A5059394086- Immunodeficiency and Autoimmune Disorders
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Systemic Lupus Erythematosus Research
- Eosinophilic Disorders and Syndromes
- Mosquito-borne diseases and control
- Biochemical and Molecular Research
- Science and Education Research
- Prenatal Screening and Diagnostics
- Lipoproteins and Cardiovascular Health
- Acute Myeloid Leukemia Research
- Inflammatory Bowel Disease
- T-cell and Retrovirus Studies
- Blood groups and transfusion
- Venous Thromboembolism Diagnosis and Management
- Vasculitis and related conditions
- Hepatitis B Virus Studies
- Renal Diseases and Glomerulopathies
- Viral Infections and Outbreaks Research
- Vitamin D Research Studies
- Dietary Effects on Health
- Diabetes and associated disorders
- Hepatitis C virus research
- Cancer Genomics and Diagnostics
Hospital Israelita Albert Einstein
2016-2025
Hospital São Paulo
2011-2023
Universidade de São Paulo
2006-2017
Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo
2009-2014
Diabetes mellitus (DM) burden encompasses diabetic kidney disease (DKD), the leading cause of end-stage renal worldwide. Despite compelling evidence indicating that pharmacological intervention curtails DKD progression, search for non-pharmacological strategies can identify novel targets drug development against metabolic diseases. One those emergent comprises modulation intestinal microbiota through fecal transplant from healthy donors. This study sought to investigate benefits (FMT) on...
Abstract We detected Zika virus in breast milk of a woman Brazil infected with the during 36th week pregnancy. Virus was 33 days after onset signs and symptoms 9 delivery. No abnormalities were found fetal assessment or birth infant.
Rivaroxaban is an oral direct factor Xa inhibitor, therapeutically indicated in the treatment of thromboembolic diseases. As other new anticoagulants, routine monitoring rivaroxaban not necessary, but important some clinical circumstances. In our study a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was validated to measure plasmatic concentration. Our used simple sample preparation, protein precipitation, and fast chromatographic run. It developed...
Hepatitis B virus (HBV) is a major cause of chronic liver disease worldwide. Besides genotype, quantitative analysis HBV infection extensively used for monitoring progression and treatment. Affordable viral load desirable in resource-limited settings it has been already shown to be useful developing countries other viruses such as C (HCV) HIV. In this paper, we describe the validation real-time PCR assay DNA quantification with TaqMan chemistry MGB probes. Primers probes were designed using...
O IV Consenso Brasileiro para Pesquisa de Autoanticorpos em Células HEp-2 (FAN) realizado Vitória (ES), no dia 18 setembro 2012, objetivou discutir es- tratégias e recomendações relacionadas ao procedimento técnico, à padronização interpretação dos resultados da pesquisa autoanticorpos células HEp-2. Participaram do evento 23 pesquisadores especialistas Universidades laboratórios brasileiros. Foram abordados diferentes tópicos, discutidos amplamente a fim se estabelecer específicas. integrou...
OBJETIVO:O 3º Consenso Brasileiro para pesquisa de autoanticorpos em Células HEp-2 (FAN) teve como propósito avaliar as dificuldades implantação do 2º ocorrido no ano 2002, discutir estratégias controlar a qualidade ensaio e promover atualização das associações clínicas dos diversos padrões. MÉTODOS:Participaram encontro Goiânia nos dias 13 14 abril 2008 pesquisadores especialistas centros universitários laboratórios clínicos diferentes regiões Brasil, com o aprovar recomendações que visam à...
The objective of this study was to evaluate the presence anti-C1q antibodies Hospital Israelita Albert Einstein Research Institute, São Paulo, Brazil in 67 juvenile systemic lupus erythematosus (JSLE) patients and 26 healthy controls assess association these with disease activity, nephritis, anti-double-stranded (ds)DNA. Anti-C1q were detected by ELISA. A higher frequency observed JSLE compared (20% vs. 0%, P = 0.016). Specificity 100%[95% confidence interval (CI) 86.7-100%] sensitivity...
