Monitoring plays a significant role in improving the quality of service cloud computing. It helps clouds to scale resource utilization adaptively, identify defects services for developers, and discover usage patterns numerous end users. However, due heterogeneity components complexity arising from wealth runtime information, monitoring faces many new challenges. In this paper, we propose model (RMCM), which denotes an intuitive representation running by focusing on common concerns. Raw data...

Trichophyton species, the leading cause of dermatophytosis globally, are increasingly resistant to antifungal treatments, concerns about effective management strategies. In light absence established resistance criteria for terbinafine and azoles, coupled with a dearth research on mechanisms in Trichophyton, susceptibility drug gene diversity were analyzed across 64 T. mentagrophytes, 65 interdigitale, 2 indotineae isolates collected China between 2001 2024 101 published strains. Analyses...

CD8+ T cells are crucial to establish antitumor immunity, and their high infiltration associates with favorable prognoses. However, several cell subpopulations in the tumor microenvironment may play different roles prognosis, progression, immunotherapy. Here, we analyzed prior published single-cell RNA-sequencing (scRNA-seq) melanoma data explore heterogeneity of identified 7 major subpopulations. We found that exhausted subpopulation 2 would contribute unfavorable In contrast, a large...

Web service technology aims to enable the interoperation of heterogeneous systems and reuse distributed functions in an unprecedented scale has achieved significant success. There are still, however, challenges realize its full potential. One these is ensure behavior services consistent with their requirements. Monitoring events that relevant requirements is, thus, important technique. This paper introduces online monitoring approach for It includes a pattern-based specification constraints...

This study aimed to validate the effectiveness of matrix-assisted laser desorption ionization-time flight mass spectrometry (MALDI-TOF MS)-based identification filamentous fungi order Mucorales. A total 111 isolates covering six genera preserved at Research Center for Medical Mycology Peking University were selected MALDI-TOF MS analysis. We emphasized 23 strains

Cloud computing, a paradigm shift that offer computing resources in pay-as-you-go manner, is expected to minimize service operators' cost without sacrificing the performance of services. However, it much harder guarantee cloud applications due complex architecture cloud, interaction between co-deployed and unpredictability workload. Some research work has reported existed not performing as satisfactorily expected. How stable turns into major concern both providers providers. In this paper,...

Immune checkpoints play various important roles in tumour immunity, which usually contribute to T cells' exhaustion, leading immunosuppression the microenvironment. However, of immune infectious diseases, especially fungal infection, remain elusive. Here, we reanalyzed a recent published single-cell RNA-sequencing (scRNA-seq) data peripheral blood mononuclear cells (PBMCs) stimulated with Candida albicans (C. albicans), explore expression patterns after C. bloodstream infection. We...

Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) has emerged as a powerful microorganism identification tool. Research on MALDI-TOF MS rare filamentous fungi is still lacking. This study aimed to evaluate the performance in Scedosporium, Acremonium-like, Scopulariopsis, and Microascus species. Sabouraud broth cultivation formic acid/acetonitrile protein extraction were used for by Bruker Biotyper system. An in-house database containing 29 isolates...

Abstract Common histopathologic techniques are used to diagnose fungal infections, but the diagnostic identification of mycoses in tissue specimens is often difficult, particularly when fungi rarely occur a specimen. The aim this study was evaluate application fluorescein-labeled chitinase staining formalin-fixed and paraffin-embedded (FFPE) tissues. We studied 79 archival FFPE tissues from patients diagnosed with disease, including 38 cases sporotrichosis 41 other infections. sections were...

Intra-abdominal candidiasis (IAC) is the predominant type of invasive with high mortality in critically ill patients. This study aimed to investigate whether polymerase chain reaction (PCR) assay for detecting Candida DNA peritoneal fluids (PF) useful diagnosing and management IAC high-risk patients intensive care unit (ICU). A prospective single-center cohort surgical at risk was conducted ICU. PF collected from abdominal drainage tubes (within 24 h) or by percutaneous puncture. Direct...

Platform as a Service (PaaS) is typical cloud service paradigm that allows PaaS consumers to deploy and manage applications (usually services SaaS consumers). To ensure the quality of both consumers, must be equipped with enough monitoring controlling ability make runtime adjustment actions. Although most components in have provided their own management interface, it hard perform actions based on raw data collected from these low level interfaces due diversity dynamics PaaS. This paper...

This study aimed to detect the identification limitations for Aspergillus species from patients or environment based on MALDI-TOF MS analysis. A total of 209 isolates were selected in this study. One hundred and sixty-eight strains as challenge analysis, while remaining 41 used construct a supplementary database. The 168 identified by Bruker Filamentous Fungi Library v1.0 (the Library) again using combined with database database). sensitivity alone identifying at level was 64.3 85.7%,...

SaaS (Software as a Service) has been widely recognized new kind of service. Meanwhile, PaaS (Platform is emerging in recent years. The management service on platform quite different from that traditional services. This paper analyzed the activities and proposed an intuitive easy approach to such deploying, monitoring controlling. All these can be taken by developers themselves. Based approach, flexible scalable self-management framework implemented. key characteristics are using static...

Abstract Background and objectives Matrix‐assisted laser desorption ionisation‐time‐of‐flight mass spectrometry (MALDI‐TOF MS) is a novel technique for identifying dermatophytes. This study aimed to detect the limitation of MALDI‐TOF MS applied Methods A total 113 DNA‐sequenced dermatophyte isolates preserved at Research Center Medical Mycology Peking University were selected this study. Forty‐two as reference strains used create supplementary database. Seventy‐one ( Trichophyton rubrum...

ABSTRACT Objectives Tinea capitis remains a common fungal infection in children worldwide. Species identification is critical for determining the source of and reducing transmission. In conventional methods, macro‐ microscopic analysis time‐consuming results slow growth or low specificity. We propose rapid real‐time diagnostic PCR method that allows species‐specific dermatophytes, including Microsporum canis complex, Trichophyton mentagrophytes rubrum complex tonsurans, patients with tinea...

As an effective approach to maintain software system without interrupting the service, online migration has been applied for many goals, e.g. achieving a higher performance, tolerating faults, and upgrading systems. In this paper, we propose middleware-based application migration. Independent from operating system, middleware not only provides information required perform migration, but also offers flexible efficient way We implement our on J2EE platform, one of most important platforms in...

With the on-demand service provision ability and elastic nature, cloud computing provides a suitable development running environment for internetware, to support its cooperation evolvement. Among three models of computing, Platform-as-a-Service (PaaS) internetware application developers with sufficient convenient resources develop run their applications. As other models, monitoring is basis scalability in PaaS. Apart from this, also required that should be provided PaaS consumers. However,...

Application Programming Interface (API) constraints on objects are rules that API client code must follow in order to get expected results from these objects. Runtime verification, an important approach for detecting constraint violations, usually suffers high runtime overhead. This paper focuses temporal multiple interacting Violation detection of such is more challenging than violation single object constraints, and may induce higher To reduce the overhead, without compromising...

The Amount of e-learning resources available in the internet or local corpora has increased while our ability to search and retrive relevant information is being reduced. So distributed integration significant for enforcement novel searching mechnisms e-learing repositories. Our apparoach introduces an advanced learning organization framework mechanism which initializes a semantic model representing digital content stored servers through use ontologies. In this way, tearing materials are...