A5029267769- Lymphoma Diagnosis and Treatment
- Acute Myeloid Leukemia Research
- Chronic Lymphocytic Leukemia Research
- Acute Lymphoblastic Leukemia research
- Hematopoietic Stem Cell Transplantation
- Immune Cell Function and Interaction
- Immunodeficiency and Autoimmune Disorders
- Single-cell and spatial transcriptomics
- Chronic Myeloid Leukemia Treatments
- Glioma Diagnosis and Treatment
- Brain Metastases and Treatment
- Viral-associated cancers and disorders
- CNS Lymphoma Diagnosis and Treatment
- Glycosylation and Glycoproteins Research
- Reproductive System and Pregnancy
- Endometriosis Research and Treatment
- T-cell and B-cell Immunology
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Cancer-related cognitive impairment studies
- Traditional and Medicinal Uses of Annonaceae
- Mast cells and histamine
- Eosinophilic Disorders and Syndromes
- Endometrial and Cervical Cancer Treatments
- Hematological disorders and diagnostics
Hospital Israelita Albert Einstein
2014-2024
Instituto de Ensino e Pesquisa Santa Casa
2018
Universidade de São Paulo
2013-2014
Abstract Immunophenotyping by flow cytometry is an integral part of the diagnosis and classification leukemias/lymphomas. The expression ROR1 associated with chronic B lymphocytic leukemia (CLL) well described in literature, both its follow‐up minimal residual disease (MRD) research, however, there are few studies regarding pattern other subtypes mature lymphoid neoplasms. With aim evaluating associating it important markers for differentiation neoplasms (MBLN), 767 samples cases that...
Introduction: Infiltration of the central nervous system by hematologic or lymphoid malignant cells can cause extensive neurological damage, be progressive and fatal. However, usually, cerebrospinal fluid (CSF) has low cellularity rapid cell degeneration, which impair cytometry analysis. Storage transport measures, sample preparation staining protocols interfere with diagnostic accuracy. Objective: To calculate performance flow using a stabilizer for preservation compared to cytomorphology...
Abstract Introduction The workflow in clinical flow cytometry laboratories must constantly be reviewed to develop technical procedures that improve quality and productivity reduce costs. Using the Beckman Coulter dry coating technology, we customized a ten‐color tube with dried antibody reagents, designated Duraclone screening ( DST ), for hematological malignancies. Here, compared applicability, numerical equivalence, cost time required between liquid reagents . Methods contains CD 4 +...
Abstract Background Minimal residual disease (MRD) in chronic lymphocytic leukemia (CLL) has prognostic and predictive significance. One of the approaches to detect MRD by flow cytometry (FC) is use dry antibody reagents such as DuraClone® RE CLB (Beckman Coulter—BC). The aim this study was evaluate performance detecting CLL compared liquid reagents. Methods composed CD81FITC, ROR1PE, CD79bPC5.5, CD19PC7, CD5APC, CD43APCA750, CD20PB, CD45KrO. For reagent assay, we used CD43FITC, CD3ECD,...
Normal B lymphoid maturation occurs in bone marrow (BM) throughout life, but immature B-cell progenitors (BCPs) are more numerous children than adults. To assess the normal values according to age became important as BCPs decreased myelodysplastic syndromes and have been considered an diagnostic prognostic feature these clonal disorders.in a multicenter retrospective study from Brazilian Group of Flow Cytometry we analyzed variation BM technical peculiarities each laboratory. We analysed 45...
Cancer development results from the progressive accumulation of genomic abnormalities that culminate in neoplastic phenotype. Cytogenetic alterations, mutations and rearrangements may be considered as molecular legacy which trace clonal history disease. Concomitant tumors are reported they derive a common or divergent founder clone. B-cell chronic lymphocytic leukemia (B-CLL) plasma cell myeloma (PCM) both mature neoplasms, their concomitancy, albeit rare, is documented. Here, we described...
The Brazilian Group of Flow Cytometry (Grupo Brasileiro de Citometria Fluxo [GBCFLUX]), founded on April 24, 2010, is composed experts in flow cytometry (FC) area who have the common objective contributing to technical and scientific advances clinical research laboratories. Among GBCFLUX working groups, Quality Control (QC) subcommittee responsible for discussing data literature contributes quality assurance pre-analytical, analytical post-analytical process FC. QC subcommittee's actions...
