A5045710186- Congenital heart defects research
- Pancreatic function and diabetes
- CRISPR and Genetic Engineering
- Gene Regulatory Network Analysis
- Pluripotent Stem Cells Research
- Cardiomyopathy and Myosin Studies
- Chemotherapy-induced cardiotoxicity and mitigation
- Lung Cancer Treatments and Mutations
- Zebrafish Biomedical Research Applications
- Growth Hormone and Insulin-like Growth Factors
- 3D Printing in Biomedical Research
- Glycosylation and Glycoproteins Research
- Metabolism, Diabetes, and Cancer
- Single-cell and spatial transcriptomics
- RNA Interference and Gene Delivery
- Genomics and Chromatin Dynamics
- Lysosomal Storage Disorders Research
- Microfluidic and Bio-sensing Technologies
- Genetics and Neurodevelopmental Disorders
- Nitric Oxide and Endothelin Effects
- Cardiac Ischemia and Reperfusion
- Ion Transport and Channel Regulation
- Virus-based gene therapy research
- Cancer, Hypoxia, and Metabolism
- Diabetes and associated disorders
Shenzhen Second People's Hospital
2024
Shenzhen Institutes of Advanced Technology
2024
Southern University of Science and Technology
2014-2021
National Center for Advancing Translational Sciences
2018-2021
National Institutes of Health
2018-2021
University of North Carolina at Chapel Hill
2008-2021
Shanghai Jiao Tong University
2021
Ruijin Hospital
2006-2021
Zhejiang Provincial People's Hospital
2007-2021
Women's Hospital, School of Medicine, Zhejiang University
2021
A synthetic circuit implementing density-controlled bacterial motility autonomously produces a tunable stripe pattern.
Rationale: Although a cardioprotective function of target rapamycin (TOR) signaling inhibition has been suggested by pharmacological studies using rapamycin, genetic evidences are still lacking. We explored adult zebrafish as novel vertebrate model for dissecting pathways in cardiomyopathy. Objective: generated the second cardiomyopathy induced doxorubicin. By genetically analyzing both doxorubicin and our previous established anemia-induced models, we decipher functions TOR cardiomyopathies...
Background Anthracycline chemotherapeutics, such as doxorubicin, are used widely in the treatment of numerous malignancies. The primary dose-limiting adverse effect anthracyclines is cardiotoxicity that often presents heart failure due to dilated cardiomyopathy years after anthracycline exposure. Recent data from animal studies indicate cause cardiac atrophy. timing onset and underlying mechanisms not well defined, relevance these findings human disease unclear. Methods Results Wild-type...
AimsMutations in the essential myosin light chain (ELC) and regulatory (RLC) genes have been linked to sarcomeric hypertrophic cardiomyopathies humans; however, specific functions of different chains during cardiogenesis a vertebrate animal are not well understood.
Pancreatic β-cells are an essential source of insulin and their destruction because autoimmunity causes type I diabetes. We conducted a chemical screen to identify compounds that would induce the differentiation insulin-producing in vivo. To do this screen, we brought together use transgenic zebrafish as model β-cell differentiation, unique multiwell plate allows easy visualization lateral views swimming larval fish library clinical drugs. identified six hits can precocious secondary islets...
Diabetes is associated with a paucity of insulin-producing β-cells. With the goal finding therapeutic routes to treat diabetes, we aim find molecular and cellular mechanisms involved in β-cell neogenesis regeneration. To facilitate discovery such mechanisms, use vertebrate organism where pancreatic cells readily regenerate. The larval zebrafish pancreas contains Notch-responsive progenitors that during development give rise adult ductal, endocrine, centroacinar (CACs). Adult CACs are also...
Background An adult zebrafish heart possesses a high capacity of regeneration. However, it has been unclear whether and how myocyte hyperplasia contributes to cardiac remodeling in response biomechanical stress hypertrophy exists the zebrafish. To address these questions, we characterized mutant tr265/tr265, whose Band 3 mutation disrupts erythrocyte formation results anemia. Although does not express function heart, chronic anemia imposes sequential towards heart. Methodology/Principal...
