusion oncogenes KMT2A:AFF1 and AFF1:KMT2A, respectively. KMT2A-r iALL displays a dismal prognosis through high relapse rates relapse-associated mortality. Relapse occurs frequently despite ongoing chemotherapy without accumulation of secondary mutations. A rational explanation for observed chemo-resistance satisfactory treatment options remain be elucidated. We found that...
Abstract KMT2A -rearranged acute lymphoblastic infant leukemia ( -r iALL) is associated with outsize risk of relapse and mortality. We previously reported strong upregulation the immediate early gene EGR3 in :: AFF1 iALL at relapse; now we provide analyses regulome, which assessed through binding expression target analysis an EGR3-overexpressing t(4;11) cell culture model. Our data identify as a regulator B-lineage commitment. Principal component 50 patients diagnosis 18 provided strictly...
Summary The severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) is the causative agent of disease COVID-19, which has become a global concern due to its rapid spread. common methods monitor and quantitate SARS-CoV-2 infectivity in cell culture are so far time-consuming labor-intensive. Using Sleeping Beauty transposase system, we generated robust versatile reporter system that allows infection experiments compatible for high-throughput live imaging. based on lung derived A549 cells,...
Abstract IKZF1 deletions are associated with an increased risk of relapse in B-cell precursor acute lymphoblastic leukemia (B-ALL), and their accurate detection has great clinical impact. Here, we included four international cohorts pediatric adult patients B-ALL, reviewed literature to illustrate the recombination map deletions, a focus at non-recurrent deletions. We provide substantial basis for improvement diagnostic methods based on MLPA multiplex PCR identification also demonstrate that...
Background:IKZF1 deletions are associated with an increased risk of relapse in patients B-cell precursor acute lymphoblastic leukemia (B-ALL), and their accurate detection has great clinical impact. Aims: We illustrate the recombination map IKZF1 deletions, a focus on non-recurrent (neither Δ1-8, Δ2-3, Δ2-7, Δ2-8, Δ4-7, nor Δ4-8). In addition, we evaluate whether rare dismal prognosis for B-ALL patients, provide substantial basis improvement diagnostic methods based MLPA multiplex PCR...
The most frequent genetic aberration leading to infant ALL (iALL) is the chromosomal translocation t(4;11), generating fusion oncogenes KMT2A::AFF1 and AFF1::KMT2A. KMT2A-r iALL displays a dismal prognosis through high relapse rates relapse-associated mortality. Relapse occurs frequently despite ongoing chemotherapy without accumulation of secondary mutations. A rational explanation for observed chemo-resistance, satisfactory treatment options remain be elucidated. We found that elevated...
Infant proB ALL is mainly caused by the chromosomal translocation t(4;11), generating fusion oncogenes KMT2A::AFF1 and AFF1::KMT2A. Generally, KMT2A-rearranged iALL characterized dismal outcome due to high relapse incidences relapse-associated mor-tality. Relapse appears despite continuous chemotherapy but without selection of secondary mutations. Therefore, mechanism therapy failure formation remains be elucidated. Here, we present first evidence that cells overexpressing ICOSLG are...
The expansion of regulatory T cells (Tregs) is known to be mediated by cytokines including IL-10 and TGFβ but has additionally been shown depend on the interaction immune receptors ICOSLG ICOS. Here, we describe a co-culture system which enables quantification ability leukemia induce Treg through secreted direct receptor interactions. protocol applicable for MHC-matched -unmatched experiments allows assessment without using mouse model. For complete details use execution this protocol,...