Despite the growing understanding of mechanisms carcinogenesis, cancers central nervous system are usually associated with unfavorable prognosis. The use an appropriate molecular marker may improve treatment outcome by allowing early diagnosis and susceptibility monitoring. Since methylation tumor-derived DNA can be detected in serum cancer patients, this makes methylation-based biomarkers one most promising diagnostic strategies. In study, profiles MGMT, RASSF1A, p15INK4B, p14ARF genes were...

Lichens are a source of secondary metabolites which possess important biological activities, including antioxidant, antibacterial, anti-inflammatory, and cytotoxic effects. The anticancer activity lichens was shown in many types tumors, colorectal cancers (CRC). Several studies revealed that the application lichen extracts diminished proliferation CRC cells induced apoptosis. Colon carcinogenesis is associated with aberrations Wnt signaling. Elevated transcriptional β-catenin induces cell...

Lichens are a source of secondary metabolites with significant pharmacological potential. Data regarding their possible application in glioblastoma (GBM) treatment are, however, scarce. The study aimed at analyzing the mechanism action six lichen metabolites: atranorin, caperatic acid, physodic squamatic salazinic and lecanoric acid using two- three-dimensional GBM cell line models. parallel artificial membrane permeation assay was used to predict blood-brain barrier penetration ability...

Photoaging is a process related to an increased level of reactive oxygen species (ROS). Polyphenols can scavenge free radicals in the body, which delay skin aging. Therefore, our work aimed prepare biologically active extract from dry fruits Vaccinium myrtillus or corymbosum and use it for preparation hydrogels topical application. eight different extracts (using V. extraction mixtures: methanol, methanol–water 1:1, water, acetone–water 1:1) were prepared their phytochemical (total...

Activation of the Wnt pathway contributes to development head and neck squamous cell carcinomas (HNSCC) its inhibition has recently emerged as a promising therapeutic strategy. Here, we aimed at identifying suitable molecular targets for down-regulation canonical signaling in HNSCC cells. Candidate target genes (PORCN, WNT3A, FZD2, FZD5, LRP5, DVL1, CIP2A, SET, KDM1A, KDM4C, KDM6A, CBP, CARM1, KMT2A, TCF7, LEF1, PYGO1, XIAP) were silenced using siRNA selected subsequently blocked small...

Abstract Background Glioblastoma (GBM) is the deadliest and most common primary brain tumor in adults. The invasiveness proliferation of GBM cells can be decreased through inhibition Wnt/β-catenin pathway. In this regard, celecoxib a promising agent, but other COXIBs 2,5-dimethylcelecoxib (2,5-DMC) await elucidation. Thus, aim study was to analyze impact celecoxib, 2,5-DMC, etori-, rofe-, valdecoxib on cell viability activity addition, combination compounds with temozolomide (TMZ) also...

The Wnt/β-catenin, EGFR, and PI3K pathways frequently undergo upregulation in head neck squamous carcinoma (HNSCC) cells. Moreover, the Wnt/β-catenin pathway together with Hedgehog (Hh) signaling regulate activity of cancer stem cells (CSCs). aim this study was to investigate effects combinatorial use Hh inhibitors on viability, cell cycle progression, apoptosis induction, migration, expression CSC markers tongue (CAL 27) hypopharynx (FaDu) Co-inhibition Wnt EGFR or additionally tested. were...

Background. Cancer cells are dependent on aerobic glycolysis for energy production and increased glutamine consumption. HIF-1α c-MYC transcription factors regulate the expression of glycolytic glutaminolytic genes. Their activity may be repressed by SIRT6. Head neck carcinomas show frequent activation function SIRT6 down-regulation, which contributes to a strong dependence glucose availability.Objectives. The aim this study was compare influence inhibitors (KG-548 10058-F4, respectively)...

Novel therapeutics are required to improve treatment outcomes in head and neck squamous cell carcinoma (HNSCC) patients. Histone lysine demethylases (KDM) have emerged recently as new potential drug targets for HNSCC therapy. They might also potentiate the action of inhibitors EGFR PI3K signaling pathways. This study aimed at evaluating anti-cancer effects KDM4 (ML324) KDM6 (GSK-J4) their combinations with (erlotinib) (HS-173) cells. The effect on viability CAL27 FaDu cells was evaluated...

Glioblastoma (GBM) is an extremely aggressive brain tumor awaiting novel, efficient, and minimally toxic treatment. Curcuminoids (CCM), polyphenols from Curcuma longa, sodium butyrate (NaBu), a histone deacetylase inhibitor naturally occurring in the human body, await elucidation as potential anti-GBM agents. Thus, aim of this study was to analyze CCM NaBu both separately combination treatment using three GBM cell lines. MTT used for cytotoxicity evaluation, index calculated synergism...

