A5047586892- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Protein purification and stability
- Advanced Proteomics Techniques and Applications
- Transgenic Plants and Applications
- Advanced Fluorescence Microscopy Techniques
- Glycosylation and Glycoproteins Research
- Bacteriophages and microbial interactions
- Advanced Biosensing Techniques and Applications
- Cellular transport and secretion
- Lysosomal Storage Disorders Research
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Radiopharmaceutical Chemistry and Applications
- Complement system in diseases
- Nanofabrication and Lithography Techniques
- Pancreatic function and diabetes
- HER2/EGFR in Cancer Research
- vaccines and immunoinformatics approaches
- Virology and Viral Diseases
- Innovative Microfluidic and Catalytic Techniques Innovation
- Nanoparticle-Based Drug Delivery
- SARS-CoV-2 and COVID-19 Research
- Machine Learning in Bioinformatics
- Bioinformatics and Genomic Networks
KTH Royal Institute of Technology
2016-2024
AlbaNova
2018-2021
A database provides information on the relative abundances and locations of human proteins that are predicted to be secreted.
Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify metabolic engineering targets limiting expression human proteins through a systems biology analysis transcriptomes CHO and HEK293 during expression. In an comparison 24 express proteins, one third challenging displayed improved secretion upon host cell swapping from HEK293. Guided by comprehensive transcriptomics between lines,...
The proteins secreted by human tissues and blood cells, the secretome, are important both for basic understanding of biology identification potential targets future diagnosis therapy. Here, a high-throughput mammalian cell factory is presented that was established to create resource recombinant full-length covering majority those annotated as 'secreted' in humans. DNA sequences each predicted were generated gene synthesis, constructs transfected into Chinese hamster ovary (CHO) cells...
Abstract The complement component 5 (C5)-binding antibody eculizumab is used to treat patients with paroxysmal nocturnal hemoglobinuria (PNH) and atypical haemolytic uremic syndrome (aHUS). As recently reported there a need for precise classification of responsive allow safe cost-effective treatment. To such stratification, knowledge the binding site drug on its target crucial. Using structural epitope mapping strategy based bacterial surface display, flow cytometric sorting validation via...
Abstract The proteins secreted by human tissues (the secretome) are important for the basic understanding of biology, but also identification potential targets future diagnosis and therapy. Here, we present an annotation all predicted (n=2,623) with information about their spatial distribution in body. A high-throughput mammalian cell factory was established to create a resource recombinant full-length proteins. This used phenotypic assays involving β-cell dedifferentiation development...
Monoclonal antibodies (mAbs) have proved to be a valuable tool for the treatment of different cancer types. However, clinical use an increasing number mAbs, also highlighted limitations with monotherapy cancers, in particular such more complex mechanisms, requiring action on additional molecules or pathways, cancers quickly acquiring resistance following monotherapy. An example latter is mAb trastuzumab, FDA approved metastatic gastric carcinoma. To circumvent this, researchers reported...
Abstract Complementation-dependent fluorescence is a powerful way to study co-localization or interactions between biomolecules. A split-GFP variant, involving the self-associating GFP 1–10 and 11, has previously provided convenient approach measure recombinant protein titers in cell supernatants. limitation of this slow chromophore formation after complementation. Here, we alleviate lag signal generation by allowing mature on solid support containing 11 before applying analyses. The...
Aggregation of therapeutic bispecific antibodies negatively affects the yield, shelf-life, efficacy and safety these products. Pairs stable Chinese hamster ovary (CHO) cell lines produced two difficult-to-express with different levels aggregated product (10-75% aggregate) in a miniaturised bioreactor system. Here, transcriptome analysis was used to interpret biological causes for aggregation identify strategies improve yield quality. Differential expression- gene set revealed upregulated...
Abstract Precise epitope determination of therapeutic antibodies is great value as it allows for further comprehension mechanism action, responsiveness prediction, avoidance unwanted cross reactivity, and vaccine design. The golden standard discontinuous the laborious X-ray crystallography method. Here, we present a combinatorial method rapid mapping epitopes by mammalian antigen display, eliminating need protein expression purification. facilitated automated workflows tailored software...
Rare diseases are, despite their name, collectively common and millions of people are affected daily conditions where treatment often is unavailable. Sulfatases a large family activating enzymes related to several these diseases. Heritable genetic variations in sulfatases may lead impaired activity reduced macromolecular breakdown within the lysosome, with severe lethal as consequence. While therapeutic options scarce, for some sulfatase deficiencies by recombinant enzyme replacement...
Summary Biologics represent the fastest growing group of therapeutics, but many advanced recombinant protein moieties remain difficult to produce. Here, we identify bottlenecks limiting expression human proteins through a systems biology analysis transcriptomes CHO and HEK293 during overexpression. Surprisingly, one third challenging displayed improved secretion upon host cell swapping from HEK293. While most components secretory machinery showed comparable levels in both hosts, genes with...