A5081432813- Antibiotics Pharmacokinetics and Efficacy
- Pneumonia and Respiratory Infections
- Antibiotic Resistance in Bacteria
- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Antifungal resistance and susceptibility
- Infective Endocarditis Diagnosis and Management
- Bacterial biofilms and quorum sensing
- Antibiotic Use and Resistance
- Fungal Infections and Studies
- Oral microbiology and periodontitis research
- Pneumocystis jirovecii pneumonia detection and treatment
- Bacterial Infections and Vaccines
- Nail Diseases and Treatments
- Advanced Drug Delivery Systems
- Liver Disease Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Orthopedic Infections and Treatments
- Medical and Biological Ozone Research
- Pharmaceutical studies and practices
- Cystic Fibrosis Research Advances
- Dental Radiography and Imaging
- Nosocomial Infections in ICU
- Otolaryngology and Infectious Diseases
McMaster University
2025
Bellvitge University Hospital
2024
California Institute of Behavioral Neurosciences and Psychology (United States)
2021
Khyber Medical College
2021
Universidad Complutense de Madrid
2005-2017
Asociación Española de Psiquiatría del Niño y el Adolescente
2006
Complejo Hospitalario Universitario de Granada
2000
Universidad de Navarra
1992
Universidad de Extremadura
1987
Linezolid resistance mediated by the cfr gene represents a global concern due to its dissemination among multiresistant nosocomial pathogens such as MRSA and Enterococcus. In present work, we have evaluated in vitro transmission of pSCFS7-like plasmids from two Staphylococcus epidermidis ST2 strains (SE45 SE50) isolated Spanish hospitals, clinical Enterococcus spp. isolates obtained Japan, country which has not been detected yet. We also investigated alternative mechanisms horizontal...
The timely enrollment of study participants is critical to the success clinical trials. Understanding factors that contribute patients’ decision participate in trials involving online cognitive behavioural therapy for pain management should prove helpful optimize design protocols. Fracture patients from an orthopaedic clinic who declined Cognitive Optimize Post-operative rEcovery (COPE) trial were asked complete a Research Participation Questionnaire them about their previous experiences...
In order to explore the pharmacodynamic need for continuous versus intermittent (three times a day) administration of ceftazidime in critically ill patients, pharmacokinetic computerized device was used simulate concentrations human serum after 6 g/day.Efficacy measured as capability simulated over time reduce initial inoculum against four strains Pseudomonas aeruginosa. MICs matched NCCLS breakpoints: one susceptible strain (MIC = 8 mg/L), two intermediate 16 mg/L) and resistant 32 mg/L)....
ObjectivesTo explore serum and tissue pharmacodynamics of linezolid versus vancomycin against methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates with different MBC/MIC ratios.
To compare different methods for the identification and determination of susceptibility to penicillin methicillin Staphylococcus lugdunensis.Seventeen clinical isolates S. lugdunensis (identified by PCR amplification sequencing rpoB gene) were studied using ATB32-Staph, Crystal, Vitek 2 Wider commercial systems. The clumping factor test tube coagulase also performed. Beta-lactamase production was chromogenic methods. Methicillin resistance phenotypically MRSA slide latex agglutination test,...
Bactericidal activity depends on antibiotic-bacteria couples, resistance phenotype and theoretically protein binding. This work explores the influence of binding bactericidal two antibiotics, daptomycin versus vancomycin, that exhibit, respectively, different C(max) (56 25.5 mg/L), (91.7% 36.9%) thus theoretical free-drug fractions (4.7 16.1 mg/L).The effect presence physiological concentrations human albumin (4 g/dL) or serum (90%) was studied against Gram-positive isolates with troublesome...
Aims: The aim of this study was to analyse the nucleotide sequences regions encoding penicillin-binding domains pbp1A, pbp2B and pbp2X genes murM alleles from 14 selected amoxicillin-resistant Streptococcus pneumoniae isolates (MICs 8–16 mg/L) obtained in Spain.
ObjectivesAttempts to interpret antibiotic pharmacodynamics using reported protein binding may underestimate true activity. To elucidate this issue we examined bacterial killing kinetics at cefditoren concentrations equal Cmax in the presence of 90% human serum or albumin physiological concentrations.
