A5016570491- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Antibiotic Resistance in Bacteria
- Forensic and Genetic Research
- Genomics and Phylogenetic Studies
- Monoclonal and Polyclonal Antibodies Research
- Urinary Bladder and Prostate Research
- Pharmaceutical Practices and Patient Outcomes
- Bioactive Compounds and Antitumor Agents
- CRISPR and Genetic Engineering
- Marine Bivalve and Aquaculture Studies
- Attention Deficit Hyperactivity Disorder
- Protist diversity and phylogeny
- Paleontology and Stratigraphy of Fossils
- Forensic Anthropology and Bioarchaeology Studies
- Bacterial Genetics and Biotechnology
- Diabetes Management and Research
- Cancer therapeutics and mechanisms
- Plant biochemistry and biosynthesis
- Marine Biology and Ecology Research
- Radiation Effects and Dosimetry
- Environmental DNA in Biodiversity Studies
- Fossil Insects in Amber
- African Studies and Geopolitics
- Economic and Financial Impacts of Cancer
AstraZeneca (Netherlands)
2024
Triangle
2023
AstraZeneca (Brazil)
2022
Wright State University
2012-2018
Dayton Children's Hospital
2017-2018
Manchester University
2018
University of Brighton
2018
SUNY Brockport
2017
University of Cape Town
1990-2015
John Innes Centre
2004-2012
Journal Article Rooting molecular trees: problems and strategies Get access ANDREW B. SMITH 1Department of Palaeontology, The Natural History Museum, Cromwell Road, London SW7 5BD, U.K Search for other works by this author on: Oxford Academic Google Scholar Biological the Linnean Society, Volume 51, Issue 3, March 1994, Pages 279–292, https://doi.org/10.1111/j.1095-8312.1994.tb00962.x Published: 14 January 2008 history Received: 04 August 1992 Accepted: 15 1993
Novobiocin is a member of the coumermycin family antibiotics and well-established inhibitor DNA gyrase. Recent studies have shown that novobiocin binds to previously unrecognized ATP-binding site at C-terminus Hsp90 induces degradation Hsp90-dependent client proteins ∼700 μM. In an effort develop more efficacious inhibitors C-terminal binding site, library analogues was prepared initial structure−activity relationships revealed. These data suggested 4-hydroxy moiety coumarin ring...
Apparently ancient DNA has been reported from amber–preserved insects many millions of years old. Rigorous attempts to reproduce these sequences amber– and copal–preserved bees flies have failed detect any authentic insect DNA. Lack reproducibility suggests that does not survive over even in amber, the most promising fossil environments.
Echinoderms have a unique ontogeny and adult structure, and, among Bilateria, are the phylum that has diverged most radically in appearance from ancestral body plan. Embryology gene expression studies suggest how this transformation may occurred while paleontological data provide direct evidence for order which these events took place. Comparing echinoderm genetic developmental signalling patterns with those of their sister group, hemichordates, suggests an evolutionary switch posterior...
ABSTRACT Despite improvements in volumetric titer for monoclonal antibody (MAb) production processes using Chinese hamster ovary (CHO) cells, some “difficult‐to‐express” (DTE) MAbs inexplicably reach much lower process titers. These DTE require intensive cell line and development activity, rendering them more costly or even unsuitable to manufacture. To rapidly rationally identify an optimal strategy improve of MAbs, we have developed engineering design platform combining high‐yielding...
Xanthomonas albilineans produces a family of polyketide-peptide compounds called albicidins which are highly potent antibiotics and phytotoxins as result their inhibition prokaryotic DNA replication. Here we show that albicidin is inhibitor the supercoiling activity bacterial plant gyrases, with 50% inhibitory concentrations (40 to 50 nM) less than those most coumarins quinolones. Albicidin blocks religation cleaved intermediate during gyrase catalytic sequence also inhibits relaxation...
The oldest contemporary human mitochondrial lineages arose in Africa. earliest divergent extant maternal offshoot, namely haplogroup L0d, is represented by click-speaking forager peoples of southern Broadly defined as Khoesan, Khoesan are today largely restricted to the semidesert regions Namibia and Botswana, whereas archeological, historical, genetic evidence promotes a once broader southerly dispersal including southward migrating pastoralists indigenous marine-foragers. No data have been...