PARP inhibitors are used to treat cancers with a deficient homologous recombination (HR) DNA repair pathway. Interestingly, recent studies revealed that HR could be pharmacologically impaired by the inhibition of histone lysine demethylases (KDM). Thus, we investigated whether KDM sensitize head and neck cancer cells, which usually proficient, or cisplatin. Therefore, explored effects double combinations KDM4–6 (ML324, CPI-455, GSK-J4, JIB-04) olaparib cisplatin, their triple both drugs, on...

Epigenetic changes play an important role in the pathogenesis of gliomas and have potential to become clinically useful biomarkers. The aim this study was evaluation profile promoter methylation 13 genes selected based on their anticipated diagnostic and/or prognostic value. Methylation-specific PCR (MSP) used assess status MGMT, ERCC1, hMLH1, ATM, CDKN2B (p15INK4B), p14ARF, CDKN2A (p16INK4A), RASSF1A, RUNX3, GATA6, NDRG2, PTEN, RARβ a subset 95 different grades. Additionally, MGMT NDRG2...

Background: Increasing evidence suggests that combinations of phytochemicals are more efficient than single components in the modulation signaling pathways involved cancer development. In this study, impact phenethyl isothiocyanate (PEITC), indole-3-carbinol (I3C), xanthohumol, (X), and resveratrol (RES) their on activation expression Nrf2 NF-κB human hepatocytes HCC cells were evaluated. Methods: THLE-2 HepG2 exposed to for 24 h. The NF-κB, target genes, effect survival assessed. tumor...

Pancreatic cancer is a disease in which deregulation of signaling pathways plays key role, thus searching for their novel modulators promising therapeutic strategy. Hence, this study, the effect phytochemical combinations on canonical and non-canonical activation Nrf2 its interaction with NF-κB pathway was evaluated extensively proliferating pancreatic cell line, PSN-1, comparison to non-cancerous MS1 cells. The NF-κB, expression target genes, survival were assessed PSN-1 tumor burden mice...

Excessive glucose metabolism and disruptions in Wnt signaling are important molecular changes present oral cancer cells. The aim of this study was to evaluate the effects combinatorial use glycolysis inhibitors on viability, cytotoxicity, apoptosis induction, cell cycle distribution glycolytic activity tongue carcinoma CAL 27, SCC-25 BICR 22 lines were used. Cells treated with (2-deoxyglucose lonidamine) (PRI-724 IWP-O1). compounds viability cytotoxicity evaluated MTS CellTox Green tests,...

Glioblastoma is the most common and deadliest brain cancer. The aim of this study was to analyze impact resveratrol its five analogs: 3,4,4′-trimethoxy, 3,4,2′-trimethoxy, 3,4,2′,4′-tetramethoxy, 3,4,2′,6′-tetramethoxy 3,4,2′,4′,6′-pentamethoxy-trans-stilbenes (MS) on human glioblastoma T98G cells. Parallel Artificial Membrane Permeation Assay (PAMPA) used for prediction blood-brain barrier penetration ability tested stilbenes (PAMPA-BBB). MTT test applied cytotoxicity compounds, whereas...

Nrf2 and NF-κB play a key role in inflammation-driven cancers. Conjugation of anti-inflammatory drugs with oleanolic acid oxime (OAO) may enhance their therapeutic potential as result downregulation these pathways. Novel OAO derivatives conjugated indomethacin (IND) were synthesized, effect on the activation expression HepG2 hepatoma cells THLE-2 immortalized normal hepatocytes was evaluated relation to cell cycle arrest apoptosis. Treatment OAO–IND conjugates reduced active forms cells,...

This study aimed to synthesize new thioderivative chalcones and analyze their impact on the NF-κB, STAT3, EGFR Nrf2 signaling pathways in colorectal cancer cells. Among studied compounds, derivatives 4 5 decreased activation of NF-κB expression target gene COX-2. In case we observed inhibition this pathway after influencing derivative 4. Increased was demonstrated for 7 DLD-1 HCT116 The results indicated that chalcone derivatives, especially compounds 4, 5, and-to some degree-7, possess...

Combining NSAIDs with conventional therapeutics was recently explored as a new strategy in cancer therapy. Our earlier studies showed that novel oleanolic acid oximes (OAO) conjugated aspirin or indomethacin may enhance their anti-cancer potential through modulation of the Nrf2 and NF-κB signaling pathways. This study focused on synthesis biological evaluation four diclofenac (DCL)–OAO derivative conjugates context these pathways’ modification hepatic cells survival. Treatment 4d,...

The study aimed to evaluate the possible modulation of Nrf2, NF-ĸB and STAT3 signaling pathways in colorectal cancer (CRC) cells line DLD-1 HCT116 by secondary metabolites lichens. An attempt was made indicate most promising targets these pathways. Attention also paid effects compounds tested on CRC using anakoinosis-that is, simultaneous analysis several natural activity selected transcriptional factors related were analyzed Western blot RT-PCR assays. highest against shown physodic...