A5008466560- Lipid Membrane Structure and Behavior
- Polymer Surface Interaction Studies
- Protein Structure and Dynamics
- Protein purification and stability
- Spacecraft and Cryogenic Technologies
- Antimicrobial Peptides and Activities
- Supramolecular Self-Assembly in Materials
- Alzheimer's disease research and treatments
- Protein Interaction Studies and Fluorescence Analysis
- Parkinson's Disease Mechanisms and Treatments
- Biochemical and Structural Characterization
- Surfactants and Colloidal Systems
- Methane Hydrates and Related Phenomena
- Electrostatics and Colloid Interactions
- DNA and Nucleic Acid Chemistry
- Ion-surface interactions and analysis
- Surface and Thin Film Phenomena
- biodegradable polymer synthesis and properties
- Nuclear Structure and Function
- Metabolomics and Mass Spectrometry Studies
- Copper-based nanomaterials and applications
- Inorganic Fluorides and Related Compounds
- Calcium Carbonate Crystallization and Inhibition
- Nanopore and Nanochannel Transport Studies
- Spectroscopy and Quantum Chemical Studies
TU Dortmund University
2014-2019
Institut Laue-Langevin
2016-2017
Islet amyloid polypeptide (IAPP) is responsible for cell depletion in the pancreatic islets of Langherans, and multiple pathological consequences encountered by patients suffering from type 2 Diabetes Mellitus. We have examined amyloidogenicity cytotoxic mechanisms this peptide investigating model-membrane permeation structural effects fragments human IAPP several rat mutants. In vitro experiments molecular dynamics simulations reveal distinct physical segregation, membrane permeation,...
The outer membrane (OM) of Gram-negative bacteria is a complex and asymmetric bilayer that antimicrobial peptides must disrupt in order to provoke the cell lysis. inner external leaflets OM are mainly composed phospholipids (PL), lipopolysaccharide (LPS), respectively. Supported lipid bilayers interesting model systems mimic scaffold determine quantitative mechanistic effect agents, using complementary physicochemical techniques. We report formation PL/LPS Langmuir-Blodgett/Langmuir-Schaefer...
We investigated the volumetric and kinetic profile of conformational landscape a poly dA loop DNA hairpin (Hp) in presence salts, osmolytes crowding media, mimicking intracellular milieu, using single-molecule FRET methodology. Pressure modulation was applied to explore hydrational characteristics free-energy Hp, but also because pressure is stress factor many organisms have cope with, e.g. deep sea where pressures even up kbar level are encountered. Urea synergistically destabilize closed...
The heat-induced desorption and adsorption of the proteins lysozyme, ribonuclease A, bovine serum albumin, fibronectin at protein layers was investigated in two different environments: pure buffer solution. Using environments allows us to distinguish between thermodynamic kinetic mechanisms process. We observed a an solution, depending upon properties, such as size, stability, charge. conclude that is mainly influenced by mobility interface, while solution driven conformational changes and,...
The deep-sea osmolyte TMAO does not only stabilize proteins against high pressure, it affects also the fluidity and lateral organization of membranes.
Iron oxide monolayers are grown on Ag(0 0 1) via reactive molecular beam epitaxy (metal deposition in oxygen atmosphere). The monolayer shows FeO stoichiometry as concluded from x-ray photoemission spectra. Both low energy electron diffraction well scanning tunneling microscopy demonstrate that the layer has a quasi-hexagonal (1 1 structure although deposited surface with square symmetry. Compared to bulk values, FeO(1 is unidirectionally expanded by 3.4% directions while values maintained...
Poly(ethylene glycol) (PEG) brushes are reputed for their ability to prevent undesired protein adsorption material surfaces exposed biological fluids. Here, out of human blood serum onto PEG anchored solid-supported lipid monolayers was characterized by neutron reflectometry, yielding volume fraction profiles headgroups, PEG, and adsorbed proteins at subnanometer resolution. For both PEGylated non-PEGylated surfaces, adsorb as a thin layer approximately 10 Å, overlapping with the headgroup...
Large aggregates of misfolded α-synuclein inside neuronal cells are the hallmarks Parkinson's disease. The protein's natural function and its supposed toxicity, however, believed to be closely related interaction with cell vesicle membranes. Upon this interaction, protein folds into an α-helical structure intercalates membrane. In study, we focus on changes in lipid bilayer caused by intrusion. situ X-ray reflectivity was applied determine vertical density before after exposure α-synuclein....
We present an in situ X-ray reflectivity study of the adsorption behavior protein lysozyme on titanium oxide layers under variation different thermodynamic parameters, such as temperature, hydrostatic pressure, and pH value. Moreover, by varying layer thickness a silicon wafer, changes were studied. In total, we determined less compared with dioxide, while increasing causes stronger adsorption. Furthermore, temperature from 20 to 80 °C yields increase amount adsorbed at interface. Additional...
An X-ray reflectivity study on the adsorption behavior of human apolipoprotein A1 (apoA1) at hydrophilic and hydrophobic interfaces is presented. It shown that protein interacts via electrostatic interactions with interfaces, resulting in absorption protein. pH dependent measurements solid/liquid interface between silicon dioxide aqueous solution show a small range 4 6, increased due to attraction. Here, native shape seems be conserved. In contrast, liquid/gas mainly driven by effects,...
We studied the adsorption behavior of gas octafluoropropane at water/gas interface as a function different pressures. In custom-made measurement cell, pressure was varied in range between 1 bar and close to condensation octafluoropropane. The electron density profiles layers show that layer thickness increases with pressure. evolution indicates bulk is reached if consisting more than one monolayer adsorbed on water surface.
Protein adsorption at interfaces is of great relevance in industry and medicine. The growth biofilms strongly influenced by adsorbed proteins. control this biofilm formation necessary for technical medical applications. Fundamental mechanisms protein solid/liquidand liquid/gas-interfaces are investigated surface-sensitive x-ray scattering methods. proteins lysozyme, ribonuclease A, bovine serum albumin, fibronectin apolipoprotein A1. temperature-induced desorption the solid/liquidinterface...