A5084314895- Biofuel production and bioconversion
- Natural product bioactivities and synthesis
- Microbial Metabolic Engineering and Bioproduction
- Plant biochemistry and biosynthesis
- Enzyme Production and Characterization
- Plant Gene Expression Analysis
- Phytochemical compounds biological activities
- Enzyme Catalysis and Immobilization
- Microbial Metabolites in Food Biotechnology
- Ginseng Biological Effects and Applications
- Pharmacological Effects of Natural Compounds
- Polysaccharides and Plant Cell Walls
- Phytochemicals and Antioxidant Activities
- Medicinal Plant Pharmacodynamics Research
- Enzyme-mediated dye degradation
- Microbial Metabolism and Applications
- Photosynthetic Processes and Mechanisms
- Flavonoids in Medical Research
- Catalysis for Biomass Conversion
- Glycosylation and Glycoproteins Research
- Fungal Biology and Applications
- Chromatography in Natural Products
- Studies on Chitinases and Chitosanases
- Biochemical and biochemical processes
- Advanced Cellulose Research Studies
Nanjing Forestry University
2016-2025
Institute of Chemical Industry of Forest Products
2016-2025
Jiangsu University
2014
Jiangnan University
2009-2011
Nanjing Normal University
2005-2010
Liaoning Provincial People's Hospital
2010
β-Glucosidase is an important component of the cellulase enzyme system. It does not only participate in cellulose degradation, it also plays role hydrolyzing to fermentable glucose by relieving inhibition exoglucanase and endoglucanase from cellobiose. Therefore, glucose-tolerant β-glucosidase with high specific activity for cellobiose might be a potent candidate industrial applications.The gene bgl that encodes 443-amino-acid protein was cloned over-expressed Thermoanaerobacterium...
Hyperoside exhibits many biological properties and is more soluble in water than quercetin. A uridine 5′-diphosphate (UDP) galactose regeneration system one-pot synthesis of hyperoside was described herein. Glycine max sucrose synthase (GmSUS) coupled with Escherichia coli UDP-galactose 4-epimerase (GalE) to regenerate from UDP. Petunia hybrida glycosyltransferase (PhUGT) high activity toward quercetin used synthesize via the system. The important factors for optimal synergistic catalysis...
Astragalin (kaempferol 3-O-glucoside) is used as a standard to assess the quality of Radix astragali and has exhibited number biological properties. In this work, we screened several UDP-dependent glycosyltransferases (UGT) for their potential efficient biocatalysts astragalin synthesis. The highest production with 285 mg/L was detected in recombinant strain expressing UGT from Arabidopis thaliana (AtUGT78D2). To further improve production, an UDP-glucose synthesis pathway reconstructed by...
Dihydro-β-ionone is a common type of ionone used in the flavor and fragrance industries because its characteristic scent. The production flavors microbial cell factories offers sustainable environmentally friendly approach to accessing them, independent extraction from natural sources. However, native pathway dihydro-β-ionone remains unclear, hindering heterologous biosynthesis hosts. Herein, we devised platform for de novo syntheses simple carbon source with glycerol. complete was...
The 1,914-bp open reading frame of xylC from Thermoanaerobacterium saccharolyticum JW/SL-YS485 encodes a calculated 73-kDa β-xylosidase, XylC, different any glycosyl hydrolase in the database and representing novel glycohydrolase family. Hydrolysis occurred under retention anomeric configuration, transglycosylation presence alcohols as acceptors. With use vector pHsh, expression third β-xylosidase this bacterium, increased approximately 4-fold when loop within translational initiation region...
Thermally stable α-L-rhamnosidase with cleaving terminal α-L-rhamnose activity has great potential in industrial application. Therefore, it is necessary to find a proper method improve the thermal stability of α-L-rhamnosidase. In this study, addition sorbitol been found increase thermostability from Aspergillus terreus at temperatures ranging 65 °C 80 °C. Half-life and activation free energy 2.0 M 70 were increased by 17.2-fold, 8.2 kJ/mol, respectively. The analyses results fluorescence...
β-D-xylosidase is a vital exoglycosidase with the ability to hydrolyze xylooligosaccharides xylose and biotransform some saponins by cleaving outer β-xylose. widely used as one of xylanolytic enzymes in diverse range applications, such fuel, food pharmaceutical industry; therefore, more studies have focused on thermostable xylose-tolerant β-D-xylosidases. A β-xylosidase gene (xln-DT) 1509 bp was cloned from Dictyoglomus thermophilum expressed E.coli BL21. According amino acid phylogeny...
Isoorientin is a C-glycosylated derivative of luteolin and exhibits number biological properties. In this study, multiple strategies were adopted to improve isoorientin production from in Escherichia coli. was improved substantially by adjusting induction controlling acetic acid accumulation, with maximum reaching 826 mg/L. Additionally, novel UDP-glucose synthesis pathway reconstructed E. coli through cellobiose phosphorylase-catalyzed phosphorolysis for the glucose 1-phosphate, which...
Cellulose, as the most abundant and cheap renewable resource in nature, is of great importance for its utilization. An enzymatic cellulose solution, mainly containing cellobiose glucose, was utilized to produce astragalin instead recombinant strains. However, crystalline structure affects production cellobiose, resulting a low yield. In this study, microcrystalline pretreated with phosphoric acid CaCl
Fisetin is mainly extracted from Rhus succedanea L., but it also found in a variety of foods, vegetables, and herbs, commonly used dietary supplements for its health benefits. However, limited stability, low oral bioavailability, poor absorption hinder developmental applications. Methylation modification has emerged as an effective strategy to enhance the solubility, lipid solubility fisetin. In this study, we identified novel 3'-O-methyltransferase, PeCCoAOMT, characterized enzymatic...
The complete degradation of the cellulose requires synergistic action endo-β-glucanase, exo-β-glucanase, and β-glucosidase. But endo-β-glucanase exo-β-glucanase can be recovered by solid–liquid separation in hydrolysis their binding domain (CBD), however, β-glucosidases cannot because most without CBD, so additional are necessary for next degradation. This will increase cost glucose-tolerant β-glucosidase (BGL) from Thermoanaerobacterium thermosaccharolyticum DSM 571 was fused with (CBD)...
(i) To investigate the enzymatic characterization of α-L-arabinofuranosidase from Thermotoga thermarum DSM5069. (ii) evaluate performance its excellent properties on converting ginsenoside Rc to Rd.The thermostable (Tt-Afs) gene T. DSM5069 was cloned and overexpressed. Recombinant Tt-Afs purified, molecular weight approx. 55 kDa. Its optimal activity at pH 5·0 95°C. It has high selectivity for cleaving outer arabinofuranosyl moieties C-20 carbon sugar-tolerance makes a promising candidate...