A5100740693- Microtubule and mitosis dynamics
- Cancer-related Molecular Pathways
- DNA Repair Mechanisms
- Supramolecular Chemistry and Complexes
- Glaucoma and retinal disorders
- Retinal Diseases and Treatments
- Cancer-related gene regulation
- Synthesis and Characterization of Heterocyclic Compounds
- Ubiquitin and proteasome pathways
- Protein Degradation and Inhibitors
- Corneal surgery and disorders
- Ophthalmology and Visual Impairment Studies
- Genomics and Chromatin Dynamics
- RNA modifications and cancer
- Adipose Tissue and Metabolism
- DNA and Nucleic Acid Chemistry
- Sirtuins and Resveratrol in Medicine
- Mitochondrial Function and Pathology
- Cancer therapeutics and mechanisms
- Cellular transport and secretion
- Prostate Cancer Treatment and Research
- Alzheimer's disease research and treatments
- Ophthalmology and Eye Disorders
- Radiopharmaceutical Chemistry and Applications
- Tryptophan and brain disorders
Center for Excellence in Molecular Cell Science
2021-2025
University of Chinese Academy of Sciences
2021-2025
Shanxi Academy of Medical Sciences
2023-2025
Shanxi Medical University
2025
Huazhong University of Science and Technology
2025
Tongji Hospital
2025
Purdue University West Lafayette
2011-2024
University of Kentucky
2023-2024
Guangzhou University of Chinese Medicine
2018-2024
Key Laboratory of Guangdong Province
2024
Elevated expression of mammalian polo-like kinase (Plk)1 occurs in many different types cancers, and Plk1 has been proposed as a novel diagnostic marker for several tumors. We used the recently developed vector-based small interfering RNA technique to specifically deplete cancer cells. found that depletion dramatically inhibited cell proliferation, decreased viability, resulted cell-cycle arrest with 4 N DNA content. The formation dumbbell-like chromatin structure suggests inability these...
We previously reported the phenotype of depletion polo-like kinase 1 (Plk1) using RNA interference (RNAi) and showed that p53 is stabilized in Plk1-depleted cancer cells. In this study, we further analyzed Plk1 depletion-induced both cells primary The vector-based RNAi approach was used to evaluate role pathway apoptosis with different backgrounds. Although DNA damage cell death can occur independently p53, p53-deficient were much more sensitive than functional p53. Next, lentivirus-based...
Myopia is the most common ocular disorder worldwide, and high myopia in particular one of leading causes blindness. Genetic factors play a critical role development myopia, especially myopia. Recently, exome sequencing approach has been successfully used for disease gene identification Mendelian disorders. Here we show successful application to identify an autosomal dominant disorder, have identified potentially responsible monogenic form. We captured exomes two affected individuals from Han...
Microenvironmental oxygen (O2) regulates stem cell activity, and a hypoxic niche with low levels has been reported in multiple types. Satellite cells are muscle-resident that maintain the homeostasis mediate regeneration of skeletal muscles. We demonstrate here culture conditions favor quiescence satellite cell-derived primary myoblasts by upregulating Pax7, key regulator self-renewal, downregulating MyoD myogenin. During myoblast division, hypoxia promotes asymmetric self-renewal divisions...
The natural polyamine spermidine and spermine have been reported to ameliorate aging aging-induced dementia.However, the mechanism is still confused.An model, senescence accelerated mouse-8 (SAMP8), was used in this study.Novel object recognition open field test results showed that oral administration of spermidine, rapamycin increased discrimination index, modified number, inner squares distance times.Spermidine activity SOD, decreased level MDA brain.Spermidine phosphorylate AMPK regulate...
Abstract Epidemiological studies have found that diabetes and cognitive dysfunction are closely related. Quercetin has been certified with the effect on improving mellitus (DM) impairment. However, related mechanism of quercetin diabetic encephalopathy (DE) still ambiguous. In this study, we used db/db mice (diabetic model) to discover whether could improve DE through Sirtuin1/NLRP3 (NOD‐, LRR‐ pyrin domain‐containing 3) pathway. Behavioural results (Morris water maze new object recognition...
Brown adipose tissue (BAT) dissipates energy through Ucp1-mediated uncoupled respiration and its activation may represent a therapeutic strategy to combat obesity. Here we show that Lkb1 controls BAT expansion UCP1 expression in mice. We generate adipocyte-specific knockout mice that, compared with wild-type littermates, these exhibit elevated subcutaneous white tissue, have increased mass higher expenditure. Consequently, KO improved glucose tolerance insulin sensitivity, are more resistant...
The events of the cell cycle, stages at which proliferates and divides, are facilitated controlled by multiple signaling pathways. Among many regulatory enzymes that contribute to these processes is polo-like kinase (Plk). Plks have been reported mediate mitotic processes, including bipolar spindle formation, activation Cdc25C, actin ring centrosome maturation, anaphase-promoting complex. To investigate its functions in mammalian cells further, we used recently developed small interfering...
Although the essential function of checkpoint kinase 1 (Chk1) in DNA damage response has been well established, role Chk1 normal cell cycle progression is unclear. By using RNAi to specifically deplete Chk1, we determined loss-of-function phenotypes HeLa cells. A vector-based approach showed that required for proliferation and survival, inasmuch as a dramatic cell-cycle arrest at G(2)/M phase massive apoptosis were observed Chk1-deficient Coupling siRNA with synchronization further revealed...