A5050122044- SARS-CoV-2 and COVID-19 Research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Plant Virus Research Studies
- CRISPR and Genetic Engineering
- Bacteriophages and microbial interactions
- COVID-19 Clinical Research Studies
- Acute Ischemic Stroke Management
- Viral Infections and Vectors
- EFL/ESL Teaching and Learning
- Lipid metabolism and disorders
- Virus-based gene therapy research
- Vaccine Coverage and Hesitancy
- Ophthalmology and Visual Impairment Studies
- COVID-19 diagnosis using AI
- Respiratory viral infections research
- Geography and Education Methods
- Viral Infections and Immunology Research
- Humor Studies and Applications
- Misinformation and Its Impacts
- Sentiment Analysis and Opinion Mining
- Dementia and Cognitive Impairment Research
- Cardiac Structural Anomalies and Repair
Helix (United States)
2021-2025
BC Centre for Disease Control
2021-2024
Aventura Hospital and Medical Center
2024
Public Health Laboratory
2022
Provincial Health Services Authority
2021
University of Guelph
2020
University of South Florida
2019
Hôpital Saint-Jacques
2005
Summary As of January 2021, the highly transmissible B.1.1.7 variant SARS-CoV-2, which was first identified in United Kingdom (U.K.), has gained a strong foothold across world. Because sudden and rapid rise B.1.1.7, we investigated prevalence growth dynamics this States (U.S.), tracking it back to its early emergence onward local transmission. We found that RT-qPCR testing anomaly S gene target failure (SGTF), observed U.K., reliable proxy for detection. sequenced 212 SARS-CoV-2 genomes...
This study reports on the displacement of Alpha (B.1.1.7) by Delta (B.1.617.2 and its substrains AY.1, AY.2, AY.3) in United States. By analyzing RT-qPCR testing results viral sequencing samples collected across States, we show that percentage SARS-CoV-2 positive cases caused dropped from 67% May 2021 to less than 3.0% just 10 weeks. We also variant has outcompeted Iota (B.1.526) interest Gamma (P.1) concern. An analysis mean quantification cycles (Cq) values tests over time reveal...
ABSTRACT The ongoing COVID-19 pandemic necessitates cost-effective, high-throughput, and timely whole-genome sequencing (WGS) of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) viruses for outbreak investigations, identifying variants concern (VoC), characterizing vaccine breakthrough infections, public health surveillance. In addition, the enormous demand WGS on supply chains resulting shortages laboratory supplies necessitated use low-reagent low-consumable methods. Here, we...
Abstract Between November 2021 and February 2022, SARS-CoV-2 Delta Omicron variants co-circulated in the United States, allowing for co-infections possible recombination events. We sequenced 29,719 positive samples during this period analyzed presence fraction of reads supporting mutations specific to either or variant. identified 18 co-infections, one which displayed evidence a low Delta-Omicron recombinant viral population. also two independent cases infection by virus, where 100% RNA came...
As part of a multi-state viral genomic surveillance program, we conducted case-only analysis to evaluate the effectiveness XBB.1.5-adapated mRNA vaccines in preventing severe illness among individuals with medically attended SARS-CoV-2 infection. We compared prior receipt an XBB.1.5-adapted vaccine between SARS-CoV-2-infected adults inpatient or emergency department (ED) visits (as proxy for illness) vs those outpatient mild illness). Among 6,551 patients September 2023 and January 2024,...
The rapid emergence of SARS-CoV-2 variants raised public health questions concerning the capability diagnostic tests to detect new strains, efficacy vaccines, and how map geographical distribution understand transmission patterns loads on healthcare resources. Next-generation sequencing (NGS) is primary method for detecting tracing variants, but it expensive, can take weeks before sequence data are available in repositories. This article describes a customizable reverse transcription PCR...
ABSTRACT Within a multi-state viral genomic surveillance program, we conducted case-control analysis comparing prior receipt of XBB.1.5-adapted mRNA vaccination between SARS-CoV-2-infected adults with inpatient/ED visits (proxy for severe illness) vs outpatient visits. Among 6,551 patients from September 2023-January 2024, 6.1% 12.0% had received XBB.1.5 (aOR=0.41; 95%CI:0.32-0.53). This protective association was weaker among JN.1 (aOR=0.62; 95%CI:0.40-0.96) XBB-lineage (aOR=0.28;...
Mutations in emerging severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) lineages can interfere with laboratory methods used to generate viral genome sequences for public health surveillance. We identified 20 mutations that are widespread variant of concern and affect widely sequencing protocols by the ARTIC network Freed et al. Three these disrupted P.1 lineage specimens during a recent outbreak British Columbia, Canada. provide validation protocol modifications restored...
This demo showcases an interactive virtual reality experience for language learning that allows users to enter a world explore and interact with their surroundings while Spanish. Through immersive game-play on the Oculus Rift, Spanish translations of everyday household items in search-and-find format, scoring points when they can correctly identify select objects. Users are able put what learn into practice real time. Study participants who tried said found this method be more enjoyable than...
