A5020068777- Monoclonal and Polyclonal Antibodies Research
- T-cell and B-cell Immunology
- Cell Adhesion Molecules Research
- Immune Cell Function and Interaction
- Organ Donation and Transplantation
- Erythrocyte Function and Pathophysiology
- Blood groups and transfusion
- Immunotherapy and Immune Responses
- Renal and Vascular Pathologies
- Rheumatoid Arthritis Research and Therapies
- French Urban and Social Studies
- Immune Response and Inflammation
- Glycosylation and Glycoproteins Research
- Viral Infections and Immunology Research
- Blood properties and coagulation
- Virus-based gene therapy research
- CAR-T cell therapy research
- Organ Transplantation Techniques and Outcomes
- Social Sciences and Governance
- Multiculturalism, Politics, Migration, Gender
- Protease and Inhibitor Mechanisms
- Systemic Lupus Erythematosus Research
- Chronic Kidney Disease and Diabetes
- Autoimmune and Inflammatory Disorders Research
- Health, Medicine and Society
McGill University
2021-2022
Université de Sherbrooke
2022
International Association for the Study of Pain
2022
Assistance Publique – Hôpitaux de Paris
2010-2020
Hôpital Necker-Enfants Malades
1983-2019
Université Paris Cité
1998-2017
Psychologie : Interactions, Temps, Emotions, Cognition
2009-2012
Inserm
1999-2010
Institut Cochin
1994-2010
Centre National de la Recherche Scientifique
1990-2010
Gene expression analysis of target organs might help provide new insights into the pathogenesis autoimmune diseases. We used global gene profiling minor salivary glands to identify patterns in patients with primary Sjögren's syndrome (pSS), a common and prototypic systemic disease. allowed for differentiating most pSS from controls. The 23 genes IFN pathways, including two Toll-like receptors (TLR8 TLR9), was significantly different between Furthermore, increased IFN-inducible genes, BAFF...
Collagen-induced arthritis (CIA), an animal model for rheumatoid arthritis, is induced in DBA/1 (H-2q) mice following immunization with type II collagen (CII) CFA. Since we have previously shown that IFN-gamma exerts a biphasic effect during the evolution of CIA mice, analyzed development this disease disruption receptor gene (IFN-gammaR(0/0)). Mutant were interbred strain to yield IFN-gammaR(0/0) expressing H-2q haplotype. In three consecutive experiments, male found exhibit severe clinical...
Abstract Interferon‐γ (IFN‐γ) exerts both enhancing and suppressing influences on collagen‐induced arthritis (CIA), depending the route protocol of administration. To study role IFN‐γ autoimmune process CIA, we treated DBA/1 mice with two different rat monoclonal antibodies (mAb) to murine IFN‐γ. Treatments, given twice weekly for 4 weeks, consisted intraperitoneal injections either mAb. In early treatments, starting from day immunization type II collagen (CII), severity was reduced in...
The anti-inflammatory effects of the recently identified cytokine interleukin (IL)-13 on collagen-induced arthritis (CIA) was explored and compared to those IL-4 using systemic administration these cytokines via two injections xenogeneic vector cells transfected with a plasmid construct. CIA induced in DBA/I mice by immunization native bovine type II collagen (CII). Chinese hamster ovary (CHO) fibroblasts mouse IL-13 or genes were inoculated subcutaneously days 10 25 post-priming CII...
Abstract Over the past decade, central role of T cells in process collagen‐induced arthritis (CIA) has been extensively documented. The inflammatory features CIA and its successful modulation after treatment vivo with Th2 lymphokines, known to down‐regulate proinflammatory cytokines, classify as a Th1‐mediated disease. However, no direct evidence for presence different helper subsets obtained. To identify collagen‐specific CD4 + cell subset(s) developing during course CIA, lymph nodes from...
Treatment of human synovial cells with interleukin‐1 (IL‐1) results in a large increase the production prostaglandin E, (PGE 2 ), function which activation phospholipase A (PLA ) is key step. In order to identify enzymes that are linked IL‐1‐mediated arachidonate availability and subsequent PGE production, we have investigated changes gene expression 85‐kDa cytosolic PLA (cPLA 14‐kDa secretory (sPLA two forms cyclooxygenase synoviocytes after stimulation recombinant IL‐1β. Northern‐blot...
