A5069834042- Cardiac Ischemia and Reperfusion
- Retinal Development and Disorders
- Nitric Oxide and Endothelin Effects
- Renal and Vascular Pathologies
- Traumatic Brain Injury and Neurovascular Disturbances
- Antioxidant Activity and Oxidative Stress
- Glaucoma and retinal disorders
- Intraocular Surgery and Lenses
- Nicotinic Acetylcholine Receptors Study
- Animal testing and alternatives
- Cholinesterase and Neurodegenerative Diseases
- Adenosine and Purinergic Signaling
- Retinal Diseases and Treatments
- Renin-Angiotensin System Studies
- RNA regulation and disease
- Hedgehog Signaling Pathway Studies
- Genetic and rare skin diseases.
- Cardiac and Coronary Surgery Techniques
- bioluminescence and chemiluminescence research
- Renal function and acid-base balance
- Cancer and Skin Lesions
- Receptor Mechanisms and Signaling
- Tryptophan and brain disorders
- Chemotherapy-induced organ toxicity mitigation
- Ocular Disorders and Treatments
Hamamatsu University School of Medicine
2006-2022
Hamamatsu University
1996-2017
Photonics (United States)
2015
Tokyo University of Science
2012
Park Terrace Care Center
2007
University of Shizuoka
1998
Food & Nutrition
1998
Tulane University
1992-1995
We recently developed a matrix-assisted laser desorption/ionization quadrupole ion trap time-of-flight (MALDI-QIT-TOF)-based imaging mass spectrometry (IMS) system. This system enables us to perform structural analyses using tandem (MS/MS), as well visualize phospholipids and peptides in frozen sections. In the retina, phototransduction is regulated by light-sensitive interaction between visual pigment-coupled receptor proteins, such rhodopsin, G transducin. There are some reports that...
The in vitro blood-perfused juxtamedullary nephron technique was utilized to determine the contribution of endothelium-derived relaxing factor (EDRF) resting renal arteriolar caliber and evaluate interaction between EDRF angiotensin II (ANG II) microvascular control. Video microscopy employed visualize rat afferent efferent arterioles measure their responses blockade nitric oxide (NO), which has been shown account for much biological action EDRF. NO synthesis inhibitor, N...
Experiments were performed to determine the influence of endogenous nitric oxide (NO) on basal arteriolar diameter in kidneys from diabetic rats and evaluate role superoxide anions as modulators NO activity under these conditions. Male Sprague-Dawley injected with streptozotocin (STZ, 65 mg/kg i.v.) received insulin via ip osmotic minipumps (3 U/kg per day). Sham vehicle treatments. Videomicroscopy was used, conjunction vitro blood-perfused juxtamedullary nephron technique, visualize renal...
Based on evidence that extracellular ATP can influence vascular smooth muscle function in other organ systems, experiments were conducted to characterize the responsiveness of rat juxtamedullary microvascular segments ATP. Experiments performed using vitro blood-perfused nephron preparation combined with video microscopy. Pentobarbital-anesthetized rats pretreated enalaprilat (2 mg iv) for 30 min before right kidney was isolated and prepared study. Renal perfusion pressure set at 110 mmHg...
Experiments were performed to test the hypothesis that diabetes mellitus is associated with impaired afferent arteriolar responsiveness opening of voltage-gated calcium channels. Diabetes was induced by injection streptozocin (65 mg/kg, i.v.) and insulin administered via an osmotic minipump achieve moderate hyperglycemia. Sham rats received vehicle treatments. 2 wk later, in vitro blood-perfused juxtamedullary nephron technique used allow videomicroscopic measurement contractile responses...
The first synthesis of decahydrofluorene core 4 pyrrocidines was accomplished. cis,trans-fused tricyclic ring system stereoselectively constructed via Diels–Alder reaction using two Danishefsky dienes.
Previous studies have suggested a paracrine role for extracellular ATP in the regulation of afferent arteriolar tone. The current study was conducted to determine dependence this response on calcium entry mechanisms. Experiments were performed vitro using blood-perfused juxtamedullary nephron technique combined with video microscopy. alpha, beta-methylene determined before and after treatment channel blockers, diltiazem or felodipine. beta-Methylene used obviate concerns over responses being...