The genetic study of titin has been notoriously difficult because its size and complicated alternative splicing routes. Here, we have used zebrafish as an animal model to investigate the functions individual isoforms. We identified 2 orthologs in zebrafish, ttna ttnb, annotated full-length genomic sequences for both genes. found that ttna, but not is required sarcomere assembly heart well subsequent establishment cardiac contractility. In fact, earliest sarcomeric mRNA expressed heart, which...
Mutagenesis screening is a powerful genetic tool for probing biological mechanisms underlying vertebrate development and human diseases. However, the increased colony management efforts in vertebrates impose significant challenge identifying genes affecting particular organ, such as heart, especially those exhibiting adult phenotypes on depletion.We aim to develop facile approach that streamlines via enriching cardiac mutants, which enables us screen phenotypes.The transparency of zebrafish...
Alpha-1 adrenergic receptors (α1-ARs) play adaptive and protective roles in the heart. Dabuzalgron is an oral selective α1A-AR agonist that was well tolerated multiple clinical trials of treatment for urinary incontinence, but has never been used to treat heart disease humans or animal models. In this study, authors administered dabuzalgron mice treated with doxorubicin (DOX), a widely chemotherapeutic agent dose-limiting cardiotoxicity can lead failure (HF). protected against DOX-induced...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTHighly active position eight analogs of somatostatin and separation peptide diastereomers by partition chromatographyChester A. Meyers, David H. Coy, W. Y. Huang, Andrew V. Schally, Tommie ReddingCite this: Biochemistry 1978, 17, 12, 2326–2331Publication Date (Print):June 13, 1978Publication History Published online1 May 2002Published inissue 13 June...
The pure trophoblastic cell lines BeWo and Jar derived from human ch oriocarcinomas produced estrone estradiol tritiated androstenedione dehydroepiandrosterone. Typically, 8 million cells were incubated at 37 degrees C for 10 hours in ml meduim containing the appropriate labeled substrate. Products isolated by reverse phase partition chromatography, purified recrystallation to constant specific activity formation of acetate derivatives. In 1 experiment line 2120 ng 14,200 per g day...
Most novel cancer therapeutics target kinases that are essential to tumor survival. Some of these kinase inhibitors associated with cardiotoxicity, whereas others appear be cardiosafe. The basis for this distinction is unclear, as the molecular effects in heart.
Infantile and late infantile neuronal ceroid lipofuscinoses (NCLs) are lysosomal storage diseases affecting the central nervous system (CNS). The NCL (INCL) is caused by mutations in PPT1 gene late-infantile (LINCL) due to TPP1 gene. Deficiency or enzyme function results accumulation of pathological lipofuscin-like material patient cells. There currently no small-molecular drug treatment for NCLs. We have generated induced pluripotent stem cells (iPSC) from three dermal fibroblast lines...
The nuclear receptor retinoic acid-related orphan receptor-α (RORα) regulates numerous critical biological processes, including central nervous system development, lymphocyte differentiation, and lipid metabolism. RORα has been recently identified in the heart, but very little is known about its role cardiac physiology. We sought to determine whether myocardial hypertrophy cardiomyocyte survival context of angiotensin II (ANG II) stimulation. For vivo characterization function pathological...
The human kinome consists of roughly 500 kinases, including 150 that have been proposed as therapeutic targets. Protein kinases regulate an array signalling pathways control metabolism, cell cycle progression, death, differentiation and survival. It is not surprising, then, new kinase inhibitors developed to treat cancer, sorafenib, also exhibit cardiotoxicity. We hypothesized sorafenib cardiotoxicity related its deleterious effects on specific cardiac metabolic given the critical roles...
Screening drug combinations using a high-throughput and open-space DOE microfluidic chip can play vital role in the development of disease treatment personalized medicine.
Abstract Cytokines have attracted sustained attention due to their multi-functional cellular response in immunotherapy. However, application was limited short half-time, narrow therapeutic window, and undesired side effects. To address this issue, we developed a portable smart blue-light controlled (PSLC) device based on optogenetic technology. By combining PSLC with gene modules, successfully achieved the targeted regulation of cytokine expression within tumor microenvironment. alter...