Our objective was to evaluate the frequency of antinucleosome antibodies (anti-Ncs) in juvenile systemic lupus erythematosus (JSLE) comparing it that observed for anti-DNA and correlate presence these with clinical manifestations disease activity. Anti-Ncs were detected by ELISA 74 patients JSLE 64 normal controls. Clinical records reviewed. Disease activity assessed SLEDAI score. showed sensitivity 52.7% 54% specificity 98.4% 95.3%, respectively. Disagreement between two assays found 25.7%...
OBJETIVO: Avaliar a presença de anticorpos anti-C1q, anticromatina/nucleossomo e anti-DNA duplo filamento (dsDNA) em pacientes com lúpus eritematoso sistêmico juvenil (LESJ) controles. MÉTODOS: Foram analisados 67 LESJ 34 controles saudáveis para anti-dsDNA pelo método ELISA. Os níveis C1q foram avaliados por imunodifusão radial. RESULTADOS: Na época, média idade era similar entre os (14,6 ± 3,86 vs. 13,6 2,93 anos; P = 0,14). observadas frequências mais altas relação aos (20% 0%; 0,0037;...
COVID-19 patients on mechanical ventilation are at risk to develop invasive aspergillosis. To provide additional data regarding this intriguing entity, we conducted a retrospective study describing factors, radiology and prognosis of emerging entity in Brazilian referral centre.This included intubated (≥18 years) with admitted from April 2020 until July 2021 that had bronchoscopy investigate pulmonary co-infections. COVID-19-associated aspergillosis (CAPA) was defined according the European...
OBJETIVO: O Segundo Consenso Brasileiro de Fator Antinuclear (FAN) em Células HEp-2 ratificou os algoritmos decisão para leitura dos padrões do FAN na imunofluorescência indireta vistos primeira edição Brasileiro, adicionando ainda um novo algoritmo relacionado com mistos. MÉTODOS: Tendo vista a habilidade teste detectar autoantígenos nos distintos compartimentos celulares, e não apenas no núcleo, propõe-se novas denominações este exame laboratorial. RESULTADOS E CONCLUSÕES: Como algumas...
Abstract Background The V Brazilian Consensus for determination of autoantibodies against cellular constituents on HEp-2 cells, held in Brasilia (DF, Brazil) August 27, 2016, discussed the harmonization between ANA (BCA) guidelines and International Patterns (ICAP) recommendations ( www.anapatterns.org ). Initial were formulated by group experts with purpose guiding enabling clinical laboratories to adopt provide a common standard national international consensuses. Mainbody Twenty...
Takayasu arteritis (TAK) is a large-vessel granulomatous vasculitis; the inflammatory infiltration in arteries comprises macrophages, multi-nucleated giant cells, CD4+ and CD8+ T γδ natural killer (NK) cells neutrophils. However, it unknown which subtype of macrophages predominates. This study aims to evaluate subpopulations aorta TAK. Immunohistochemistry was performed from TAK patients (n = 22), with atherosclerotic disease 9) heart transplant donors 8) using markers CD68, CD86, CD206,...
Objective To test and validate a multiplex real-time polymerase chain reaction method for bloodstream infections, as well to compare the results with conventional blood culture.Methods A total of 114 consecutive patients clinical evidence sepsis were submitted culture LightCycler™ SeptiFast tests.Results More positive specimens (23; 20.2%) detected using than (17; 14.9%), an agreement 86.8%. Discordant seen in four only culture, ten one different pathogens found by each test. Infections...
To evaluate the frequencies of iNKT cells and their subsets in patients with deep endometriosis.A case-control study was conducted between 2013 2015, 73 distributed into two groups: 47 women a histological diagnosis endometriosis 26 controls. Peripheral blood, lesions, healthy peritoneal samples were collected on day surgery to determine subtypes via flow cytometry analysis.The authors observed lower number (p = 0.01) Double-Negative (DN) 0.02) blood than control group. The DN IL-17+...