This study was designed to identify and localize defects in renal microvascular function during the hyperfiltration stage of diabetes mellitus. Male Sprague-Dawley rats were injected intravenously with 65 mg/kg streptozotocin (IDDM rats) or vehicle (sham rats). IDDM received insulin (3 U.kg-1.day-1) via an osmotic minipump; sham diluent. During ensuing 2-wk period, blood glucose levels averaged 89 +/- 2 mg/dl 33 290 13 37 rats. At end this inulin clearance elevated eight (1.43 0.17...
Studies were performed to examine whether atrial natriuretic peptide (ANP) has a direct action on glomerular afferent arterioles, and if so, the is mediated by guanosine 5'-cyclic monophosphate (cGMP). A single superficial arteriole was dissected from canine kidney perfused with perfusion technique described Osgood et al. [Am. J. Physiol. 244 (Renal Fluid Electrolyte 13): F349-F354, 1983]. Norepinephrine (NE, 1 x 10(-6) M) significantly increased arteriolar resistance, calculated rate...
We investigated the iron release from ferritin by irradiation a white fluorescent light in absence or presence of ADP. Irradiation solution at 17,000 lx ADP slightly induces but only acidic pH conditions (pH 5.0 6.0). markedly enhances under same conditions. In irradiation, was low even The induction also affected various factors such as dose and acidity, not temperature (4-47 degrees C), oxygen concentration, free radical generations during irradiation. ceased to increase turning off found...
Purpose: To identify mutations in the RDH5 gene a family with mother having fundus albipunctatus (FA) and 3 children retinitis pigmentosa (RP). Methods: Ophthalmological examinations were performed to diagnose FA RP. Mutational analysis of was performed. Results/Conclusions: The diagnosed FA, proband's mother, brother, sister had novel mutation c.689_690CT > GG RDH5. proband previously reported c.928delCinsGAAG. Consequently, mother's caused by compound heterozygous mutations. Further...
Although the gene encoding optineurin (OPTN) is a causative for glaucoma and amyotrophic lateral sclerosis, it ubiquitously expressed in all body tissues, including retina. To study function of OPTN retinal ganglion cells as well whole retina, we previously isolated OPTN-interacting proteins identified bZIP transcription factor neural retina leucine zipper (NRL), which retinitis pigmentosa. Herein, investigated binding between NRL HeLaS3 cells. Co-expression HA-tagged FLAG-tagged followed by...
We studied the synergistic effect of visible light and ferritin on lipid peroxidation a fraction porcine photoreceptor outer segment (POS). Reaction mixtures containing POS horse spleen were irradiated under white fluorescent mainly at 17,000 lx or incubated dark conditions 37 degrees C. The was evaluated by both thiobarbituric acid method ferrous oxidation/xylenol orange method. irradiation-induced affected some experimental factors such as irradiation dose acidity material. When stopped,...
We analyzed two siblings in a Japanese family with delayed onset cone-rod dystrophy (CRD) using whole-exome sequencing. A novel frameshift c.1106dup (p.H370Afs*17) variant and known missense c.2027 T > (p.I676N) CDHR1 were identified. Both patients shared the same variants, although they displayed significant difference disease severity. meta-analysis of relationship between severity type was performed reported cases literature did not reveal definitive correlation.
Studies were performed on previously nephrectomized dogs to examine roles of hormonal factors in plasma potassium alterations acute alkalosis. Respiratory and metabolic alkalosis induced by hyperventilation intravenous NaHCO3 or tris(hydroxymethyl)aminomethane (Tris) infusion, respectively. NaHCO3-induced provoked decreases from the control value 5.12 +/- 0.68 (SE) 4.21 0.55 meq/l (P less than 0.01) 4.65 0.26 3.91 0.16 within 180 min, In contrast, Tris-induced elicited an increase 4.56 0.30...
Abstract Photoisomerization of lipids has been well studied. As for the eyes, photoisomerization from 11- cis isomer to all- trans -retinal is well-known as first step visual transduction in photoreceptors. In addition that, there would be other ocular that undergo photoisomerization, which may involved health and function. To explore any photoisomerizable nonirradiated sunlight-irradiated eyeball extracts were subjected liquid chromatography-mass spectrometry analysis, followed by...