A5050212861- Neonatal Respiratory Health Research
- Pediatric health and respiratory diseases
- Chronic Obstructive Pulmonary Disease (COPD) Research
- IL-33, ST2, and ILC Pathways
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Asthma and respiratory diseases
- COVID-19 Clinical Research Studies
- Medical Imaging and Pathology Studies
- SARS-CoV-2 and COVID-19 Research
- Cancer Immunotherapy and Biomarkers
- Psoriasis: Treatment and Pathogenesis
- Congenital Diaphragmatic Hernia Studies
- Tracheal and airway disorders
- Neuroscience of respiration and sleep
- Cancer Cells and Metastasis
- Helicobacter pylori-related gastroenterology studies
- Immune cells in cancer
- Epigenetics and DNA Methylation
- Pleural and Pulmonary Diseases
- Cystic Fibrosis Research Advances
- Long-Term Effects of COVID-19
- Immune Response and Inflammation
- Venomous Animal Envenomation and Studies
- Single-cell and spatial transcriptomics
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
Saarland University
2017-2025
Helmholtz Institute for Pharmaceutical Research Saarland
2022-2024
Helmholtz Centre for Infection Research
2023-2024
Universitätsklinikum des Saarlandes
2019
Max Rubner Institut
2016
COVID-19 comprises several severity stages ranging from oligosymptomatic disease to multi-organ failure and fatal outcomes. The mechanisms why is a mild in some patients progresses severe often with respiratory are not known. Biomarkers that predict the course of urgently needed. aim this study was evaluate large spectrum established laboratory measurements.Patients prospective PULMPOHOM CORSAAR studies were recruited comprised 35 COVID-19, 23 conventional pneumonia, 28 control undergoing...
Eggerthella and Slackia spp. are gut associated bacteria that have been suggested to play roles in host lipid xenobiotic metabolism. A quantitative PCR method for the selective enumeration of belonging either genus or was developed order establish numbers these occurring human feces. The primers amplification genera were tested first conventional test their specificity. Representative species Slackia, as well closely related Coriobacteriia, included investigation. selected shown be capable...
Chronic obstructive pulmonary disease (COPD) is associated with neutrophilic lung inflammation and CD8 T cell exhaustion an important risk factor for the development of non-small cancer (NSCLC). The clinical response to programmed death-1 (PD-1) blockade in NSCLC patients variable likely affected by a coexisting COPD. pro-inflammatory cytokine interleukin-17C (IL-17C) promotes present human tumors. Here, we used Kras-driven model examine function IL-17C inflammation-promoted tumor growth....
Abstract Chronic lung diseases such as chronic obstructive pulmonary disease and cystic fibrosis are incurable. Epithelial senescence, a state of dysfunctional cell cycle arrest, contributes to the progression diseases. Therefore, epithelial cells valuable target for therapeutic intervention. Here, we present 3D airway organoid platform preclinical testing active substances with regard toxicity, inflammation under standardized conditions in 96 well format. Senescence was induced doxorubicin...
Neutrophilic inflammation results in loss of lung function chronic obstructive pulmonary disease (COPD). Gram-negative bacteria, such as nontypeable Haemophilus influenzae (NTHi), trigger acute exacerbations COPD (AECOPD) and contribute to inflammation. The pro-inflammatory cytokine interleukin-17C (IL-17C) is expressed by airway epithelial cells regulates neutrophilic chemotaxis. Here, we explored the IL-17C NTHi- cigarette smoke (CS)-induced models COPD. tissue destruction were decreased...
Neutrophils contribute to lung injury in acute pneumococcal pneumonia. The interleukin 17 receptor E (IL-17RE) is the functional for epithelial-derived cytokine IL-17C, which known mediate innate immune functions. aim of this study was investigate contribution IL-17RE/IL-17C pulmonary inflammation a mouse model Streptococcus pneumoniae Numbers neutrophils and expression levels granulocyte colony-stimulating factor (G-CSF) tumor necrosis alpha (TNF-α) were decreased lungs IL-17RE-deficient...
Abstract Cystic fibrosis (CF) and chronic obstructive pulmonary disease (COPD) are associated with acute bacterial infections of the lung. Excessive differentiation basal cells to mucus-producing goblet can result in mucus hyperproduction loss mucociliary clearance airways CF COPD patients. Here, we aimed investigate effect pathogen-associated molecular patterns (PAMPs) on human 3D bronchospheres. Primary bronchial epithelial (HBECs) were differentiated bronchospheres presence flagellin LPS...