ABSTRACT Analysis of the pulsed-field gel electrophoretic profiles 82 pneumococcal isolates with reduced susceptibility to ciprofloxacin (RSC) and 90 co-occurring susceptible indicates a considerable genetic diversity among RCS points close relation between two groups. This finding suggests that pneumococci emerge through independent mutational events.
Background Specific antibodies are likely to be present before S. pneumoniae infection. We explored cefditoren (CDN) total and free values of serum concentrations exceeding the MIC (t>MIC) related efficacy in a mice sepsis model, effect specific gammaglobulins on in-vitro phagocytosis in-vivo efficacy. Methodology/Principal Findings used three pneumococcal isolates (serotype, CDN): Strain 1 (6B, µg/ml), 2 (19F, µg/ml) 3 (23F, 4 µg/ml). Hyperimmune (HS) was obtained from immunized with...
Exposure times and serum concentrations in humans after treatment with multiple doses of fluconazoie have been studied to measure: (a) the postantifungal effect (PAFE) on Candida albicans, at two drug presence or absence 10% human serum; (b) activity low yeasts previously exposed fluconazole without (c) pretreatment fungicidal leucocytes against C. albicans. Fluconazole showed no PAFE albicans between —0.1 —0.7 h, but when assays were performed serum, concentration dependent PAFEs obtained...
ABSTRACT A dose-ranging study to investigate the in vivo effects of presence specific antibodies on efficacy β-lactam treatment sepsis caused by Streptococcus pneumoniae (non-β-lactam-susceptible serotype 6B isolate) was performed with a BALB/c mouse model. Hyperimmune serum obtained from mice immunized heat-inactivated strain. The rate mortality 100% nontreated animals absence antibodies. single injection one-half or one-quarter dilution hyperimmune produced 60 40% survival rates. In...
Objectives: To investigate the bactericidal activity against Streptococcus pneumoniae of simulated amoxicillin serum concentrations obtained in humans after 2000/125 mg sustained-release (SR) and 875/125 co-amoxiclav administered twice three times a day, respectively.
One important feature of the major opportunistic human pathogen Staphylococcus aureus is its extraordinary ability to rapidly acquire resistance antibiotics. Genomic studies reveal that S. carries many virulence and genes located in mobile genetic elements, suggesting horizontal gene transfer (HGT) plays a critical role evolution. However, full detailed description methodology used study HGT still lacking, especially regarding natural transformation, which has been recently reported this...
Abstract Antimicrobial resistance is universally recognized as a major problem. A European survey was established to monitor the activity of widely used oral antibiotics against common respiratory tract pathogens. Studies were conducted in Italy, Spain and Austria patterns among Streptococcus pneumoniae, Haemophilus influenzae, Moraxella catarrhalis, pyogenes, Staphylococcus aureus Klebsiella pneumoniae amoxicillin, co-amoxiclav, penicillin, cefaclor, cefadroxil, cefalexin, cefprozil,...
Objectives: High-level penicillin resistance in Streptococcus pneumoniae requires extensive re-modulation of the penicillin-binding proteins (PBPs), and murM gene function is also required for expression resistance. In this work, we determined whether specific changes PBPs were associated with MurM variants.
Streptococcus pneumoniae, pyogenes and Haemophilus influenzae are bacteria present in the nasopharynx as part of normal flora. The ecological equilibrium can be disrupted by presence antibiotics.A computerized two-compartment pharmacodynamic model was used to explore beta-lactam effects on evolution over time a bacterial load containing common pharyngeal isolates simulating free serum concentrations obtained with amoxicillin (AMX) 875 mg tid, amoxicillin/clavulanic acid (AMC) 875/125 tid...
To investigate β-lactam effects on Streptococcus pneumoniae–mixed cultures, a computerized pharmacodynamic model simulating over 24-hr concentrations obtained after several regimens was used. Strain 1 (no penicillin binding protein [PBP] mutations) and strain 2 (mutation in pbp1a) were penicillin/amoxicillin susceptible. 3 (mutations pbp1a, pbp2x, pbp2b) 4 pbp2b [10 changes]) resistant. Initial inoculum approximately 6 × 106 CFU (colony forming units)/ml (with 1:1:1:1 proportion of each...