A5022950451- Immune cells in cancer
- Glioma Diagnosis and Treatment
- Neuroinflammation and Neurodegeneration Mechanisms
- Phagocytosis and Immune Regulation
- Single-cell and spatial transcriptomics
- Immunotherapy and Immune Responses
- Ferroptosis and cancer prognosis
- CAR-T cell therapy research
- Prion Diseases and Protein Misfolding
- Long-Term Effects of COVID-19
- Cancer Cells and Metastasis
- Nanoplatforms for cancer theranostics
- Cancer Research and Treatments
- Cancer Immunotherapy and Biomarkers
- Cancer, Hypoxia, and Metabolism
- Extracellular vesicles in disease
- Cellular transport and secretion
- Nanoparticle-Based Drug Delivery
- Cerebrospinal fluid and hydrocephalus
- Glycosylation and Glycoproteins Research
- Protein Degradation and Inhibitors
- RNA regulation and disease
- Multiple Sclerosis Research Studies
- RNA modifications and cancer
- Hedgehog Signaling Pathway Studies
University of Basel
2017-2024
University Hospital of Basel
2014-2024
Roche Pharma AG (Germany)
2023
SIB Swiss Institute of Bioinformatics
2023
Freiwillige Akademische Gesellschaft
2023
Roche (Switzerland)
2023
University of Zurich
2007-2021
Swiss Epilepsy Center
2021
Saarland University
2021
Stanford University
2015-2019
Anti-CD47 antibody is effective for treating malignant pediatric brain tumors without detectable toxicity in patient-derived xenograft models.
Tumor-associated macrophages (TAMs) represent an important cellular subset within the glioblastoma (WHO grade IV) microenvironment and are a potential therapeutic target. TAMs display continuum of different polarization states between antitumorigenic M1 protumorigenic M2 phenotypes, with lower M1/M2 ratio correlating worse prognosis. Here, we investigated effect macrophage on anti-CD47 antibody-mediated phagocytosis human cells in vitro, as well distribution versus grown mouse xenografts....
Glioblastoma multiforme (GBM) is a highly aggressive malignant brain tumor with fatal outcome. Tumor-associated macrophages and microglia (TAMs) have been found to be major tumor-promoting immune cells in the microenvironment. Hence, modulation reeducation of tumor-associated GBM considered promising antitumor strategy. Resident invading shown distinct origin function. Whereas yolk sac-derived reside brain, blood-derived monocytes invade central nervous system only under pathological...
Growing evidence links COVID-19 with acute and long-term neurological dysfunction. However, the pathophysiological mechanisms resulting in central nervous system involvement remain unclear, posing both diagnostic therapeutic challenges. Here we show outcomes of a cross-sectional clinical study (NCT04472013) including imaging data corresponding multidimensional characterization immune mediators cerebrospinal fluid (CSF) plasma patients belonging to different Neuro-COVID severity classes. The...
Glioblastoma (GBM) is the most aggressive form of primary brain tumor, for which effective therapies are urgently needed. Cancer cells capable evading clearance by phagocytes such as microglia- and monocyte-derived through engaging tolerogenic programs. Here, we found that high expression sialic acid–binding immunoglobulin-like lectin 9 (Siglec-9) correlates with reduced survival in patients GBM. Using cell-specific knockouts Siglec-E, murine functional homolog Siglec-9, together single-cell...
Low-grade gliomas (LGGs) are rare brain neoplasms, with survival spanning up to a few decades. Thus, accurate evaluations on how biomarkers impact among patients LGG require long-term studies samples prospectively collected over long period. The 210 adult LGGs in our databank were screened for IDH1 and IDH2 mutations (IDHmut), MGMT gene promoter methylation (MGMTmet), 1p/19q loss of heterozygosity (1p19qloh), nuclear TP53 immunopositivity (TP53pos). Multivariate analyses multiple imputation...
Background: Cardiovascular diseases and chronic kidney disease (CKD) are highly prevalent, aggravate each other, account for substantial mortality. Both conditions characterized by activation of the innate immune system. The alarmin interleukin-1α (IL-1α) is expressed in a variety cell types promoting (sterile) systemic inflammation. aim present study was to examine role IL-1α mediating inflammation setting acute myocardial infarction (AMI) CKD. Methods: We assessed expression on surface...
A patient-tailored, ex vivo drug response platform for glioblastoma (GBM) would facilitate therapy planning, provide insights into treatment-induced mechanisms in the immune tumor microenvironment (iTME), and enable discovery of biomarkers response. We cultured regionally annotated GBM explants perfusion bioreactors to assess iTME responses immunotherapy. Explants were treated with anti-CD47, anti–PD-1, or their combination, analyzed by multiplexed microscopy [CO-Detection indEXing (CODEX)],...
