A5046495453- Antibiotics Pharmacokinetics and Efficacy
- Antibiotic Resistance in Bacteria
- Pneumonia and Respiratory Infections
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Muscle Physiology and Disorders
- Mycobacterium research and diagnosis
- Pharmaceutical and Antibiotic Environmental Impacts
- Acute Myocardial Infarction Research
- Clinical Reasoning and Diagnostic Skills
- Antibiotic Use and Resistance
- Medical Malpractice and Liability Issues
- Periodontal Regeneration and Treatments
- SARS-CoV-2 and COVID-19 Research
- Analytical chemistry methods development
- Analytical Chemistry and Chromatography
- Coronary Interventions and Diagnostics
- Vascular Malformations Diagnosis and Treatment
- Genital Health and Disease
- Economic and Social Development
- Respiratory and Cough-Related Research
- Bacterial biofilms and quorum sensing
- Patient-Provider Communication in Healthcare
- Chronic Disease Management Strategies
Universidad Complutense de Madrid
2008-2024
Johns Hopkins University
2023-2024
Clínica Alemana
2023
Universidad del Desarrollo
2023
Pontificia Universidad Católica de Chile
2016-2023
Duke University Hospital
2023
Duke Medical Center
2023
Universidad Nacional del Sur
2018-2023
Consejo Nacional de Investigaciones Científicas y Técnicas
2018-2023
University of Puerto Rico, Medical Sciences Campus
2023
ObjectivesTo explore serum and tissue pharmacodynamics of linezolid versus vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates with different MBC/MIC ratios.
Bactericidal activity depends on antibiotic-bacteria couples, resistance phenotype and theoretically protein binding. This work explores the influence of binding bactericidal two antibiotics, daptomycin versus vancomycin, that exhibit, respectively, different C(max) (56 25.5 mg/L), (91.7% 36.9%) thus theoretical free-drug fractions (4.7 16.1 mg/L).The effect presence physiological concentrations human albumin (4 g/dL) or serum (90%) was studied against Gram-positive isolates with troublesome...
In the context of validating a measure patient report specific to diagnostic accuracy in emergency department or urgent care, this study investigates patients' and care partners' perceptions diagnoses as accurate explores variations how they reason while assess accuracy.
An interview tool, Diagnostic Interview for Genetic Studies (DIGS 3.0), was translated into Spanish application in studies of psychiatric disorders Colombia. Two translations the original English version DIGS were prepared and back-translated English. A review committee verified linguistic cultural equivalence translations. The evaluator test-retest reliability assessed calculating Cohen's kappa samples 65 91 patients respectively. proved valid both appearance content. confidence interval...
Skeletal muscle regeneration and long term maintenance is directly link to the balance between self-renewal differentiation of resident adult stem cells known as satellite cells. In turn, cell fate influenced by a functional interaction transcription factor Pax7 members MyoD family regulatory factors. Thus, changes in Pax7-to-MyoD protein ratio may act molecular rheostat fine-tuning acquisition lineage identity while preventing precocious terminal differentiation. expressed quiescent...
Background Emergency and urgent care settings face challenges with routinely obtaining performance feedback related to diagnostic care. Patients their partners provide an important perspective on the process outcome of in these settings. We sought develop test psychometric properties Patient-Report IMprove Diagnostic Excellence Department (PRIME-ED), a measure patient-reported excellence Methods developed PRIME-ED based literature review, expert feedback, cognitive testing. To assess...
ObjectivesAttempts to interpret antibiotic pharmacodynamics using reported protein binding may underestimate true activity. To elucidate this issue we examined bacterial killing kinetics at cefditoren concentrations equal Cmax in the presence of 90% human serum or albumin physiological concentrations.
Background Specific antibodies are likely to be present before S. pneumoniae infection. We explored cefditoren (CDN) total and free values of serum concentrations exceeding the MIC (t>MIC) related efficacy in a mice sepsis model, effect specific gammaglobulins on in-vitro phagocytosis in-vivo efficacy. Methodology/Principal Findings used three pneumococcal isolates (serotype, CDN): Strain 1 (6B, µg/ml), 2 (19F, µg/ml) 3 (23F, 4 µg/ml). Hyperimmune (HS) was obtained from immunized with...
