A5065234371- Acute Myeloid Leukemia Research
- Lymphoma Diagnosis and Treatment
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Hematopoietic Stem Cell Transplantation
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Multiple Myeloma Research and Treatments
- Chronic Myeloid Leukemia Treatments
- Vascular Tumors and Angiosarcomas
- Hemoglobinopathies and Related Disorders
- Eosinophilic Disorders and Syndromes
- Blood disorders and treatments
- Single-cell and spatial transcriptomics
- Mast cells and histamine
- Viral-associated cancers and disorders
- Hematological disorders and diagnostics
- Brain Metastases and Treatment
- Retinoids in leukemia and cellular processes
- Platelet Disorders and Treatments
- Complement system in diseases
- CNS Lymphoma Diagnosis and Treatment
- Prenatal Screening and Diagnostics
- Immune cells in cancer
Hospital Israelita Albert Einstein
2015-2024
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2019
Hospital São Paulo
1999
To describe reference values for blood counts obtained from laboratory tests in the Brazilian adult population according to results National Health Survey (Pesquisa Nacional de Saúde - PNS), by gender, age group and skin color.The initial sample consisted of 8,952 adults. determine values, individuals with prior diseases outliers were excluded. Mean standard deviation limits stratified color.For red cells, men presented a mean value 5.0 million per mm3 (limits: 4.3-5.8) women, 4.5 3.9-5.1)....
Abstract Immunophenotyping by flow cytometry is an integral part of the diagnosis and classification leukemias/lymphomas. The expression ROR1 associated with chronic B lymphocytic leukemia (CLL) well described in literature, both its follow‐up minimal residual disease (MRD) research, however, there are few studies regarding pattern other subtypes mature lymphoid neoplasms. With aim evaluating associating it important markers for differentiation neoplasms (MBLN), 767 samples cases that...
To verify the prevalence of anemia in Brazilian adults and elderly.This is a cross-sectional study consisted 8,060 subjects aged over 18 years old all states. We used data from laboratory tests National Health Survey (Pesquisa Nacional de Saúde - PNS). The following indicators obtained by erythrogram were used: hemoglobin, mean corpuscular volume (MCV), hemoglobin (MCH), red cell distribution width (RDW). Reference values World Organization (WHO) to determine anemia, which considers levels...
Introduction: Infiltration of the central nervous system by hematologic or lymphoid malignant cells can cause extensive neurological damage, be progressive and fatal. However, usually, cerebrospinal fluid (CSF) has low cellularity rapid cell degeneration, which impair cytometry analysis. Storage transport measures, sample preparation staining protocols interfere with diagnostic accuracy. Objective: To calculate performance flow using a stabilizer for preservation compared to cytomorphology...
To describe the prevalence of hemoglobinopathies in Brazilian adult population, according to laboratory tests from National Health Survey.A descriptive study was carried out with Survey data collected between 2014 and 2015. The test performed using High Performance Liquid Chromatography method. results individual were interpreted as providing normal, homozygous or heterozygous for S, C D hemoglobin, addition other possible hemoglobinopathies. Prevalence gender, skin color, region, age...
Abstract Introduction Flow cytometry (FC) is a helpful tool for the diagnosis of myelodysplastic syndrome (MDS). Different FC score systems have been developed. The “Ogata score” simple diagnostic that has validated having sensitivity 69% and specificity 92% in low‐risk MDS. We aimed to study feasibility utility MDS among Latin America (LA) Laboratories. Methods This case control conducted LA institutions members Grupo Latinoamericano de Mielodisplasia (GLAM). A total 146 patients 57 were...
RESUMO: Introdução: Este artigo teve o objetivo de estimar valores referência exames laboratoriais colesterol, hemoglobina glicosilada e creatinina para a população adulta brasileira. Métodos: Estudo descritivo realizado com os dados da Pesquisa Nacional Saúde (PNS). Foram coletadas amostras sangue urina em subamostra PNS constituída 8.952 indivíduos 18 anos ou mais. Para determinar referência, aplicaram-se critérios exclusão, como presença doenças prévias dos outliers, definidos pelos fora...
Abstract Introduction The workflow in clinical flow cytometry laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity reduce costs. Using the Beckman Coulter dry coating technology, we customized a ten‐color tube with dried antibody reagents, designated Duraclone screening ( DST ), for hematological malignancies. Here, compared applicability, numerical equivalence, cost time required between liquid reagents . Methods contains CD 4 +...
Abstract Background Minimal residual disease (MRD) in chronic lymphocytic leukemia (CLL) has prognostic and predictive significance. One of the approaches to detect MRD by flow cytometry (FC) is use dry antibody reagents such as DuraClone® RE CLB (Beckman Coulter—BC). The aim this study was evaluate performance detecting CLL compared liquid reagents. Methods composed CD81FITC, ROR1PE, CD79bPC5.5, CD19PC7, CD5APC, CD43APCA750, CD20PB, CD45KrO. For reagent assay, we used CD43FITC, CD3ECD,...
