A5019894815- Bone Metabolism and Diseases
- NF-κB Signaling Pathways
- TGF-β signaling in diseases
- Endodontics and Root Canal Treatments
- Bone and Dental Protein Studies
- Oral microbiology and periodontitis research
- Immune Response and Inflammation
- Dental Radiography and Imaging
- Orthopaedic implants and arthroplasty
- Bone health and treatments
- Dental materials and restorations
- Chemokine receptors and signaling
- Retinoids in leukemia and cellular processes
- Oral and Maxillofacial Pathology
- Inflammatory mediators and NSAID effects
- RNA Research and Splicing
- interferon and immune responses
- RNA modifications and cancer
- Platelet Disorders and Treatments
- Cancer-related molecular mechanisms research
- RNA Interference and Gene Delivery
- Cancer-related gene regulation
- Wound Healing and Treatments
- Dental Research and COVID-19
- RNA regulation and disease
Hiroshima University
2018-2024
Kyushu Dental University
2014-2020
St. Marianna University School of Medicine
2016
Bone morphogenic proteins (BMPs) stimulate bone formation in vivo and osteoblast differentiation vitro via a Smad signaling pathway. Recent findings revealed that the activation of nuclear factor-κB (NF-κB) inhibits BMP-induced differentiation. Here, we show NF-κB BMP by directly targeting A selective inhibitor classic pathway, BAY11–770682, enhanced BMP2-induced ectopic vivo. In mouse embryonic fibroblasts (MEFs) prepared from mice deficient p65, main subunit NF-κB, BMP2, induced...
Bone morphogenetic protein (BMP) potentiates bone formation through the Smad signaling pathway in vitro and vivo. The transcription factor nuclear κB (NF-κB) suppresses BMP-induced osteoblast differentiation. Recently, we identified that transactivation (TA) 2 domain of p65, a main subunit NF-κB, interacts with mad homology (MH) 1 Smad4 to inhibit BMP signaling. Therefore, further attempted identify interacting regions these two molecules at amino acid level. We region term Smad4-binding...
The periodontal ligament is a soft connective tissue embedded between the alveolar bone and cementum, surface hard of teeth. Periodontal fibroblasts (PDLF) actively express osteo/cementogenic genes, which contribute to homeostasis. However, key factors maintaining abilities PDLF remain unclear. We herein demonstrated that PPARγ was expressed by in vivo its distribution pattern correlated with alkaline phosphate enzyme activity. knockdown markedly reduced vitro, whereas agonists exerted...
To investigate whether glycosaminoglycans (GAGs) binding to high-dose LL37 eliminates its cytotoxicity dental pulp cells (hDPCs) whilst retaining undiminished antimicrobial and LPS-neutralizing abilities.hDPCs were stimulated with varying concentrations of LL37, their cell viability was analysed by MTT. Then, (10 μmol L-1 ) bound three GAGs, heparin, chondroitin sulphate hyaluronic acid, cytotoxic effects on hDPCs evaluated compared. Furthermore, the ability heparin (5 μg mL-1 )-LL37...
Enforced enrichment of the active promoter marks trimethylation histone H3 lysine 4 (H3K4me3) and acetylation 27 (H3K27ac) by inhibiting demethylases deacetylases is positively associated with hard tissue formation through induction osteo/odontogenic differentiation. However, key endogenous epigenetic modulator odontoblasts to regulate expression genes coding dentin extracellular matrix (ECM) proteins has not been identified. We focused on nuclear factor (NF)–κB inhibitor ζ (IκBζ), which was...
Abstract It is well known that dental pulp tissue can evoke some of the most severe acute inflammation observed in human body. We found cells secrete a factor induces tumor necrosis factor-α production from macrophages, and designated this factor, cell-derived powerful inducer TNF-α (DPIT). DPIT was induced transported to recipient via microvesicles. Treatment with PKR inhibitor markedly suppressed activity, weak interferon signals were constitutively activated inside cells. In stimulation...
Peroxisome proliferator-activated receptor γ (PPARγ) is a major transcription factor of energy metabolism-associated genes, and three PPARγ isoforms have been identified in periodontal tissues cells. When metabolism homeostasis affected by downregulation ligament fibroblasts (PDLFs), osteo/cementogenic abilities are markedly lost. Herein, we investigated whether agonists promote tissue regeneration, which metabolic pathways indispensable for abilities. A agonist was locally administered to...
Dental pulp stem cells (DPSC) usually remain quiescent in the dental tissue; however, once tissue is injured, DPSCs potently proliferate and migrate into injury microenvironment contribute to immuno-modulation repair. However, key molecules that physiologically support potent proliferation migration of have not been revealed. In this study, we searched publicly available transcriptome raw data sets, which contain comparable (i.e., equivalently cultured) DPSC mesenchymal cell data. Three sets...
The purpose of the present study was to compare clinical efficacy between a flowable-type nano-hybrid composite and paste-type for posterior restoration.Of 62 teeth in 33 patients (mean age: 34.1 years), 31 were filled with (Heliomolar [HM] group), another flowable (MI FIL [MI] group). Clinical evaluated at 2 years after restoration.There no differences retention, surface texture deterioration, anatomical form change, deterioration marginal adaptation, secondary caries, while statistical...
Regulation of inflammation is important for pulp wound healing, including protective responses by odontoblast-like cells. However, methods directly regulating have not yet been described. The inflammatory response mediated a transcription factor, nuclear factor-κB (NF-κB), which activates cytokines tumor necrosis factor (TNF)-α. Macromolecular translocation inhibitor II (MTI-II) was previously demonstrated as an enhancer the transcriptional activity glucocorticoid-bound glucocorticoid...
Nuclear factor-κB (NF-κB) is the most potent proinflammatory transactivator, and an inhibitor of NF-κB a good antiinflammatory drug. Glucocorticoids (GCs) are strongest frequently used drugs. GC-bound glucocorticoid receptor (GR) inhibits transcriptional activity thereby suppresses broad range inflammatory processes. Concurrently, in whole body outside inflammation area, GR exerts lot hormone action, which results severe side effects. There long-awaited need for new inhibitor. Previously we...
The spread of root canal infection to surrounding periodontal tissue through accessory canals reduces the success rate endodontic treatment. In this case, cone-beam computed tomography revealed a lesion (4 mm from apex) resulting an maxillary left central incisor. First, non-surgical treatment was conducted but sinus tract remained. Surgical preparation cavity then remove potentially infected dentin canal. filled and foramen sealed with resin containing bioactive surface pre-reacted glass...
To investigate the effect of increasing apical size roots enlarged for root canal obturation on outcome non-surgical endodontic treatment teeth with periodontitis.
This study aimed to elucidate the pathogenesis of idiopathic gingival fibromatosis (IGF). Human fibroblasts (hGFs) were isolated from patients with IGF and periodontitis. Differential gene expression in hGFs was analyzed using RNA sequencing. Extracellular matrix-related analyzed. The effect specific protein (SP)1 inhibitor or recombinant human transglutaminase 2 (rh-TGM2) on biglycan (BGN) also determined. sequencing showed that TGM2 downregulated BGN mRNA upregulated relative rh-TGM2...