A5024618487- Extracellular vesicles in disease
- Microtubule and mitosis dynamics
- Cellular Mechanics and Interactions
- Fungal and yeast genetics research
- Genetic Neurodegenerative Diseases
- Cellular transport and secretion
- Peptidase Inhibition and Analysis
- Hereditary Neurological Disorders
- Mitochondrial Function and Pathology
- Ubiquitin and proteasome pathways
- Reproductive biology and impacts on aquatic species
- Protease and Inhibitor Mechanisms
- Fish Ecology and Management Studies
- Cell Adhesion Molecules Research
- Micro and Nano Robotics
- MicroRNA in disease regulation
- Protein Degradation and Inhibitors
- Plant nutrient uptake and metabolism
- Sphingolipid Metabolism and Signaling
- Cardiomyopathy and Myosin Studies
- Protist diversity and phylogeny
- Adenosine and Purinergic Signaling
- Wnt/β-catenin signaling in development and cancer
- Endoplasmic Reticulum Stress and Disease
- Histone Deacetylase Inhibitors Research
Xavier University of Louisiana
2014-2024
Columbia University
2002-2022
University of California, San Francisco
2010
Using FM4-64 to label endosomes and Abp1p-GFP or Sac6p-GFP actin patches, we find that (1) colocalize with patches as they assemble at the bud cortex; (2) undergo linear, retrograde movement from buds toward mother cells; (3) interact disassemble FM4-64–labeled internal compartments. We also show flow of cables mediates patch movement. An Arp2/3 complex mutation decreases frequency cortical, nonlinear movements, but has no effect on velocity Rather, linear occurs same direction cables....
Retrograde flow of cortical actin networks and bundles is essential for cell motility retrograde intracellular movement, the formation maintenance microvilli, stereocilia, filopodia. Actin cables, which are F-actin that serve as tracks anterograde cargo movement in budding yeast, undergo driven, part, by polymerization assembly. We find cable rate reduced deletion or delocalization type II myosin Myo1p, conditional mutation Myo1p motor domain. Deletion tropomyosin isoform Tpm2p, but not...
In migrating cells, external signals polarize the microtubule (MT) cytoskeleton by stimulating formation of oriented, stabilized MTs and inducing reorientation MT organizing center (MTOC). Glycogen synthase kinase 3beta (GSK3beta) has been implicated in each these processes, although whether it regulates both processes a single system how its activity is regulated are unclear. We examined issues wound-edge, serum-starved NIH 3T3 fibroblasts where stabilization MTOC triggered lysophosphatidic...
Drosophila Kinesin-73 (Khc-73), which plays a role in mitotic spindle polarity neuroblasts, is metazoan-specific member of the Kinesin-3 family motors, includes mammalian KIF1A and Caenorhabditis elegans Unc-104. The mechanism motors has been controversial because some studies have reported that they transport cargo as monomers whereas other suggested dimer mechanism. Here, we performed single-molecule motility cell biological Khc-73. We find constructs containing motor conserved short...
Exosomes are small extracellular microvesicles that secreted by cells when intracellular multivesicular bodies fuse with the plasma membrane. We have previously demonstrated Nischarin inhibits focal adhesion formation, cell migration, and invasion, leading to reduced activation of kinase. In this study, we propose tumor suppressor regulates release exosomes. When cocultured on exosomes from Nischarin-positive cells, breast cancer exhibited survival, adhesion, spreading. The same cocultures...
Kif5A is a neuronally-enriched isoform of the Kinesin-1 family cellular transport motors. 23 separate mutations in motor domain have been identified patients with complicated form hereditary spastic paraplegia (HSP). We performed vitro assays on dimeric recombinant HSP-causing Switch I domain, which participates coordination and hydrolysis ATP by kinesin. observed variety significantly reduced catalytic mechanical activities as result each mutation, shared phenotype from that motility was...
Abstract In yeast, actin cables are F-actin bundles that essential for cell division through their function as tracks cargo movement from mother to daughter cell. Actin also affect yeast lifespan by promoting transport and inheritance of higher-functioning mitochondria cells. Here, we report cable stability declines with age. Our genome-wide screen genes identified the open reading frame YKL075C . Deletion results in increases abundance, mitochondrial fitness, replicative lifespan....
Purpose: The cell surface LDL (low-density lipoprotein) receptor-related protein-1 (LRP-1) is important for lipid transport and several signaling processes. Human apolipoprotein E (apoE) a ligand of LRP-1. We previously reported that short peptide (apoEdp) mimicking the LRP-1 binding region apoE prevents hyperglycemia-induced retinal endothelial dysfunction in vitro. in-vivo outcome apoE-based peptidomimetic inhibition treatment diabetic retinopathy unknown.Methods: Six months after...
Follicle cell processes (FCP) are identified in two species of carcharhinid shark (Selachii) but absent the little skate Leucoraja erinacea (Batoidea). This suggests that FCPs either a unique structure evolved selachians or were lost by batoids after their divergence, some 280 mya. The presence would be consistent with evolution large oocytes this group animals.
The development of therapeutic resistance in the majority patients limits long-term benefit ROS1 inhibitor treatment. On-target mutations kinase domain confer to crizotinib and lorlatinib more than one-third acquired cases with no current effective treatment option. As an alternative stoichiometric inhibition, proteolytic degradation could provide tool combat generated by these mutations. Our study has identified a potent, orally active degrader excellent pharmacokinetics profile. can...
Lysine deacetylases (KDACs or HDACs) are metal‐dependent enzymes that regulate lysine acetylation, a post‐translational modification is present on thousands of human proteins, essential for many cellular processes, and often misregulated in diseases. The selective inhibition KDACs would allow understanding the biological roles individual therapeutic targeting enzymes. Recent studies have suggested purportedly specific KDAC inhibitors significant off‐target binding, but consequences binding...
Abstract In yeast, actin cables are F-actin bundles that essential for cell division through their function as tracks cargo movement from mother to daughter cell. Actin also affect yeast lifespan by promoting transport and inheritance of higher-functioning mitochondria cells. Here, we report cable stability declines with age. Our genome-wide screen genes identified the open reading frame YKL075C . Deletion results in increases abundance, mitochondrial fitness, replicative lifespan....
Abstract Follicle cell processes (FCP) are actin‐based, tube‐like structures that connect the developing oocyte to follicle cells throughout oogenesis. They were first described in Selachians (sharks) where their suggested roles facilitating transport of metabolites and providing structural support large egg sharks, an early stage evolution viviparity. Subsequent studies found FCP absent Rajiformes (skates), suggesting may have been novel specific sharks. Here, Hydrolagus colliei , a...
<div>Abstract<p>Exosomes are small extracellular microvesicles that secreted by cells when intracellular multivesicular bodies fuse with the plasma membrane. We have previously demonstrated Nischarin inhibits focal adhesion formation, cell migration, and invasion, leading to reduced activation of kinase. In this study, we propose tumor suppressor regulates release exosomes. When cocultured on exosomes from Nischarin-positive cells, breast cancer exhibited survival, adhesion,...
<p>Exosomes from Nischarin Tumors Reduce Focal Adhesions and Cell Spreading</p>
<p>Caspase 3 Staining of Mouse Tumors From Exosome Studies</p>
<p>Nischarin Decreases the Attachment of Breast Cancer Cells</p>
<p>Schematic Representation of the Effects Nischarin on Breast Cancer Cell Motility through Exosomes</p>