The present work demonstrates the use of a dielectrophoretic lab-on-a-chip device in effectively separating different cancer cells epithelial origin for application circulating tumor cell (CTC) identification. This study uses dielectrophoresis (DEP) to distinguish and separate MCF-7 human breast from HCT-116 colorectal cells. DEP responses each type were measured against AC electrical frequency changes solutions varying conductivities. Increasing conductivity suspension directly correlated...

We examined the importance of histone methylation by polycomb group proteins in mouse circadian clock mechanism. Endogenous EZH2, a enzyme that methylates lysine 27 on H3, co-immunoprecipitates with CLOCK and BMAL1 throughout cycle liver nuclear extracts. Chromatin immunoprecipitation revealed EZH2 binding di- trimethylation H3K27 both Period 1 2 promoters. A role cryptochrome-mediated transcriptional repression clockwork was supported overexpression RNA interference studies. Serum-induced...

Colorectal cancer risk is increased in shift workers with presumed circadian disruption. Intestinal epithelial cell proliferation gated throughout each day by the clock. Period 2 (Per2) a key clock gene. Per2 mutant (Per2(m/m)) mice show an increase lymphomas and deregulated expression of cyclin D c-Myc genes that are to control. We asked whether gene inactivation would accelerate intestinal colonic tumorigenesis. The effects PER2 on beta-catenin were studied colon lines its down-regulation...

MicroRNAs (miRNAs) decrease the expression of specific target oncogenes or tumor suppressor genes and thereby play crucial roles in tumorigenesis growth. To date, potential miRNAs regulating osteosarcoma growth progression are not fully identified yet. In this study, miRNA microarray assay hierarchical clustering analysis were performed human samples. comparison with normal skeletal muscle, 43 significantly differentially expressed osteosarcomas (fold change ≥2 p≤0.05). Among these miRNAs,...

Abstract Post-traumatic stress disorder patients experience chronic systemic inflammation. However, the molecular pathways involved and mechanisms regulating expression of genes in inflammatory PTSD are reported inadequately. Through RNA sequencing miRNA microarray, we identified 326 190 miRNAs that were significantly different their levels PBMCs patients. Expression pairing differentially expressed indicated an inverse relationship expression. Functional analysis involvement canonical...

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) has diverse biological functions including its nuclear translocation in response to oxidative stress. We show that GAPDH physically associates with APE1, an essential enzyme involved the repair of abasic sites damaged DNA, as well redox regulation several transcription factors. This interaction allows convert oxidized species APE1 reduced form, thereby reactivating endonuclease activity cleave sites. The variants C152G and C156G retain ability...

Cell cycle progression is tightly regulated. The expressions of cell regulators, the products which either promote or inhibit proliferation, oscillate during each cycle. Cellular proliferation and expression regulators are also controlled by circadian clock. Disruption clock may thereby lead to deregulated proliferation. Mammalian Per2 a core gene, product suppresses cancer tumor growth in vivo vitro. Because Per1, another key mutated human breast cancers, because its functions similar...

Separation of colorectal cancer cells from other biological materials is important for stool-based diagnosis cancer. In this paper, we use conventional dielectrophoresis in a microfluidic chip to manipulate and isolate HCT116 cells. It noticed that at particular alternating current frequency band, the are clearly deflected side channel main after electric activation an electrode pair. This motion caused by negative can be used simply rapidly separate manuscript, report design, flow...

Timeless (Tim), a core circadian clock gene in Drosophila, is retained mammals but has no apparent mammalian function. Mammalian TIM essential for ATR-dependent Chk1 activation and S-phase arrest. We report that likewise ATM-dependent Chk2-mediated signaling of doxorubicin-induced DNA double strand breaks. depletion attenuates G(2)/M cell cycle arrest sensitizes cancer cells to cytotoxicity. is, thereby, potential novel anticancer drug target whose inhibition may enhance the therapeutic...

Abstract Aging is associated with increased monocyte production and altered function. Classical monocytes are heterogenous a shift in their subset composition may underlie some of apparent functional changes during aging. We have previously shown that mouse granulocyte‐monocyte progenitors (GMPs) produce “neutrophil‐like” (NeuMo), whereas monocyte‐dendritic cell (MDPs) monocyte‐derived dendritic (moDC)‐producing (DCMo). Here, we demonstrate classical from the bone marrow old male female mice...

Period genes ( Per2, Per1) are essential circadian clock genes. They also function as negative growth regulators. Per2 mutant mice show de novo and radiation-induced epithelial hyperplasia, tumors, an abnormal DNA damage response. Human tumors gene mutations or decreased expression. Other murine not associated with a tumor prone phenotype. Shift work nocturnal light exposure disruption increased cancer risk. The mechanisms responsible for the connection between well defined. We propose that...

Polo-like kinase 1 (Plk1) overexpression is associated with tumorigenesis by an unknown mechanism. Likewise, Plk1 was suggested to act as a negative regulator of tumor suppressor p53, but the mechanism remains be determined. Herein, we have identified topoisomerase I-binding protein (Topors), p53-binding protein, target. We show that phosphorylates Topors on Ser(718) in vivo. Significantly, expression Plk1-unphosphorylatable mutant (S718A) leads dramatic accumulation p53 through inhibition...

Maintenance of cancer stem cells (CSCs) is regulated by the tumor microenvironment. Synthetic hydrogels provide flexibility to design three-dimensional (3D) matrices isolate and study individual factors in The objective this work was investigate effect matrix modulus on tumorsphere formation breast maintenance CSCs an inert microenvironment without interference other factors. In that regard, 4T1 mouse were encapsulated polyethylene glycol diacrylate expression CSC markers investigated. gel...

MDSCs are potent immunosuppressive cells that induced during inflammatory responses, as well by cancers, to evade the anti-tumor immunity. We recently demonstrated marijuana cannabinoids inducers of MDSCs. In current study, we investigated epigenetic mechanisms through which THC, an exogenous cannabinoid, induces and compared such with naïve found in BM BL6 (WT) mice. Administration THC into WT mice caused increased methylation at promoter region DNMT3a DNMT3b THC-induced MDSCs, correlated...

Introduction As cancer cells are affected by many factors in their microenvironment, a major challenge is to isolate the effect of specific factor on stem (CSCs) while keeping other unchanged. We have developed synthetic inert 3D polyethylene glycol diacrylate (PEGDA) gel culture system as unique tool study microenvironmental CSCs response. reported that formed PEGDA encapsulation breast maintain stemness within certain range stiffness. The objective was investigate CD44 binding peptide...

Abstract Long noncoding RNAs (lncRNAs) have been demonstrated to play important regulatory roles in gene expression, from histone modification protein stability. However, the functions of most identified lncRNAs are not known. In this study, we investigated role an lncRNA called AW112010. The expression AW112010 was significantly increased CD4+ T cells C57BL/6J mice activated vivo with myelin oligodendrocyte glycoprotein, Staphylococcal enterotoxin B, or vitro anti-CD3 anti-CD28 mAbs,...

The 8-oxo-7,8-dihydrodeoxyguanosine (8oxoG), a major mutagenic DNA lesion, results either from direct oxidation of guanines or misincorporation 8oxodGTP by polymerases. At present, little is known about the mechanisms preventing action in Saccharomyces cerevisiae. Herein, we report for first time identification an alternative repair pathway 8oxoG residues initiated S. cerevisiae AP endonuclease Apn1, which endowed with robust progressive 3′→5′ exonuclease activity towards duplex DNA. We show...