Lyme disease, caused by Borrelia burgdorferi sensu lato (s.l.) complex, is the most common vector-borne disease in North America. This has a much lower incidence western compared with eastern Passive tick surveillance data submitted over 17 years from 2002 to 2018 were analyzed determine occurrence of species and prevalence spp. ticks British Columbia (BC), Canada. The BC Centre for Disease Control Public Health Laboratory received submissions physicians, veterinarians, residents. Ticks...
Abstract Within a multistate viral genomic surveillance program, we evaluated whether proportions of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections attributed to the JN.1 variant and XBB-lineage variants (including HV.1 EG.5) differed between inpatient outpatient care settings during periods cocirculation. Both were less likely than EG.5 account for among inpatients versus outpatients (adjusted odds ratio [aOR], 0.60 [95% confidence interval (CI), .43–.84; P = .003]...
ABSTRACT The ongoing COVID-19 pandemic necessitates cost-effective, high-throughput, and timely genomic sequencing of SARS-CoV-2 viruses for outbreak investigations, identifying variants concern (VoC), characterizing vaccine breakthrough infections, public health surveillance. Additionally, the enormous demand on supply chains resulting shortages laboratory supplies necessitate use low-reagent low-consumable methods. Here, we report an optimized library preparation method where same protocol...
Within a multistate clinical cohort, SARS-CoV-2 antiviral prescribing patterns were evaluated from April 2022-June 2023 among nonhospitalized patients with risk factors for severe COVID-19. Among 3247 adults, only 31.9% prescribed an agent (87.6% nirmatrelvir/ritonavir, 11.9% molnupiravir, 0.5% remdesivir), highlighting the need to identify and address treatment barriers.
Animal study results have suggested a role in sepsis for human interleukin DA1.a cells/leukemia inhibitory factor (HILDA/LIF). HILDA/LIF and interleukin-6 (IL-6) levels were prospectively studied by serial ELISAs 34 septic patients. was detected 11 of patients at plasma 100-37,000 pg/mL. Peak correlated with increased temperature creatinine IL-6 decreased arterial CO2 (P < .05). Multivariate analysis showed that shock accounted 75% peak variations (R2 = .753). Fatal outcome most often...
ABSTRACT Within a multi-state viral genomic surveillance program, proportions of SARS-CoV-2 infections attributed to the JN.1 and HV.1 variants, compared EG.5, were each lower among inpatients versus outpatients (aOR=0.33 [95% CI: 0.20-0.55] aOR=0.62 0.44-0.86], respectively). variants may be associated with risk severe illness.
The COVID-19 pandemic has highlighted the need for generic reagents and flexible systems in diagnostic testing. Magnetic bead-based nucleic acid extraction protocols using 96-well plates on open liquid handlers are readily amenable to meet this need. Here, one such approach is rigorously optimized minimize cross-well contamination while maintaining sensitivity.
Abstract The rapid emergence of new SARS-CoV-2 variants raises a number public health questions including the capability diagnostic tests to detect strains, efficacy vaccines, and how map geographical distribution better understand patterns transmission possible load on healthcare resources. Next-Generation Sequencing (NGS) is primary method for detecting tracing variants, but it expensive, can take weeks before sequence data available in repositories. Here, we describe Polymerase Chain...
Between November 2021 and February 2022, SARS-CoV-2 Delta Omicron variants co-circulated in the United States, allowing for co-infections possible recombination events. We sequenced 29,719 positive samples during this period analyzed presence fraction of reads supporting mutations specific to either or variant. identified 18 co-infections, one which displayed evidence a low Delta-Omicron recombinant viral population. also two independent cases infection by virus, where 100% RNA came from...
The sequencing of 74,348 SARS-CoV-2 positive samples collected across the United States showed that Delta variant was responsible for majority infections by July 1, 2021, and accounted >99.9% tests in September 2021. displaced Alpha, which dominant at time. also directly outcompeted Gamma concern as well Iota Mu variants interest. An analysis mean quantification cycles (Cq) values revealed lead to a higher viral load on average compared Alpha infections, but this increase only ~1.7x...
With the advent of COVID-19 pandemic, people have flocked to social media in order stage their thoughts surrounding these unusual circumstances. This paper aims uncover public sentiment regarding novel coronavirus pandemic on microblogging platform Twitter. is done through a proposed algorithm that builds off existing aspect-based analysis approaches and opts for Naïve-Bayes route classify Tweets been atomized into n-grams. research concludes overall outbreak over July 2020 combination...
Viral myocarditis is a serious complication of viral infections that known to impact young adults and can result in significant cardiac issues like earlier onset heart failure, arrhythmia, or structural disease if not detected treated promptly. This case report focusing on 25-year-old woman diagnosed with highlighting the diagnostic difficulties it present due its diverse symptoms. Following thorough workup appropriate treatment, including diuretics, antibiotics, guideline-directed medical...