Abstract One of the major pathological hallmarks Alzheimer´s disease (AD) is an accumulation amyloid‐β (Aβ) in brain tissue leading to formation toxic oligomers and senile plaques. Under physiological conditions, a tightly balanced equilibrium between Aβ‐production ‐degradation necessary prevent Aβ‐accumulation. Here, we investigate molecular mechanism how insulin‐degrading enzyme (IDE), one Aβ‐degrading enzymes, regulated amyloid precursor protein (APP) processing Aβ‐degradation linked...
Abstract Impaired interaction of fibroblasts with pneumocytes contributes to the progression chronic lung disease such as idiopathic pulmonary fibrosis (IPF). Mucin 5B (MUC5B) is associated IPF. Here we analyzed primary and alveolar type 2 (AT2) in organoid model. Single-cell analysis, histology, qRT-PCR revealed that expressing high levels markers regulate STAT3 signaling AT2 cells, which accompanied by cystic growth MUC5B expression. Cystic expression were also caused cytokine IL-6. The...
Chronic obstructive lung disease (COPD) and cancer are both caused by smoking often occur as comorbidity. The programmed cell death protein 1/programmed ligand 1 (PD-1/PD-L1) axis is an important canonic immunoregulatory pathway, antibodies that specifically block PD-1 or PD-L1 have demonstrated efficacy therapeutic agents for non-small cancer. role of the PD-1/PD-L1 in pathogenesis COPD unknown. Here, we analyzed function preclinical models evaluated concentrations human serum...
The epithelial cytokine interleukin-17C (IL-17C) mediates inflammation through the interleukin 17 receptor E (IL-17RE). Prior studies showed a detrimental role of IL-17C in pathogenesis immune-mediated skin diseases (e.g., psoriasis). Here, we examined IL-17C/IL-17RE wound closure Staphylococcus aureus infection model. We demonstrate that is significantly delayed IL-17RE (Il-17re−/−)- and 17C (Il-17c−/−)-deficient mice. There was no significant difference between WT, Il-17re−/−, Il-17c−/−...
Abstract Background The interleukin 17 receptor E (IL-17RE) is specific for the epithelial cytokine interleukin-17C (IL-17C). Asthma exacerbations are frequently caused by viral infections. Polyinosinic:polycytidylic acid (pIC) mimics infections through binding to pattern recognition receptors (e.g. TLR-3). We and others have shown that pIC induces expression of IL-17C in airway cells. Using different mouse models, we aimed investigate function IL-17RE development experimental allergic...
Chronic lung diseases (e.g. chronic obstructive pulmonary disease, cystic fibrosis) are incurable. Epithelial senescence, a state of dysfunctional cell cycle arrest, contributes to the progression such diseases. We differntiated basal cells airway (bronchial) organoids. The organoids were treated with doxorubicin (Dox), quercetin (Quer) and combination Dox Quer. Single sequencing showed that composed (transitional) basal, secretory, goblet, deuterosomal ciliated cells. In addition, we...
Cancer research involves significant animal consumption and suffering. Tumor cells can be differentiated in vitro into three-dimensional organoids that resemble the primary tumor. In basic cancer research, however, tumor are usually only used alongside experiments. We have established an easy-to-perform protocol allows to culture KRAS-driven lung as for extended periods of time. Like corresponding tumors mice, produce surfactant protein C but no markers airway epithelial (e.g. SCGB1A1,...
Abstract Alveolar type 2 (AT2) and club cells are part of the stem cell niche lung their differentiation is required for pulmonary homeostasis tissue regeneration. A disturbed crosstalk between fibroblasts epithelial contributes to loss structure in chronic diseases. Therefore, it important understand how interact during Here we analyzed interaction alveolar epithelium modelled air-liquid interface cultures. Single-cell transcriptomics showed that co-cultivation with leads increased...
Abstract Background Infections of the respiratory tract such as pneumonia or COVID-19 cause high mortality and morbidity worldwide. Organ-on-a-chip (OC) technologies have been developed in last years to establish human-based disease models, study basic mechanisms provide a tool speed up drug development. The aim this was lung-liver microfluidic system interaction both organ modules during infection. Methods A two (lung / liver) established using primary human bronchial (HBECs) alveolar type...
Alveolar type 2 and club cells are part of the stem cell niche lung their differentiation is required for pulmonary homeostasis tissue regeneration. A disturbed crosstalk between fibroblasts epithelial contributes to loss structure in chronic diseases. Therefore, it important understand how interact during Here, we analyzed interaction alveolar epithelium modeled air-liquid interface cultures. Single-cell transcriptomics showed that cocultivation with leads increased expression markers...