Mycobacterium avium subsp. paratuberculosis (MAP) is the causative agent of paratuberculosis, a contagious infectious disease that affects domestic and wild ruminants causing chronic inflammation intestine. MAP has proven to be very resistant both physical chemical processes, making it difficult control this pathogen. Based on recognized antimicrobial properties copper, objective study was evaluate effectiveness copper ions reduce numbers and/or viability in fluid matrix. Besides,...
Chronic kidney disease (CKD) patients have an increased risk of morbidity and mortality following SARS-CoV-2 infection. Vaccination in these is prioritized, monitoring the immune response paramount to define further vaccination strategies. This prospective study included a cohort 100 adult CKD patients: 48 with transplant (KT) 52 on hemodialysis without prior COVID-19. The were assessed for humoral cellular responses after four months anti-SARS-CoV-2 primary two-dose scheme (CoronaVac or...
The tigecycline susceptibility of six different Enterobacteriaceae strains with reported high MICs was determined in quintuplicate by four methodologies using Mueller-Hinton agar and broth from manufacturers. Etest were a >or=1-fold dilution lower than those microdilution dilution, the highest modal values given dilution. obtained Oxoid medium, lowest inhibition zone (disc diffusion) bioMérieux media. Difco or Merck
The tolerance of the extremophile, Deinococcus radiodurans , to numerous oxidative stresses makes it ideal for bioremediation applications, but many tools necessary metabolic engineering are lacking in this organism compared model bacteria. Although native and engineered promoters have been used drive gene expression protein production D. very few well characterized. Informed by bioinformatics, study expands repertoire well-characterized via thorough characterization eight putative with...
The benefit of autologous platelet-rich plasma (PRP) treatment is still under discussion. Variations in PRP products, consequence the lack a standardised protocol for multitude commercially available blood separation systems and knowledge optimal composition or its suitability proposed indication are some reasons behind clinical inconsistencies. impact inter-subject variability has received less attention comparison. purpose this study was to determine variability, based on age, concentrates...
Satellite cells (SCs) are myogenic progenitors responsible for skeletal muscle regeneration and maintenance. Upon activation, SCs enter a phase of robust proliferation followed by terminal differentiation. Underlying this progression, the sequential expression regulatory transcription factors (MRFs) downregulation factor paired box gene 7 (Pax7) key steps regulating SC fate. In addition to transcriptional regulation, post-translational control Pax7 MRFs provides another layer spatiotemporal...
Streptococcus pneumoniae, pyogenes and Haemophilus influenzae are bacteria present in the nasopharynx as part of normal flora. The ecological equilibrium can be disrupted by presence antibiotics.A computerized two-compartment pharmacodynamic model was used to explore beta-lactam effects on evolution over time a bacterial load containing common pharyngeal isolates simulating free serum concentrations obtained with amoxicillin (AMX) 875 mg tid, amoxicillin/clavulanic acid (AMC) 875/125 tid...
To investigate β-lactam effects on Streptococcus pneumoniae–mixed cultures, a computerized pharmacodynamic model simulating over 24-hr concentrations obtained after several regimens was used. Strain 1 (no penicillin binding protein [PBP] mutations) and strain 2 (mutation in pbp1a) were penicillin/amoxicillin susceptible. 3 (mutations pbp1a, pbp2x, pbp2b) 4 pbp2b [10 changes]) resistant. Initial inoculum approximately 6 × 106 CFU (colony forming units)/ml (with 1:1:1:1 proportion of each...
ObjectivesTo investigate the bactericidal activity, against Haemophilus influenzae strains exhibiting different resistance phenotypes, of simulated serum concentrations obtained in humans after administration 400 mg cefditoren twice daily, 500 cefuroxime 875/125 co-amoxiclav daily or three times daily.