Subgingival plaque samples were obtained from 26 subjects with advanced periodontal lesions. Bacterial diversity was analysed by amplification of the 16S rRNA genes two different primer sets, and subsequent cloning sequencing. A total 578 sequences after exclusion chimeras. The authors found 148 phylotypes clone library 27f/519r (number clones n=322; coverage, C=66 %) 75 515f/1525r (n=256; C=84 %). Comparative sequence analysis revealed that 70 % all showed a similarity at least 99 to...
Progressive accumulation of PrP(Sc), a hallmark prion diseases, occurs when conversion PrP(C) into PrP(Sc) is faster than clearance. Engulfment apoptotic bodies by phagocytes mediated Mfge8 (milk fat globule epidermal growth factor 8). In this study, we show that brain primarily produced astrocytes. ablation induced accelerated disease and reduced clearance cerebellar in vivo, as well excessive increased titers prion-infected C57BL/6 × 129Sv mice organotypic slices derived therefrom. These...
Cerebrospinal fluid leakage is an immanent risk of cranial surgery with dural opening. Recognizing the factors for this complication and improving technique closure may reduce associated morbidity its surgical burden. The aim paper was to investigate whether addition TachoSil on top suture reduces postoperative CSF compared suturing alone assess frequency potentially related complications after elective craniotomy.The authors conducted a prospective, randomized, double-blinded single-center...
Although immune checkpoint-targeted therapies are currently revolutionizing cancer care, only a minority of patients develop durable objective responses to anti-PD-1, PD-L1, and CTLA-4 therapy. Therefore, new therapeutic interventions needed increase the immunogenicity tumors overcome resistance these immunotherapies. Oncolytic properties common viruses can be exploited for priming antitumor immunity, such oncolytic in active clinical development combination with therapies. However, routine...
Immune surveillance is critical to prevent tumorigenesis. Gliomas evade immune attack, but the underlying mechanisms remain poorly understood. We show that glioma cells can sustain growth independent of system constraint by reducing Notch signaling. Loss activity in a mouse model impairs MHC-I and cytokine expression curtails recruitment anti-tumor cell populations favor immunosuppressive tumor-associated microglia/macrophages (TAMs). Depletion T simulates inhibition facilitates tumor...
Background Proteomics of glioma have not yet provided biomarkers and pathways that would clearly discriminate subgroups. Methods 82 biopsies were prospectively collected classified into six subgroups defined by methylomic classification: two Isocitrate dehydrogenase (IDH)-mutated low-grade (LGG) four high-grade (HGG) subgroups: IDH-mutated HGG (IDH HGG), proneural (GB PN), classical CL), mesenchymal glioblastoma MES). Proteins extracted processed for liquid chromatography mass spectrometry...
Prion diseases are characterized by the deposition of PrPSc, an abnormal form cellular prion protein PrPc, which is encoded Prnp gene. PrPc highly expressed on neurons and its function unknown. Recombinant was claimed to possess superoxide dismutase (SOD) activity, it hypothesized that abrogation this may contribute neurodegeneration in diseases. We tested hypothesis vivo studying copper/zinc manganese SOD activity genetically defined crosses mice lacking Sod1 gene with hemizygous or...
Prnp−/− mice lack the prion protein PrPC and are resistant to infections, but variable phenotypes have been reported in physiological function of remains poorly understood. Here we examined a cell-autonomous phenotype, inhibition macrophage phagocytosis apoptotic cells, previously mice. Using formal genetic, genomic, immunological analyses, found that regulation ascribed is instead controlled by linked locus encoding signal regulatory α (Sirpa). These findings indicate control was...
Medulloblastoma is the most common malignant brain tumor of childhood. Group 3 medulloblastoma, aggressive molecular subtype, frequently disseminates through leptomeningeal cerebral spinal fluid (CSF) spaces in and cord. The mechanism dissemination CSF remains poorly understood, pathways involved medulloblastoma metastasis self-renewal are largely unknown. Here we show that NOTCH1 signaling pathway regulates both initiation medulloblastoma. We identify a which activates BMI1 activation...
Background Growing evidence suggests that the central nervous system is affected by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), since infected patients suffer from and long-term neurological sequelae. Nevertheless, it currently unknown whether virus affects brain cortex. The purpose of this study was to assess cortical gray matter volume, thickness, surface area in a group SARS-CoV-2 with symptoms compared healthy control subjects. Additionally, we analyzed features...
Liver injury after COVID-19 vaccination is very rare and shows clinical histomorphological similarities with autoimmune hepatitis (AIH). Little known about the pathophysiology of vaccine-induced liver (VILI) its relationship to AIH. Therefore, we compared VILI AIH.Formalin-fixed paraffin-embedded biopsy samples from patients (n = 6) an initial diagnosis AIH 9) were included. Both cohorts by evaluation, whole-transcriptome spatial transcriptome sequencing, multiplex immunofluorescence, immune...