Dipeptidyl peptidase IV (DPP-IV), a new target for the treatment of type 2 diabetes mellitus, degrades incretins such as glucagon-like peptide 1 (GLP-1) and glucose-dependent insulinotropic polypeptide. DPP-IV inhibitors shorten inactivation GLP-1, permitting incretin to stimulate insulin release, thereby combating hyperglycemia. In our ongoing search from medicinal plants foods, three flavonol glycosides (1⁻3) were isolated seeds Lens culinaris Medikus (Fabaceae) tested their...

Chrysanthemum morifolium Ramat is a perennial flowering plant widely cultivated for use in tea infusion and as popular beverage. To identify evaluate the made with γ-irradiated mutant chrysanthemum cultivar dark purple petals (cv. ARTI-Dark Chocolate), its phytochemical composition antioxidant activity were tested compared those of commercially available yellow Gamguk) by HPLC-DAD-ESIMS, well DPPH ABTS radical scavenging assays. The contained anthocyanins linarin, which not detected content...

Coreopsis is a flowering plant belonging to the Asteraceae family. It an ornamental native Americas, Asia and Oceania its flower used as raw material for tea food manufacture in China. In this study, new cultivars of C. rosea ("golden ring") were developed via radiation-induced mutation original cultivar, "pumpkin pie". The chemical composition antioxidant activities flowers three different evaluated: "golden ring", pie" "snow chrysanthemum" (coreopsis tea; tinctoria). volatile compounds...

The flowers of Pueraria lobate (Puerariae Flos) have served as a traditional Chinese medicinal and food herbage plant for many years. Tectoridin is one the most active metabolites extracted from has variety beneficial activities, including antioxidative, hypoglycemic, anti-inflammatory activities. Nevertheless, functions potential mechanisms underlying tectoridin in cerebral ischemia/reperfusion injury not been well interpreted; thus, network analysis strategy was performed to systematically...

Objectives: Ischemic stroke is a severe neurological disorder with high morbidity, mortality, and disability rates, posing substantial burden on patients, families, healthcare systems. Soy isoflavone (SI), naturally occurring phytoestrogen, has demonstrated promising neuroprotective effects. This study aimed to evaluate the anti-stroke efficacy of SI elucidate its underlying mechanisms through integrated phytochemical profiling, network pharmacology, both in vitro vivo experimental...

The aim of this study was to investigate the protective ability blackberry extract (BE) against oxidative stress in carbon tetrachloride (CCl4)-treated rats. results showed that treatment with BE attenuated lipid peroxidation increased by CCl4 and also markedly recovered activity antioxidant enzymes, such as superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), reductase (GR), were decreased CCl4. elevated protein expression levels NF-E2-related factor-2 (Nrf2), CuZnSOD,...

Mangostenone F (MF) is a natural xanthone isolated from Garcinia mangostana. However, little known about the biological activities of MF. This study was designed to investigate anti-inflammatory effect and underlying molecular mechanisms MF in lipopolysaccharide (LPS)-stimulated RAW264.7 macrophages. dose-dependently inhibited production NO, iNOS, pro-inflammatory cytokines (TNF-α, IL-6, IL-1β) LPS-stimulated Moreover, decreased NF-κB luciferase activity DNA binding capacity Furthermore,...

Isoegomaketone (IK) was isolated from Perilla frutescens, which has been widely used as a food in Asian cuisine, and evaluated for its biological activity. We have already confirmed that IK induced the HO-1 expression via Nrf2 activation RAW264.7 cells. In this study, we investigated effect of on mechanism expression. upregulated mRNA protein dose dependent manner. The level peaked at 4 h after 15 μM treatment. To investigate mechanisms modulation by IK, pharmacological inhibitors kinase C...

The flowers of chrysanthemum species are used as a herbal tea and in traditional medicine. In addition, members the genus have been selected to develop horticultural cultivars diverse floral colors capitulum forms. this research, we investigated phytochemical composition eight gamma-irradiation mutant Chrysanthemum morifolium their original cultivars. were generated by treatment with various doses 60Co gamma irradiation stem cuttings three commercial follows: 'ARTI-Dark Chocolate' (50Gy),...

Hibiscus species are rich in phenolic compounds and have been traditionally used for improving human health through their bioactive activities. The present study investigated the of leaf extracts from 18 different H. acetosella accessions evaluated biofunctional properties, focusing on antioxidant antibacterial activity. most abundant compound was caffeic acid, with levels ranging 14.95 to 42.93 mg/100 g. activity measured by ABTS assay allowed be classified into two groups: a high group red...

Twenty-one angular dihydropyranocoumarins and a linear furanocoumarin, including four previously undescribed compounds (1-4), were isolated from the flowers of Peucedanum japonicum (Umbelliferae). The structures 1-4, along with their absolute stereochemistry, determined to be (3'S,4'S)-3'-O-propanoyl-4'-O-(3‴-methyl-2‴-butenoyl)khellactone (1), (3'S,4'S)-3'-O-propanoyl-4'-O-(2‴-methyl-2‴Z-butenoyl)khellactone (2), (3'S,4'S)-3'-O-propanoyl-4'-O-(2‴-methylbutanoyl)khellactone (3),...

