A5046758635- Immune Cell Function and Interaction
- Cannabis and Cannabinoid Research
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- Cancer Immunotherapy and Biomarkers
- CAR-T cell therapy research
- Immunotherapy and Immune Responses
- Immune cells in cancer
- Cancer-related molecular mechanisms research
- Dietary Effects on Health
- Microbial Inactivation Methods
- Pancreatic function and diabetes
- Transgenic Plants and Applications
- Microfluidic and Bio-sensing Technologies
- Reproductive System and Pregnancy
- Immune Response and Inflammation
- Head and Neck Cancer Studies
- Asthma and respiratory diseases
- MicroRNA in disease regulation
- Inflammation biomarkers and pathways
- Alcohol Consumption and Health Effects
- Nanoparticle-Based Drug Delivery
- Lung Cancer Research Studies
- Acute Myeloid Leukemia Research
- IL-33, ST2, and ILC Pathways
Dana-Farber Cancer Institute
2016-2022
Harvard University
2016-2022
University of South Carolina
2013-2016
Significance Depletion or inhibition of regulatory T cells (Tregs) has been associated with increased effector T-cell activation that may enhance antitumor responses. A potentially more effective strategy depends on induction lineage instability allows conversion intratumoral but not systemic Tregs into (Teffs). We show targeted deletion the Helios transcription factor within CD4 promotes and cell in tumors immunity. Ab-dependent ligation Treg surface receptors diminishes expression can also...
MDSCs are potent immunosuppressive cells that induced during inflammatory responses, as well by cancers, to evade the anti-tumor immunity. We recently demonstrated marijuana cannabinoids inducers of MDSCs. In current study, we investigated epigenetic mechanisms through which THC, an exogenous cannabinoid, induces and compared such with naïve found in BM BL6 (WT) mice. Administration THC into WT mice caused increased methylation at promoter region DNMT3a DNMT3b THC-induced MDSCs, correlated...
Follicular regulatory T (TFR) cells are a specialized suppressive subset that controls the germinal center (GC) response and maintains humoral self-tolerance. The mechanisms maintain TFR lineage identity activity remain largely unknown. Here, we show expression of Blimp1 by FoxP3+ is essential for stability, entry into GC, activity. Deletion in reduced FoxP3 CTLA-4 increased pro-inflammatory cytokines spontaneous production autoantibodies, including elevated IgE. Maintenance stability...
Abstract Immune cells have been shown to express cannabinoid receptors and produce endogenous ligands. Moreover, activation of on immune has trigger potent immunosuppression. Despite such studies, the role cannabinoids in transplantation, specifically prevent allograft rejection, not, our knowledge, investigated previously. In current study, we tested effect THC suppression HvGD as well rejection skin allografts. To this end, studied by injecting H-2k splenocytes into H-2b mice analyzing...
Endocannabinoids are endogenous ligands for the cannabinoid (CB) receptors which include anandamide (AEA) and 2-arachidonyl glycerol (2-AG). 2-AG has been linked to inflammation due its elevated expression in animal models of autoimmunity hypersensitivity. However, administration exogenous shown suppress making precise role unclear. In current study, we investigated following immunization C57BL/6 (BL6) mice with methylated BSA (mBSA) antigen, triggers both delayed-type hypersensitivity (DTH)...
Significance Regulatory T cells rely on active processes to maintain a suppressive phenotype inside tumor, leading increased tumor burden and worse cancer outcomes. Here, we report pathway interfere with regulatory cell (Treg) stability by disrupting the expression of interleukin 23 receptor. This approach increases Treg responsiveness 12, production gamma-interferon more efficient antitumor immune responses. The combined engagement independent pathways destabilize through receptor...
Abstract Non-viral approaches to transfection have emerged a viable option for gene transfer. Electro-mechanical involving use of electric fields coupled with high fluid flow rates is scalable strategy cell therapy development and manufacturing. Unlike purely field-based or mechanical-based delivery methods, the combined effects result in genetic material at efficiencies low toxicity. This study focuses on reporter mRNA show electro-mechanical can be used successfully human T cells. Rapid...
Programmed cell death-1(PD-1)/Programmed death ligand-1 (PD-L1) immune check blockade is one remarkable advance in cancer immunotherapy treatment. Clinical trials studying the efficacy of PD-1 alone or combination with other therapy different types have been evaluated are under observation. Nasopharyngeal carcinoma, also called Cantonese cancer, occurs rarely western countries but common tumors head and neck South China. NPC type tumors, it quite distinct among tumors. Here, we would like to...
Abstract The mechanism of immune regulation by cannabinoids remains unclear. Δ-9-tetrahydrocannabinol (THC) is the most widely studied cannabinoid isolated from Cannabis sativa plant. Our laboratory has previously shown that, in mice, THC induces myeloid derived suppressor cells (MDSCs), a heterogeneous population immunoregulatory cells. In this study, we focused on role MDSC expansion and generation. Intraperitoneal injection induced local MDSCs which were able to suppress T-cell...
Abstract Interleukin 23 receptor (IL23R) is a signaling protein normally implicated in gut T cell development, but which has also been correlated with poor cancer prognoses. Previous studies have found that interfering this pathway results improved outcomes mice. These blocking IL23R was more effective than neutralizing IL23, suggesting an IL23R-expressing responsible for the tumor-protective effects observed targeting IL23R. Here, we show Treg-specific of provides potent tumor immunity...