O alcalóide azafluorenona 5,8-dimetóxi-7-hidróxi-1-metil-4-azafluoren-9-ona e o megastigmano (-)-(5R*, 6S*)-megastigman-3-ona-10,7-olidio foram isolados das partes aéreas de Unonopsis lindmanii (Annonaceae), juntamente com os compostos conhecidos (3S*, 5S*, 8R*)-3,5-dihidróxi-megastigma-6,7-dien-9-ona (grasshopper ketone), N-trans-feruloiltiramina, (-)-anonaína, (-)-asimilobina, liriodenina (-)-siringaresinol.Este é primeiro relato da presença megastigmanos em Annonaceae.As estruturas dos...
To demonstrate the advantages of correlating flow cytometry immunophenotyping with pathology/ immunohistochemistry lymph nodes or nodules in diagnosis lymphoproliferative diseases.A retrospective study was carried out 157 biopsy fine-needle aspiration nodes/ nodule specimens taken from 142 patients, 1999 and 2009. The were simultaneously studied fow pathology at Hospital Israelita Albert Einstein. prepared hematoxylin/eosin, Giemsa, monoclonal antibody stained slides for detecting specific...
To discuss the implementation of technical advances in laboratory diagnosis and monitoring paroxysmal nocturnal hemoglobinuria for validation high-sensitivity flow cytometry protocols.A retrospective study based on analysis data from 745 patient samples submitted to and/or hemoglobinuria.Implementation reduced test costs improved resolution clone detection.High-sensitivity allowed more sensitive determination type size, particularly with small clones.Discutir as melhorias técnicas no...
Hospital Israelita Albert Einstein, Sao Paulo, SP, Brazil.Corresponding author: Welbert Oliveira Pereira – Avenida 627/701 − Morumbi Zip code: 05652-900 Brazil Phone: (55 11) 2151-3512 E-mail: welbert.pereira@einstein.brReceived on: Sep 8, 2015 Accepted Nov 4, 2015DOI: 10.1590/S1679-45082016AI3516
Although chronic lymphocytic leukemia is basically a B cell disease, its pathophysiology and evolution are thought to be significantly influenced by T cells, as these probably the most important interaction partner of neoplastic participating in their expansion, differentiation survival. Chronic cells may also drive functional phenotypic changes non-malignant cells. There few data about association between memory prognosis, especially related ZAP-70, common reliable surrogate gold standard...
ABSTRACT Flow cytometry (FC) is an essential tool for diagnosis, prognosis and therapeutic follow-up of several hematologic malignancies. In addition, it performs the quantification lymphocytes subpopulations diagnosis monitoring primary acquired immunodeficiencies through antigenic expressions CD19 CD20 B lymphocytes; CD2, CD3, CD4, CD8 T CD56 CD16 identification natural killer (NK) cells. The technique has revolutionized way that cells are identified, over years this platform progressed...
Objective To validate multilineage score system correlating results of flow cytometry, cytogenetics, cytomorphology and histology from samples patients with suspected myelodysplastic syndrome or cytopenia unknown origin. Methods A retrospective study analyzing laboratory data 49 origin, carried out between May September 2017. The inclusion criteria were availability cytometry results, at least one more method, such as morphology, cytogenetics. Thirty-eight classified [...]
Abstract Introduction Minimal residual disease (MRD) is a cornerstone for stratification of upfront B‐lymphoblastic leukemia (B‐ALL) treatment protocols to decrease relapse risk. Although its detection by flow cytometry (FC) and real‐time quantitative polymerase has clinical usefulness, evidence suggests that methods with increased sensitivity could lead improved outcomes. The aim this study was develop an amplicon‐based assay followed high‐throughput sequencing the immunoglobulin heavy...
Introdução: A linhagem das plaquetas tem sido amplamente investigada na sepse, uma vez que anormalidades no sistema hemostático são eventos comuns nesses pacientes. O objetivo deste estudo foi avaliar a adesão, ativação e agregação plaquetária em pacientes sépticos internados Unidades de Terapia Intensiva durante pandemia COVID-19 por meio da expressão marcadores membrana plaquetária. Método: função 36 início recente 8 controles Junho Setembro 2020 um grande hospital público. Foi obtido...