Abstract- 18S ribosomal RNA sequences from 11 echinoderms are analysed using parsimony to investigate phylogenetic relationships. Their estimated divergence limes range less than 20 Ma more 550 before present. Phylogenies based on rRNA sequence data compared with well-established morphological phylogenies discover at what evolutionary distance the two approaches start produce incongruent results. Three regions of molecule separately and together, paired unpaired sites also treated combined....
The oldest extant human maternal lineages include mitochondrial haplogroups L0d and L0k found in the southern African click-speaking forager peoples broadly classified as Khoesan. Profiling these early allows for better understanding of modern evolution. In this study, we profile 77 new early-diverged complete genomes sub-classify another 105 L0d/L0k individuals from Africa. We use data to refine basal phylogenetic divergence, coalescence times Khoesan prehistory. Our results confirm...
Abstract Omicron and its subvariants have steadily gained greater capability of immune escape compared to other variants concern, resulting in an increased incidence reinfections even among vaccinated individuals. We evaluated the antibody response BA.1, BA.2, BA.4/5 US military members with primary 2-dose series Moderna mRNA-1273 a cross-sectional study. While nearly all participants had sustained spike (S) IgG neutralizing antibodies (ND50) ancestral strain, only 7.7% detectable ND50 BA.1...
DNA gyrase is the only topoisomerase able to introduce negative supercoils into DNA. Absent in humans, a successful target for antibacterial drugs. However, increasing drug resistance serious problem and new agents are urgently needed. The naturally-produced Escherichia coli toxin CcdB has been shown by what predicted be novel mechanism. previously stabilize 'cleavage complex', but it not inhibit catalytic reactions of gyrase. We present data showing that does indeed stabilization cleavage...
Despite the development of high-titer bioprocesses capable producing >10 g L−1 recombinant monoclonal antibody (MAb), some so called "difficult-to-express" (DTE) MAbs only reach much lower process titers. For widely utilized "platform" processes discrete variable is protein coding sequence product. However, there has been little systematic study to identify parameters that affect expression. This information vital, as it would allow us rationally design genetic and engineering strategies for...
"Distribution and Prevalence of Spotted Fever Group Rickettsiae in Ticks from South Carolina, with an Epidemiological Survey Persons Bitten by Infected Ticks" published on Nov 1978 The American Society Tropical Medicine Hygiene.
Bacterial toxin-antitoxin (TA) systems encode two proteins, a potent inhibitor of cell proliferation (toxin) and its specific antidote (antitoxin). Structural data has revealed striking similarities between the model TA toxins CcdB, DNA gyrase encoded by ccd system plasmid F, Kid, site-specific endoribonuclease parD R1. While common structural fold seemed at odds with clearly different modes action these toxins, possibility functional crosstalk systems, which would further point to their...
Biopharmaceutical manufacturing using Chinese hamster ovary (CHO) cells requires the generation of high-producing clonal cell lines. During line development, cloning fluorescence-activated sorting (FACS) has potential to combine isolation single with based on specific cellular attributes that correlate productivity and/or growth, identifying lines desirable phenotypes for manufacturing. This study describes application imaging flow cytometry (IFC) characterize recombinant at single-cell...
DNA ligases are essential enzymes in cells due to their ability join strand breaks formed during replication. Several temperature-sensitive mutant strains of Escherichia coli, including strain GR501, have been described which can be complemented by functional ligases. Here, it is shown that the ligA251 mutation E. coli GR501 a cytosine thymine transition at base 43, results substitution leucine phenylalanine residue 15. The protein product this gene (LigA251) accumulated similar level...
Aggregation of therapeutic bispecific antibodies negatively affects the yield, shelf-life, efficacy and safety these products. Pairs stable Chinese hamster ovary (CHO) cell lines produced two difficult-to-express with different levels aggregated product (10-75% aggregate) in a miniaturised bioreactor system. Here, transcriptome analysis was used to interpret biological causes for aggregation identify strategies improve yield quality. Differential expression- gene set revealed upregulated...
In constructing phylogenies from molecular data both the composition of ingroup and choice outgroup can strongly affect chances obtaining correct topology. This is because tree topology uneven rates evolution ability tree-building algorithms to find tree. Outgroups be added in one two ways, either a single sister clade (preferably closest) or through addition taxa different clades. On theoretical empirical grounds former strategy shown much more beneficial, terms redressing balance reducing...