In this study, we investigated the anti-inflammatory effect of rosmarinic acid methyl ester (RAME) isolated from a mutant cultivar Perilla frutescens (L.) Britton. We found that RAME inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production, with an IC50 14.25 µM, in RAW 264.7 cells. inhibited LPS-induced expression pro-inflammatory cytokines including interleukin (IL)-1β, IL-6, IL-10, monocyte chemoattractant protein-1, interferon-β, and inducible synthase (iNOS). Moreover,...

Jaihyunk Ryu, Soon-Jae Kwon, Yeong Deuk Jo, Chang Hyun Jin, Bo Mi Nam, Seung Young Lee, Sang Wook Jeong, Bin Im, Cheol Oh, Lan Cho, Bo-Keun Ha, and Si-Yong Kang. Plant Breeding Biotechnology 2016;4:242-51. https://doi.org/10.9787/PBB.2016.4.2.242

A new polyacetylene glycoside, (5R)-6E-tetradecene-8,10,12-triyne-1-ol-5-O-β-glucoside (1), was isolated from the flower of Coreopsis lanceolata (Compositae), together with two known compounds, bidenoside C (10) and (3S,4S)-5E-trideca-1,5-dien-7,9,11-triyne-3,4-diol-4-O-β-glucopyranoside (11), which were found in species for first time. The other lanceoletin (2), 3,2′-dihydroxy-4-3′-dimethoxychalcone-4′-glucoside (3), 4-methoxylanceoletin (4), lanceolin (5), leptosidin (6),...

Isoegomaketone (IK) is an essential oil component of Perilla frutescens (L.) Britt., and there have been no studies investigating its biological activities. We found that IK inhibits lipopolysaccharide (LPS)-induced nitric oxide (NO) production in RAW 264.7 macrophages, moreover, when was injected into animals prior to LPS administration, NO serum levels decreased a dose-dependent manner. These results indicate possesses anti-inflammatory activity both vitro vivo. suppressed the...

Isoegomaketone (IK) is an essential oil component of Perilla frutescens (L.), but the mechanism by which IK induces apoptosis has never been studied. The purpose this study was to elucidate IK-induced apoptotic pathway in DLD1 human colon cancer cells. We observed that treatment over 24 h significantly inhibited cell viability a dose-dependent manner. also found triggered cleavage PARP. Moreover, resulted caspase-8, -9, and -3 dose- time-dependent Bid translocation Bax, release cytochrome c...

Dendrobii Herba is an herbal medicine that uses the stems of Dendrobium species (Orchidacea). It has been traditionally used to treat fever, hydrodipsomania, stomach disorders, and amyotrophia. In our previous study, a bibenzyl compound, moscatilin, which isolated from Herba, showed potent cytotoxicity against FaDu human pharyngeal squamous carcinoma cell line. Prompted by this finding, we performed additional studies in cells investigate mechanism action. Moscatilin induced death using 5 μM...

The flowers of Chrysanthemum morifolium Ramat. have been used as an herbal tea and in traditional medicine, the plant has developed to produce horticultural cultivars various colors shapes. In this study, a new chrysanthemum cultivar with dark purple petals ( C. cv. ARTI-Dark Chocolate; ADC) was by radiation-induced mutation breeding its original striped white Noble Wine, NW). phenolic profile antioxidant property ADC were investigated compared NW commercially available medicinal herb,...

The goal of this study was to identify phytochemicals with inhibitory activity against tyrosinase. Nine compounds 1-9 were isolated from the tubers Apios americana. This is first report aromadendrin 5-methyl ether (1) being species. Among them, 2 and 8 showed toward Based on a Dixon plot, potential Ki values competitive inhibitors calculated as 10.3 ± 0.8 µM 44.2 1.7 µM, respectively. An IC50 value 13.2 1.0 for slow-binding inhibitor after preincubation Additionally, predicted binding sites...

Human leucocyte antigen (HLA)-G is an important molecule for maintaining immunotolerant foetal-maternal relationship. A 14-bp insertion (+14-bp)/deletion (-14-bp) polymorphism in exon 8 of the 3' untranslated region HLA-G gene has been proposed to be associated with mRNA stability and expression HLA-G. This might play immunomodulatory role human pregnancy. In this study, a total 526 Chinese women were genotyped +14-bp/-14-bp polymorphism, including 137 who had recurrent miscarriages (three...

The leaves of Perilla frutescens var. crispa (Lamiaceae)—known as ‘Jureum-soyeop’ or ‘Cha-jo-ki’ in Korean, ‘ZI SU YE’ Chinese, and ‘Shiso’ Japan—has been used a medicinal herb. Recent gamma irradiated mutation breeding on P. our research group resulted the development new perilla cultivar, (cv. Antisperill; PFCA), which has higher content isoegomaketone. PFCA were extracted by supercritical carbon dioxide (SC-CO2) extraction, phytochemical investigation this extract led to isolation...