A5025826172- HIV Research and Treatment
- HIV/AIDS Research and Interventions
- HIV/AIDS drug development and treatment
- Viral Infections and Immunology Research
- Genomics and Phylogenetic Studies
- Metal complexes synthesis and properties
- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Hepatitis B Virus Studies
- Genetic Associations and Epidemiology
- Synthesis and Biological Evaluation
- Carbohydrate Chemistry and Synthesis
- Hepatitis C virus research
- Respiratory viral infections research
- Fungal Biology and Applications
- Viral gastroenteritis research and epidemiology
- Transgenic Plants and Applications
- Ferrocene Chemistry and Applications
- Genomics and Rare Diseases
- Click Chemistry and Applications
- DNA and Nucleic Acid Chemistry
University of Botswana
2012-2022
The hepatitis B virus (HBV) is a global problem; however, the burden of HBV infection in pregnant women Botswana unknown. We sought to determine prevalence chronic and occult human immunodeficiency (HIV)-infected -uninfected Botswana. Samples from 752 were tested for surface antigen (HBsAg), HBsAg-positive samples e (HBeAg) DNA load. that HBsAg negative screened by determining genotypes determined based on 415-base-pair fragment gene. Among during pregnancy or early postpartum, 16 (2.1%)...
The potential antimicrobial properties of a tridentate polypyridyl ligand 4-butoxy- N , -bis(pyridin-2-ylmethyl)aniline (BUT) 1 and its corresponding mixed ruthenium complexes were investigated on drug-resistant non-drug-resistant bacterial species. synthesized successfully characterized by H NMR, UV/Vis, FTIR spectra; ESI-MS; magnetic susceptibility. Electronic spectra susceptibility these Ru(II)/(III) suggest that they are low spin crystal field split, where the Ru(III) is d 5 Ru(II) 6...
Background: Individuals living with human immunodeficiency virus (HIV) who experience virological failure (VF) after combination antiretroviral therapy (cART) initiation may have had low-frequency drug resistance mutations (DRMs) at cART initiation. There are no data on DRMs among cART-naïve HIV-positive individuals in Botswana. Methods: We evaluated the prevalence of previously sequenced using Sanger sequencing. The generated pol amplicons were by next-generation Results: observed (detected...
A new hexadentate 2-picolyl-polypyridyl-based ligand (4, 4'-(butane-1, 4-diylbis(oxy))bis(N, N-bis(pyridin-2-ylmethyl)aniline)) (2BUT) (1) and its corresponding Ru(II/III) complexes were synthesized characterized, followed by assessment of their possible bioactive properties towards drug-resistant non-drug-resistant bacteria. Spectroscopic characterization the was done using proton NMR, FTIR, ESI-MS, which showed that successfully synthesized. The characterized UV/Vis, elemental analysis,...
<ns4:p><ns4:bold>Background:</ns4:bold>HIV-1 drug resistance poses a major threat to the success of antiretroviral therapy. The high costs available HIV assays prohibit their routine usage in resource-limited settings. Pan-degenerate amplification and adaptation (PANDAA), focused genotyping approach based on quantitative PCR (qPCR), promises fast cost-effective way detect mutations (HIVDRMs). Given cost current methods, we sought use PANDAA for screening key HIVDRMs antiretroviral-naïve...
<ns4:p><ns4:bold>Background:</ns4:bold>HIV-1 drug resistance poses a major threat to the success of antiretroviral therapy. The high costs available HIV assays prohibit their routine usage in resource-limited settings. Pan-degenerate amplification and adaptation (PANDAA), focused genotyping approach based on quantitative PCR (qPCR), promises fast cost-effective way detect mutations (HIVDRMs). Given cost current methods, we sought use PANDAA for screening key HIVDRMs antiretroviral-naïve...
ABSTRACT The study of human genome variations can contribute towards understanding population diversity and the genetic aetiology health-related traits. We sought to characterise genomic Botswana in order assess elucidate mutation burden using whole sequencing. Whole sequences 390 unrelated individuals from were available for computational analysis. mapped reference GRCh38. Population joint variant calling was performed Genome Analysis Tool Kit (GATK) BCFTools. Variant characterisation...
ABSTRACT Despite the high burden of HIV-1 in Botswana, population Botswana is significantly underrepresentation host genetics studies HIV-1. Furthermore, bulk previous genomics evaluated common human genetic variations, however, there increasing evidence influence rare variants outcome diseases which may be uncovered by comprehensive complete and deep genome sequencing. This research aimed to evaluate role rare-variants susceptibility progression through whole Whole sequences (WGS) 265...