Abstract Immunization with native type II collagen (CII) of susceptible strains mice (H‐2 q ) induces a rheumatoid arthritis‐like disease. Collagen‐induced arthritis (CIA) is an experimental model for T cell‐mediated autoimmune To investigate the cell receptor (TcR) repertoire involved in pathogenesis CIA, CII‐primed DBA/1 were treated various TcR V bT ‐specific monoclonal antibodies (mAb) using protocol resulting long‐term elimination target cells. In vivo treatment anti‐CD4 mAb led to...
Immunisation with heterologous type II collagen (CII) induces arthritis in mice of the DBA/1 strain, which is genetically susceptible to this disease. To develop an experimental model autoimmunity more adequate for study human rheumatoid (RA), were injected 100 micrograms native CII that had been purified from mouse xiphoid cartilage. About six weeks later animals developed a chronic progressive polyarthritis involving four paws but mainly confined interphalangeal and metatarsophalangeal...
Thymic factor, cyclic AMP, and products increasing its cellular level, such as Prostaglandin E1, induce the appearance of theta-antigen on T-cell precursors whether assessed by a rossette-inhibition assay or cytotoxic after cell fractionation BSA discontinuous gradiet. Synergism has been demonstrated between AMPT TF for that effect. Conversely, decrease theta expression obtained altering AMP level in theta-positive cells either it dibutyryl cAMP treatment decreasing indomethacin treatment....
Spectrins, components of the membrane skeleton, are implicated in various cellular functions.Understanding diversity these functions requires better characterization interacting domains spectrins, such as SH3 domain.Yeast two-hybrid screening a kidney cDNA library revealed that domain ␣II-spectrin binds specifically isoform A low-molecular-weight phosphotyrosine phosphatase (LMW-PTP).The does not interact with LMW-PTP B or C nor ␣I-spectrin domain.The interaction spectrin led us to look for...
Abstract Native type II collagen, the major cartilage is immunogenic and arthritogenic in rodents. To investigate whether minor collagens are arthritogenic, we immunized DBA/1 mice with pepsin‐soluble fractions of IX or XI collagen emulsified Freund's complete adjuvant. Both were after only 1 injection. However, incidence polyarthritis was lower severity lesser than that induced by bovine even when a booster injection administered. All developed humoral response to immunizing antigen,...
Abstract We previously compared by microarray analysis gene expression in rheumatoid arthritis (RA) and osteoarthritis (OA) tissues. Among the set of genes identified as a molecular signature RA, clusterin (clu) was one most differentially expressed. In present study we sought to assess role CLU (mRNA protein) affected joints cultured fibroblast-like synoviocytes (FLS) determine its functional role. Quantitative RT-PCR, Northern blot, situ hybridization, immunohistochemistry, Western blot...
SUMMARY TNF-α is one of the major proinflammatory cytokines involved in pathogenesis chronic inflammatory joint disease, human rheumatoid arthritis as well murine models this disease. It was previously described that a highly destructive spontaneous develops mice expressing transgene modified with 3′ untranslated region β-globin. The present study investigates mouse model effects anti-inflammatory IL-4, IL-10 and IL-13 administered vivo on cytokine expression. Groups TNF-α-transgenic were...
The value of estimated glomerular filtration rate (eGFR) in living kidney donors screening is unclear. A recently published web-based application derived from large cohorts, but not donors, calculates the probability a measured GFR (mGFR) lower than determined threshold. Our objectives were to validate clinical utility this tool cohort and test two other strategies based on chronic disease epidemiology collaboration (CKD-EPI) MDRD-eGFR. was using 51Cr- ethylene-diamine tetraacetic acid...
Most of hereditary elliptocytosis (HE) cases are related to a spectrin dimer (SpD) self-association defect. The severity haemolysis is correlated with the extent SpD defect, which itself depends on location mutation regarding tetramerization site. This site presumed involve first C helix alpha chain and last two helices, A B, beta reconstitute triple helical structure (A, B C), as observed along spectrin. Using recombinant peptides, we demonstrated that helices alone not sufficient establish...