A5081361317- Alzheimer's disease research and treatments
- Neuroinflammation and Neurodegeneration Mechanisms
- Microplastics and Plastic Pollution
- Extracellular vesicles in disease
- Dementia and Cognitive Impairment Research
- Cholinesterase and Neurodegenerative Diseases
- Recycling and Waste Management Techniques
- Amyotrophic Lateral Sclerosis Research
- Nanoparticles: synthesis and applications
- Parkinson's Disease Mechanisms and Treatments
- Neurological diseases and metabolism
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Lysosomal Storage Disorders Research
- S100 Proteins and Annexins
- Adenosine and Purinergic Signaling
- Genetic Neurodegenerative Diseases
- Functional Brain Connectivity Studies
- Neurogenetic and Muscular Disorders Research
- Amyloidosis: Diagnosis, Treatment, Outcomes
Centro San Giovanni di Dio Fatebenefratelli
2021-2024
University of Brescia
2021-2022
High repeat expansion (HRE) alleles in
Alzheimer’s disease (AD), frontotemporal dementia (FTD), and with Lewy bodies (DLB) are the three major neurodegenerative dementias. In this study, we provide evidence that an alteration in extracellular vesicles (EVs) release is common across most dementias, AD, DLB, FTD. Specifically, analyzed plasma EVs groups of patients affected by FTD, found a significant reduction concentration larger size all patient groups. We then investigated whether loss neurotrophic factors also pathogenic...
Beta-site APP cleaving enzyme 1 (BACE1) is the rate-limiting in amyloid-β (Aβ) plaques formation. BACE1 activity increased brains of patients with Alzheimer's disease (AD) and mild cognitive impairment (MCI) plasma levels appears to reflect those brains.In this work, we investigated role serum as biomarker for AD, estimating diagnostic accuracy assay assessing correlation Aβ1 - 40, 42, Aβ40/42 ratio serum, known biomarkers brain amyloidosis.Serum were assessed 31 28 MCI, diagnosed AD at...
Alzheimer’s disease (AD), dementia with Lewy bodies (DLB) and frontotemporal (FTD) represent the three major neurodegenerative dementias characterized by abnormal brain protein accumulation. In this study, we investigated extracellular vesicles (EVs) neurotrophic factors in cerebrospinal fluid (CSF) of 120 subjects: 36 AD, 30 DLB, 34 FTD 20 controls. Specifically, CSF EVs were analyzed Nanoparticle Tracking Analysis measured ELISA. We found higher EV concentration lower size AD DLB groups...
Alzheimer's disease (AD) and Frontotemporal lobar degeneration (FTLD) represent the most common forms of neurodegenerative dementias with a highly phenotypic variability. Herein, we investigated role genetic variants related to immune system inflammation as modulators in AD dementias. In patients sporadic AD/FTLD (n = 300) GRN/C9orf72 mutation carriers 80), performed targeted sequencing 50 genes belonging inflammation, selected based on their high expression brain regions low tolerance...
True-to-life nanoplastics as a closer model for environmental pollution.
Cutting-edge research suggests endosomal/immune dysregulation in GRN/C9orf72-associated frontotemporal lobar degeneration (FTLD). In this retrospective study, we investigated plasma small extracellular vesicles (sEVs) and complement proteins 172 subjects (40 Sporadic FTLD, 40 Intermediate/Pathological C9orf72 expansion carriers, 49 Heterozygous/Homozygous GRN mutation 43 controls). Plasma sEVs (concentration, size) were analyzed by nanoparticle tracking analysis; C1q, C4, C3 quantified...
Alzheimer’s disease (AD) is a pathology characterized by the accumulation in brain of intracellular and extracellular amyloid-β (Aβ) aggregates, especially Aβ1–40 Aβ1–42 peptides. It known that N-terminally truncated or modified Aβ forms also exist AD brains cerebrospinal fluid (CSF), they play key role pathogenesis disease. Herein, we developed an antibody-free method based on Solid-Phase Extraction Electrospray Ionization Liquid Chromatography Mass Spectrometry for identification...
The accumulation of protein aggregates defines distinct, yet overlapping pathologies such as Alzheimer's disease (AD), dementia with Lewy bodies (DLB), and frontotemporal (FTD). In this study, we investigated ATG5, UBQLN2, ULK1, LC3 concentrations in 66 brain specimens 120 plasma samples from AD, DLB, FTD, control subjects (CTRL). Protein concentration was measured ELISA kits temporal, frontal, occipital cortex 32 10 14 CTRL, 30 CTRL. We found alterations levels patients; ATG5 UBQLN2 were...
Alzheimer's disease (AD) and frontotemporal dementia (FTD) are the two major neurodegenerative diseases causing dementia. Due to similar clinical phenotypes, differential diagnosis is challenging without specific biomarkers. Beta-site Amyloid Precursor Protein cleaving enzyme 1 (BACE1) a β-secretase pivotal in AD pathogenesis. In mild cognitive impairment subjects, BACE1 activity increased brain/cerebrospinal fluid, plasma levels appear reflect those brain. this study, we aim evaluate serum...
Dysfunctions in the endo-lysosomal system have been hypothesized to underlie neurodegeneration major neurocognitive disorders due Alzheimer's disease (AD), Frontotemporal Lobar Degeneration (FTLD), and Lewy body (DLB). The aim of this study is investigate whether these diseases share genetic variability pathway. In AD, DLB, FTLD patients controls (948 subjects), we performed a targeted sequencing top 50 genes belonging Genetic analyses revealed (i) four previously reported disease-associated...
Emerging data suggest the roles of endo-lysosomal dysfunctions in frontotemporal lobar degeneration (FTLD) and other dementias. Cathepsin D is one major lysosomal proteases, mediating degradation unfolded protein aggregates. In this retrospective study, we investigated cathepsin levels human plasma small extracellular vesicles (sEVs) 161 subjects (40 sporadic FTLD, 33 intermediate/pathological C9orf72 expansion carriers, 45 heterozygous/homozygous GRN mutation 43 controls). was quantified by...
Given the complexity of separating nanoplastics from environmental samples, studies have usually been conducted using synthetic polystyrene nanobeads. By mechanical fragmentation in cryogenic conditions daily-life plastic items, we produced “true-to-life” (T2LNPs), that promises to give a true insight into interaction with biological systems. T2LNPs fully characterized by Fourier transform Infrared spectroscopy and Atomic Force Microscopy. They result populations spheroidal nanoparticles...
Genetic frontotemporal lobar degeneration (FTLD) is characterized by heterogeneous phenotypic expression, with a disease onset highly variable even in patients carrying the same mutation. Herein we investigated if variants lysosomal genes modulate age of both FTLD due to GRN null mutations and C9orf72 expansion. In total 127 subjects (n = 74 n 53 expansion carriers), performed targeted sequencing top 98 belonging pathway, selected based on their high expression multiple brain regions. We...
Given the complexity of separating nanoplastics from environmental samples, studies have usually been conducted using synthetic polystyrene nanobeads. By mechanical fragmentation in cryogenic conditions daily-life plastic items, we produced “true-to-life” (T2LNPs), that promises to give a true insight into interaction with biological systems. T2LNPs fully characterized by Fourier transform Infrared spectroscopy and Atomic Force Microscopy. They result populations spheroidal nanoparticles...