A5046074731- Acute Myeloid Leukemia Research
- Acute Lymphoblastic Leukemia research
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Childhood Cancer Survivors' Quality of Life
- Chronic Myeloid Leukemia Treatments
- Multiple Sclerosis Research Studies
- Chemotherapy-related skin toxicity
- T-cell and B-cell Immunology
- Psoriasis: Treatment and Pathogenesis
- Fungal Infections and Studies
- Multiple Myeloma Research and Treatments
- Viral-associated cancers and disorders
- Single-cell and spatial transcriptomics
- CAR-T cell therapy research
- Cutaneous lymphoproliferative disorders research
- Hematopoietic Stem Cell Transplantation
- Nail Diseases and Treatments
- Immunodeficiency and Autoimmune Disorders
- Antifungal resistance and susceptibility
- Cancer Immunotherapy and Biomarkers
- Heat shock proteins research
Hospital Amaral Carvalho
2016-2025
Sabin Medicina Diagnostica (Brazil)
2020-2023
Instituto de Medicina Integral Professor Fernando Figueira
2023
Goethe University Frankfurt
2023
Weatherford College
2023
Keio University
1981-1989
Experimental autoimmune encephalomyelitis (EAE) is an animal model to study multiple sclerosis (MS). Considering the tolerogenic effects of active vitamin D, we evaluated therapeutic effect myelin oligodendrocyte glycoprotein (MOG) associated with D in EAE development. was induced female C57BL/6 mice by immunization MOG emulsified Complete Freund's Adjuvant plus Mycobacterium tuberculosis. Animals also received two intraperitoneal doses Bordetella pertussis toxin. One day after immunization,...
Chronic lymphocytic leukemia is characterized by clonal proliferation and progressive accumulation of B-cell lymphocytes that typically express CD19+, CD5+ CD23+. The usually infiltrate the bone marrow, peripheral blood, lymph nodes, spleen. diagnosis established immunophenotyping circulating B-lymphocytes, prognosis defined two staging systems (Rai Binet) physical examination blood counts, as well several biological genetic markers. In this update, we present recommendations from Brazilian...
Cryopreservation of spermatozoa is a pivotal tool in assisted reproduction, and studies aiming to establish optimal freezing/thawing protocols are essential enhance sperm survival. The objectives the present study were (1) compare cryoprotective efficiency three different glycerol concentrations (3%, 5%, 7%) on basis post-thaw quality (2) investigate whether incidence morphologically abnormal fresh samples related cryodamage sensitivity. Semen was collected from six tomcats using an...
Experimental autoimmune encephalomyelitis (EAE) is an artificially induced demyelination of the central nervous system (CNS) that resembles multiple sclerosis in its clinical, histopathological, and immunological features. Activated Th1 Th17 cells are thought to be main players during EAE development. This study was designed evaluate peripheral local contribution IL-17 acute chronic stages. C57BL/6 mice were immunized with MOG plus complete Freund’s adjuvant followed by pertussis toxin. Mice...
Paracoccidiodomycosis is a systemic mycosis caused by the fungus Paracoccidioides brasiliensis (Pb), which endemic in Latin America. The host innate immune response against has been well characterized and several studies have shown important role played phagocytic cells. Our laboratory studied relationship between human neutrophils (PMNs)/Pb, focusing effector mechanisms of these cells fungus. However, last years, that addition to their killer functions, PMNs can modulate instruct response,...
The objective of our study was to evaluate the effects zinc supplementation on cardiac remodeling following acute myocardial infarction in rats.Animals were subdivided into 4 groups and observed for 3 months: 1) Sham Control; 2) Zinc: animals receiving supplementation; 3) Infarction 4) Zinc. After followup period, we studied hypertrophy ventricular geometry, functional alterations vivo vitro, changes related collagen, oxidative stress, inflammation, assessed by echocardiogram, isolated heart...
Summary Type I diabetes is a disease caused by autoimmune destruction of the beta cells in pancreas that leads to deficiency insulin production. The aim this study was evaluate prophylactic potential prime-boost strategy involving bacille Calmette–Guérin (BCG) and pVAXhsp65 vaccine (BCG/DNAhsp65) induced streptozotocin (STZ) C57BL/6 mice also spontaneous type 1 non-obese diabetic (NOD) mice. BCG/DNAhsp65 vaccination NOD determined weight gain, protection against hyperglycaemia, decreased...
Minimal residual disease is the most powerful predictor of outcome in acute leukemia and useful therapeutic stratification for lymphoblastic protocols. Nowadays, reliable methods studying minimal are multiparametric flow cytometry polymerase chain reaction. Both provide similar results at a level 0.01% normal cells, that is, detection one leukemic cell up to 10,000 nucleated cells. Currently, protocols establish threshold value informative time points according appropriate methodology...
Background Actinic cheilitis ( AC ) is an oral potentially malignant lesion which the counterpart of actinic keratosis skin and has potential to develop into squamous cell carcinoma. Regulatory T cells (Tregs) have a critical role in modulating antitumor immune responses. The presence regulatory lesions not been described. We chose investigate involvement lesions. Methods frequency, phenotype, activity CD 4+ 25+ isolated from blood patients were analyzed by flow cytometry. Cytokines...
Abstract Background Bacterial superantigens are potent T cell activators that can activate cells with specificity for antigens of the central nervous system (CNS). In this study, we compared effect two S. aureus strains on experimental autoimmune encephalomyelitis (EAE) development. C57BL/6 female mice were infected ATCC 51650, which produces toxic shock syndrome toxin 1 (TSST-1+) or 43300, does not produce toxins (TOX-). Three days later, animals subjected to EAE induction by immunization...
The minimal residual disease (MRD) status plays a crucial role in the treatment of acute lymphoblastic leukemia (ALL) and is currently used most therapeutic protocols to guide appropriate decision. Therefore, it imperative that laboratories offer accurate reliable results through well standardized technical processes by establishing rigorous operating procedures. Our goal propose monoclonal antibody (MoAb) panel for MRD detection ALL provide recommendations intended flow cytometry work on...
Multiparametric flow cytometry (MFC) is a powerful tool for the diagnosis of hematological malignancies and has been useful classification chronic lymphoproliferative disorders (CLPD) according to WHO criteria. Following purposes Brazilian Group Flow Cytometry (GBCFLUX), aim this report was standardize minimum requirements achieve an accurate in CLPDs, considering different economic possibilities laboratories our country. Most Brazil work with 4-fluorescence cytometers, which why GBCFLUX...
Multiparameter flow cytometry (MFC) is a highly sensitive, fast and specific diagnostic technology with wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories equipped four-color cytometer facilities. Based on this fact, the Group Flow Cytometry (Grupo Brasileiro de Citometria Fluxo, GBCFLUX) for standardization has proposed an antibody panel designed to allow precise diagnosis...
Experimental autoimmune encephalomyelitis (EAE) is a demyelinating pathology of the central nervous system (CNS) used as model to study multiple sclerosis immunopathology. EAE has also been extensively employed evaluate potentially therapeutic schemes. Considering presence an immune response directed heat shock proteins (hsps) in diseases and immunoregulatory potential these molecules, we evaluated effect previous immunization with genetic vaccine containing mycobacterial hsp65 gene on...