A5075100407- Bone Metabolism and Diseases
- TGF-β signaling in diseases
- Heterotopic Ossification and Related Conditions
- Bone health and treatments
- Muscle Physiology and Disorders
- Skin and Cellular Biology Research
- Parathyroid Disorders and Treatments
- NF-κB Signaling Pathways
- Cellular Mechanics and Interactions
- Oral and Maxillofacial Pathology
- Adipose Tissue and Metabolism
- Axon Guidance and Neuronal Signaling
- Bone and Joint Diseases
- Oral and gingival health research
- Biochemical Analysis and Sensing Techniques
- Tissue Engineering and Regenerative Medicine
- Polysaccharides and Plant Cell Walls
- Orthopaedic implants and arthroplasty
- Bone Tissue Engineering Materials
- Nutrition and Health in Aging
- Medical and Biological Sciences
- Oral Health Pathology and Treatment
- Mesenchymal stem cell research
- Regulation of Appetite and Obesity
- Endodontics and Root Canal Treatments
Kyushu Dental University
2016-2025
Federation of American Societies for Experimental Biology
2023
Saitama Medical University
2009-2018
Harvard University
2011-2017
Smad1, Smad5 and Smad9 (also known as Smad8) are activated by phosphorylation bone morphogenetic protein (BMP)-bound type I receptor kinases. We examined the role of creating constitutively active forms (SmadDVD). Transcriptional activity Smad9DVD was lower than that Smad1DVD or Smad5DVD, even though all three SmadDVDs associated with Smad4 bound to target DNA. The linker region sufficient reduce transcriptional activity. expression increased activation BMP signaling, similar inhibitory...
Enhancing bone morphogenetic protein (BMP) signaling increases formation in a variety of settings that target repair. However, the role BMP maintenance adult mass is not well understood. Targeted disruption BMP3 mice results increased trabecular formation, whereas transgenic overexpression skeletal cells leads to spontaneous fracture, consistent with having negative regulation. Here we investigate importance as mediator skeleton. We find osteoblasts (OBL) and osteocytes are source bone....
Recent studies have indicated that acetylcholine (ACh) plays a vital role in various tissues, while the of ACh bone metabolism remains unclear. Here we demonstrated induced cell proliferation and reduced alkaline phosphatase (ALP) activity via nicotinic (nAChRs) muscarinic receptors (mAChRs) osteoblasts. We detected mRNA expression several nAChRs mAChRs. Furthermore, showed cholinergic components were up‐regulated subunits/subtypes altered during osteoblast differentiation. To our knowledge,...
The taste system extends beyond the oral cavity, with various receptors found in extraoral organs. Mice deficient receptor type 1 (TAS1R) family member, TAS1R3, and fed a high-fat, high-sugar diet showed high bone mass without altering food consumption. However, underlying mechanisms, including cell types responsible for TAS1R3 expression, remain unclear. Here, we demonstrate expression function of osteoclasts, which are resorption. Tas1r3 but not Tas1r1 or Tas1r2, is evoked during...
Bone morphogenic proteins (BMPs) stimulate bone formation in vivo and osteoblast differentiation vitro via a Smad signaling pathway. Recent findings revealed that the activation of nuclear factor-κB (NF-κB) inhibits BMP-induced differentiation. Here, we show NF-κB BMP by directly targeting A selective inhibitor classic pathway, BAY11–770682, enhanced BMP2-induced ectopic vivo. In mouse embryonic fibroblasts (MEFs) prepared from mice deficient p65, main subunit NF-κB, BMP2, induced...
The world’s population is aging. Pneumonia the leading cause of death among older adults, with aspiration pneumonia being particularly common. Aspiration caused by a decline in swallowing function. Causes can include age-related sarcopenia muscles, cognitive decline, cerebrovascular and other diseases or even changes individual taste preference. Currently, main treatment approach for dysphagia resistance training swallowing-related muscles. This has not been effective establishment novel...
Abstract Phosphorylation of Smad1/5/8 at carboxyl-terminal serine residues by type I receptors activates downstream bone morphogenetic protein (BMP) signaling. Protein phosphatase magnesium-dependent 1A (PPM1A) has been shown to suppress BMP activity dephosphorylating phospho-Smads. We report here that PPM1A suppresses signaling via a novel mechanism. inhibited constitutively activated Smad1 mutant lacking receptor phosphorylation sites. reduced the levels not only but also Smad5 and Smad8....
Bone is a highly vascularized organ, thus angiogenesis vital process during bone remodeling. However, the role of vascular systems in remodeling not well recognized. Here we show that netrin‐4 inhibits osteoclast differentiation vitro and vivo. Co‐cultures marrow macrophages with endothelial cells markedly inhibited differentiation. Adding neutralizing antibody, or RNA interference against netrin‐4, restored Administration prevented loss an osteoporosis mouse model by decreasing number. We...
Honeybees produce royal jelly (RJ) from their cephalic glands. Royal is a source of nutrition for the queen honey bee throughout its lifespan and also involved in fertility longevity. has long been considered beneficial to human health. We recently observed that RJ delayed impairment motor function during aging, affecting muscle fiber size. However, how affects skeletal metabolism functional component as yet unidentified. demonstrate feeding mice with daily prevents decrease myofiber size...
DNA methylation is an epigenetic modification critical for the regulation of chromatin structure and gene expression during development disease. The ten-eleven translocation (TET) enzyme family catalyzes hydroxymethylation subsequent demethylation by oxidizing 5-methylcytosine (5mC) to 5-hydroxymethylcytosine (5hmC). Little known about TET protein function due a lack pharmacological tools manipulate levels. In this study, we examined role TET-mediated BMP-induced C2C12 osteoblast...
Bone morphogenetic proteins (BMPs) induce osteoblastic differentiation in myogenic cells via the phosphorylation of Smads. Two types Smad phosphatases--small C-terminal domain phosphatase 1 (SCP1) and protein magnesium-dependent 1A--have been shown to inhibit BMP activity. Here, we report that SCP1 inhibits induced by BMP-4, a constitutively active receptor, form Smad1. The activity was required for this suppression, knockdown myoblasts stimulated signaling. In contrast 1A, did not reduce...