A5023777673- Synthesis of Organic Compounds
- Natural Compound Pharmacology Studies
- Fatty Acid Research and Health
- Venous Thromboembolism Diagnosis and Management
- Lipoproteins and Cardiovascular Health
- Biological Activity of Diterpenoids and Biflavonoids
- Cancer, Lipids, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Endometriosis Research and Treatment
- Antioxidant Activity and Oxidative Stress
- Lipid metabolism and biosynthesis
- Estrogen and related hormone effects
- Atrial Fibrillation Management and Outcomes
- Metabolomics and Mass Spectrometry Studies
- Blood properties and coagulation
- HIV/AIDS drug development and treatment
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Phytochemistry Medicinal Plant Applications
- biodegradable polymer synthesis and properties
- Reproductive System and Pregnancy
- Endometrial and Cervical Cancer Treatments
- Cardiac electrophysiology and arrhythmias
- Crystallization and Solubility Studies
- Bioactive Compounds and Antitumor Agents
- Diabetes, Cardiovascular Risks, and Lipoproteins
Hospital Israelita Albert Einstein
2015-2021
Universidade de São Paulo
2011-2019
Universidade Federal de Alfenas
2007-2012
We investigated whether palmitoleic acid, a fatty acid that enhances whole body glucose disposal and suppresses hepatic steatosis, modulates triacylglycerol (TAG) metabolism in adipocytes. For this, both differentiated 3T3-L1 cells treated with either (16:1n7, 200 μM) or palmitic (16:0, for 24 h primary adipocytes from wild-type PPARα-deficient mice 16:1n7 (300 mg·kg(-1)·day(-1)) oleic (18:1n9, 300 by gavage 10 days were evaluated lipolysis, TAG, glycerol 3-phosphate synthesis gene protein...
Docosahexaenoic acid (DHA) is an n-3 polyunsaturated fatty that highly enriched in the brain, and oxidation products of DHA are present or increased during neurodegenerative disease progression. The characterization critical to understanding roles these play development such diseases. In this study, we developed a sensitive specific analytical tool for detection quantification twelve major hydroperoxide (HpDoHE) hydroxide (HDoHE) isomers (isomers at positions 4, 5, 7, 8, 10, 11, 13, 14, 16,...
Rivaroxaban is an oral direct factor Xa inhibitor, therapeutically indicated in the treatment of thromboembolic diseases. As other new anticoagulants, routine monitoring rivaroxaban not necessary, but important some clinical circumstances. In our study a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was validated to measure plasmatic concentration. Our used simple sample preparation, protein precipitation, and fast chromatographic run. It developed...
Endometriosis is an estrogen-dependent disease affecting up to 10% of all premenopausal women. There evidence that different endometriosis sites show distinct local estrogen concentration, which, in turn, might be due a unique metabolism. We aimed investigate whether there was site-specific regulation selected enzymes responsible for the oxidative metabolism estrogens biopsy samples and endometrial endometriotic stromal cells. Cytochrome P450 (CYP) 1A1 CYP1B1 mRNA protein expressions...
Abstract This article reports the structural elucidation by IR, UV and MS spectroscopic data along with 1 H 13 C NMR chemical shift assignments of two benzophenones isolated from fruit pericarp Garcinia brasiliensis Mart. (Clusiaceae): garciniaphenone, (1 R ,5 S ,7 )‐3‐benzoyl‐4‐hydroxy‐6,6‐dimethyl‐5,7‐di(3‐methyl‐2‐butenyl)bicyclo[3.3.1]non‐3‐ene‐2,9‐dione, a novel triprenylated benzophenone; 7‐ epi ‐clusianone, tetraprenylated benzophenone that has already been extracted another species...
The keto-enol tautomerism and stereochemistry study of a HIV-inhibitory natural benzophenone, (1R,5R,7R,8S)-(+)-3-(10-(3,4-dihydroxyphenyl)-10-hydroxymethylene)-8-methyl -1,5,7-tris(3-methyl-2-butenyl)-8-(4-methyl-3-pentenyl)-bicyclo[3.3.1]nonane-2,4,9-trione (a), isolated from Garcinia brasiliensis seeds is presented. crystal structure which also know as guttiferona A, was determined by X-ray diffraction its intra inter-molecular geometries discussed compared with two analogue...
This paper describes the investigation of cytotoxic and antioxidant activities in vivo mutagenic/antimutagenic potential different concentrations hexane extract (EHP) isolated molecule 7-epi-clusianone (MI) Rheedia brasiliensis. The vitro activity MI was investigated by monitoring reduction radical scavenging metal chelating DPPH (1,1-diphenyl-2-picryl-hydrazil). Cytotoxic assessed measuring mortality brine shrimp presence absence compounds. mutagenic, antimutagenic effects these compounds...
Despite defenses by polymorphonuclear neutrophils in the host against invading agents, overproduction of oxidant species phagocytes can lead to damage surrounding tissues. Several benzophenones have been shown possess anti-inflammatory properties. The effect natural benzophenone 7-epiclusianone isolated from leaves Garcinia brasiliensis was investigated using vitro antioxidant and ex vivo assays, focusing on neutrophil respiratory burst biochemical pathways involved. bioactive extract,...
For cardiovascular disease prevention, statins alone or combined with ezetimibe have been recommended to achieve low-density lipoprotein cholesterol targets, but their effects on other lipids are less reported. This study was designed examine lipid changes in subjects ST-segment elevation myocardial infarction (STEMI) after two highly effective lipid-lowering therapies. Twenty patients STEMI were randomized be treated rosuvastatin 20 mg QD simvastatin 40 10 for 30 days. Fasting blood samples...
BIOLOGICAL IMPLICATIONS OF DOCOSAHEXAENOIC ACID IN NEURODEGENERATIVE DISEASES.Docosahexaenoic acid (C22:6, n-3, DHA) is a polyunsaturated fatty (PUFA) present in large concentrations the brain and, due to presence of six double bonds its structure, highly susceptible oxidation by enzymes and reactive oxygen/nitrogen species.The peroxidation PUFAs has been implicated an increasing number human disorders, including neurodegenerative diseases.Hence, better understanding metabolism pathways DHA...
The aim of the study was to evaluate diagnostic accuracy thromboelastometry for assessing rivaroxaban concentrations. compared with high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS) method, which is gold standard drug plasma monitoring (the reference standard). Forty-six clinically stable patients were treated 10, 15, or 20 mg once daily (OD group) 15 twice a day (BID (no particular indication treatment). Patient samples collected 2 h after use medication (peak)...
Rivaroxaban is a direct oral anticoagulant designed to dispense with the necessity of laboratory monitoring. However, monitoring rivaroxaban levels necessary in certain clinical conditions, especially critical care setting.This diagnostic accuracy study evaluating sensitivity and specificity prothrombin time (PT), activated partial thromboplastin (aPTT), Dilute Russell viper venom (dRVVT), evaluate hemorrhagic risk patients taking rivaroxaban. The used convenience sample 40 clinically stable...
Abstract Introduction For cardiovascular disease prevention, statins alone or combined with ezetimibe have been recommended to achieve low density lipoprotein - cholesterol targets, but their effects on other lipids are less reported. Objective To examine lipid changes in subjects ST-segment elevation myocardial infarction (STEMI) after two highly effective lipid-lowering therapies. Methods Twenty patients STEMI were randomized be treated rosuvastatin 20 mg qd simvastatin 40 10 during 30...