Multiparameter flow cytometry is a highly sensitive, fast, and specific diagnostic technology with wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories equipped four-color cytometer facilities. Based on this fact, the Group Flow Cytometry (Grupo Brasileiro de Citometria Fluxo, GBCFLUX) for standardization has proposed an antibody panel designed to allow precise diagnosis...
Objective: To study the frequency of mutations that may lead to a good or bad prognosis, as well their relation with karyotype and immunophenotype in patients acute myeloid leukemia. Methods: Thirty samples leukemia were studied, which FLT3-ITD, FLT3-TKD NPM1 investigated. All submitted immunophenotyping 25 karyotyping. Results: An occurrence 33.3% mutation an equal number FLT3-ITD observed. When only cases normal this figures increased 50 40%, respectively. Eight percent genotype...
Multiparametric flow cytometry (MFC) is a powerful tool for the diagnosis of hematological malignancies and has been useful classification chronic lymphoproliferative disorders (CLPD) according to WHO criteria. Following purposes Brazilian Group Flow Cytometry (GBCFLUX), aim this report was standardize minimum requirements achieve an accurate in CLPDs, considering different economic possibilities laboratories our country. Most Brazil work with 4-fluorescence cytometers, which why GBCFLUX...
Myelodysplastic syndromes (MDS) are a heterogeneous group of hematopoietic stem cell diseases characterized by dysplasia one or more hematologic lineages and high risk developing into acute myeloid leukemia. MDS patients have recurrent bacterial infections abnormal expression CD56 monocytes. We investigated patients' bone marrow CD56+/CD56- monocytes their in vitro-derived dendritic populations comparison with cells obtained from disease-free subjects. found that patients, irrespective...
Multiparameter flow cytometry (MFC) is a highly sensitive, fast and specific diagnostic technology with wide range of applicability in hematology. Although well-established eight-color immunophenotyping panels are already available, most Brazilian clinical laboratories equipped four-color cytometer facilities. Based on this fact, the Group Flow Cytometry (Grupo Brasileiro de Citometria Fluxo, GBCFLUX) for standardization has proposed an antibody panel designed to allow precise diagnosis...
Cancer development results from the progressive accumulation of genomic abnormalities that culminate in neoplastic phenotype. Cytogenetic alterations, mutations and rearrangements may be considered as molecular legacy which trace clonal history disease. Concomitant tumors are reported they derive a common or divergent founder clone. B-cell chronic lymphocytic leukemia (B-CLL) plasma cell myeloma (PCM) both mature neoplasms, their concomitancy, albeit rare, is documented. Here, we described...
To identify antiplatelet antibodies by flow cytometry (direct method) in patients with thrombocytopenia.Between January 1997 and March 2004 a total of 15100 were referred to the Centro de Hematologia São Paulo for hematological investigation several diagnoses (anemia, leukopenia, thrombocytopenia, coagulation abnormalities, adenomegaly, leukemia others). Of those, 1057 because thrombocytopenia divided into two groups: Group Idiopathic thrombocytopenic purpura, no identifiable cause; Other...
A 71-year-old male who was immunosuppressed following liver transplantation underwent resection of a 3·1 mm-deep melanoma on his back. At the time surgery, positron emission tomography/computed tomography (PET-CT) scan and biopsy left sentinel axillary lymph nodes showed no evidence metastasis. Three months later he presented with back pain. Another PET-CT performed, which uptake in axilla diffuse activity bone marrow. Blood counts were normal lactic dehydrogenase 20 times normal. marrow...
The Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria Fluxo [GBCFLUX]), founded on April 24, 2010, is composed experts in flow cytometry (FC) area who have the common objective contributing to technical and scientific advances clinical research laboratories. Among GBCFLUX working groups, Quality Control (QC) subcommittee responsible for discussing data literature contributes quality assurance pre-analytical, analytical post-analytical process FC. QC subcommittee's actions...
To demonstrate the advantages of correlating flow cytometry immunophenotyping with pathology/ immunohistochemistry lymph nodes or nodules in diagnosis lymphoproliferative diseases.A retrospective study was carried out 157 biopsy fine-needle aspiration nodes/ nodule specimens taken from 142 patients, 1999 and 2009. The were simultaneously studied fow pathology at Hospital Israelita Albert Einstein. prepared hematoxylin/eosin, Giemsa, monoclonal antibody stained slides for detecting specific...
To discuss the implementation of technical advances in laboratory diagnosis and monitoring paroxysmal nocturnal hemoglobinuria for validation high-sensitivity flow cytometry protocols.A retrospective study based on analysis data from 745 patient samples submitted to and/or hemoglobinuria.Implementation reduced test costs improved resolution clone detection.High-sensitivity allowed more sensitive determination type size, particularly with small clones.Discutir as melhorias técnicas no...