A5044186093- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Genomics and Chromatin Dynamics
- Innovations in Medical Education
- Neuroscience and Neuropharmacology Research
- Occupational Health and Safety Research
- Neurogenesis and neuroplasticity mechanisms
- Infection Control in Healthcare
- MicroRNA in disease regulation
- Schizophrenia research and treatment
- RNA Research and Splicing
- Bioinformatics and Genomic Networks
- Neuroscience and Neural Engineering
- COVID-19 epidemiological studies
- Quality and Safety in Healthcare
- Cancer-related gene regulation
- Tryptophan and brain disorders
- Gene expression and cancer classification
- Growth Hormone and Insulin-like Growth Factors
- Medical Education and Admissions
- Thyroid Disorders and Treatments
- Autism Spectrum Disorder Research
National University of Singapore
2014-2024
National University Health System
2009-2020
National University Hospital
2020
Agency for Science, Technology and Research
2010-2017
Singapore Institute for Clinical Sciences
2009-2017
RIKEN Center for Brain Science
2007-2015
Imperial College London
2011
Faculty of Public Health
2011
Development Fund
2011
Kanazawa University
2004-2007
Abstract To understand the molecular actions of status epilepticus at chromatin level, we studied effects kainate‐induced on two different histone modifications amino terminal tails: H3 phosphorylation serine 10 and H4 acetylation. In addition to induction c‐ fos jun immediate early genes (IEGs) expression in mouse hippocampus, also found upregulation acetylation histones, coupled with after kainate administration. mRNA were sequentially induced response kainate, hippocampal subpopulations,...
Despite its prominence for characterization of complex mixtures, LC-MS/MS frequently fails to identify many proteins. Network-based analysis methods, based on protein-protein interaction networks (PPINs), biological pathways, and protein complexes, are useful recovering non-detected proteins, thereby enhancing analytical resolution. However, network-based methods do come in varied flavors which the respective efficacies largely unknown. We compare recovery performance functional insights...
Abstract Clinical symptoms may vary and not necessarily reflect serum thyroid hormone (TH) levels during acute chronic hyperthyroidism as well recovery from hyperthyroidism. We thus examined changes in hepatic gene expression TH/TSH adult male mice treated either with a single T3 (20 μg per 100 g body weight) injection (acute T3) or daily injections for 14 days (chronic followed by 10 of withdrawal. Gene arrays livers harvested at these time points showed that among positively-regulated...
The capacity for plasticity in the adult brain is limited by anatomical traces laid down during early postnatal life. Removing certain molecular brakes, such as histone deacetylases (HDACs), has proven to be effective recapitulating juvenile mature visual cortex (V1). We investigated chromatin structure and transcriptional control genome-wide sequencing of DNase I hypersensitive sites (DHSS) cap analysis gene expression (CAGE) libraries after HDAC inhibition valproic acid (VPA) V1. found...
On March 12, 2003, the World Health Organization issued a global health alert stating that new, unrecognizable, flulike disease may spread to care workers (HCWs). We now know this illness as severe acute respiratory syndrome (SARS). By August there were 8422 SARS cases and 916 deaths reported from 29 countries. galvanized world threat of emerging infectious diseases provided dress rehearsal for subsequent challenges such H5N1 H1N1 influenza. Among insights gained following: reminded us work...
Although experience-dependent changes in brain inhibitory circuits are thought to play a key role during the "critical period" of development, nature and timing these poorly understood. We examined sensory experience sculpting an circuit primary somatosensory cortex (S1) mice by using optogenetics map connections between parvalbumin (PV) expressing interneurons layer 2/3 pyramidal cells. Unilateral whisker deprivation decreased strength spatial range input provided neurons PV layers 2/3, 4...
Nida F. Degesys, MD; Ralph C. Wang, MD, MAS; Elizabeth Kwan, Jahan Fahimi, MPH; Jeanne A. Noble, MA; Maria Raven, MPH
During early postnatal development, neuronal circuits are sculpted by sensory experience provided the external environment. This experience-dependent regulation of circuitry development consolidates balance excitatory-inhibitory (E/I) neurons in brain. The cortical barrel-column that innervates a single principal whisker is used to provide clear reference frame for studying consolidation E/I circuitry. Sensory deprivation S1 at birth disrupts decreasing inhibitory transmission parvalbumin...
Structured training for the prevention of needlestick injuries (NSIs) among medical students was implemented in Singapore 1998. In this study, we determined incidence NSIs and knowledge practice managing reporting first-year clinical a school Singapore, as well adequacy provided these students, 14 years after preventive instituted.All third-year (n = 257) from Yong Loo Lin School Medicine, National University who had completed their first year posting were enrolled cross-sectional study. A...
Abstract The brain adapts to dynamic environmental conditions by altering its epigenetic state, thereby influencing neuronal transcriptional programs. An example of an modification is protein methylation, catalyzed arginine methyltransferases (PRMT). One member, Prmt8, selectively expressed in the central nervous system during a crucial phase early development, but little else known regarding function. We hypothesize Prmt8 plays role synaptic maturation development. To evaluate this, we used...
Abstract Summary: microRibonucleic acid (miRNAs) are small regulatory molecules that act by mRNA degradation or via translational repression. Although many miRNAs ubiquitously expressed, a subset have differential expression patterns may give rise to tissue-specific complexes. Motivation: This work studies gene targeting amongst with profiles, and links this control regulation of protein Results: We find that, when pair not expressed in the same tissues, there is higher tendency for them...
Abstract In order to elucidate mechanisms underlying modulation by static magnetism of the cellular functionality and/or integrity in brain, we screened genes responsive brief cultured rat hippocampal neurons using differential display analysis. We have for first time cloned and identified Ntan1 (amidohydrolase N‐terminal asparagine) as a gene brain. is an essential component protein degradation signal, which destabilizing residue protein, N‐end rule. situ hybridization histochemistry...
Abstract With coronavirus disease 2019 declared a Public Health Emergency of International Concern on 30 January 2020, occupational health services in tertiary hospital Singapore stepped up via three-pronged approach, namely, protection individual staff, staff workforce, and prevention nosocomial spread so as to support business continuity plans. Despite the multiple new challenges brought by COVID-19 pandemic, hospital’s were able adapt keep all employees patients safe with strong from...
We investigated the effects of environmental enrichment during critical period early postnatal life and how it interplays with epigenome to affect experience-dependent visual cortical plasticity. Mice raised in an EE from birth CP have increased spine density dendritic complexity cortex. upregulates synaptic plasticity genes, Arc Egr1, a transcription factor MEF2C. also observed increase MEF2C binding promoters Egr1. In addition, pups show reduction HDAC5 its Mef2c, Egr1 genes. With...
Valproic acid (VPA) is an anti-convulsant drug that recently shown to have neuroregenerative therapeutic actions. In this study, we investigate the underlying molecular mechanism of VPA and its effects on Bdnf transcription through microRNAs (miRNAs) their corresponding target proteins. Using in silico algorithms, predicted from our miRNA microarray iTRAQ data miR-124 likely at guanine nucleotide binding protein alpha inhibitor 1 (GNAI1), adenylate cyclase inhibitor. With reduction GNAI1...
The essential role of the Reelin gene (RELN) during brain development makes it a prominent candidate in human epigenetic studies Schizophrenia. Previous literature has reported differing levels DNA methylation (DNAm) patients with psychosis. Therefore, this study aimed to (1) examine and compare RELN DNAm subjects at different stages psychosis cross-sectionally, (2) analyse effect antipsychotics (AP) on DNAm, (3) evaluate effectiveness applicability promoter as possible biological-based...