A5084723099- MicroRNA in disease regulation
- Extracellular vesicles in disease
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- RNA Research and Splicing
- Cytokine Signaling Pathways and Interactions
- Circular RNAs in diseases
- Asthma and respiratory diseases
- Enzyme Production and Characterization
- Microbial Natural Products and Biosynthesis
- interferon and immune responses
- Biofuel production and bioconversion
- Lipid Membrane Structure and Behavior
- Viral Infections and Vectors
- Microbial Metabolic Engineering and Bioproduction
- S100 Proteins and Annexins
- Acute Myeloid Leukemia Research
- RNA and protein synthesis mechanisms
- Nanopore and Nanochannel Transport Studies
- Neonatal Respiratory Health Research
- TGF-β signaling in diseases
- Caveolin-1 and cellular processes
- Bone Metabolism and Diseases
- Molecular Biology Techniques and Applications
- Cell Adhesion Molecules Research
University Medical Center Hamburg-Eppendorf
2024-2025
University Cancer Center Hamburg
2024-2025
Universität Hamburg
2024-2025
Technical University of Darmstadt
2016-2024
Goethe University Frankfurt
2016-2020
Small extracellular vesicles (sEV) hold enormous potential as biomarkers, drug carriers, and therapeutic agents. However, due to previous limitations in the phenotypic characterization of sEV at single vesicle level, knowledge cell type-specific signatures remains sparse. With introduction next-generation analysis devices, such single-particle interferometric reflectance imaging sensor (SP-IRIS)-based ExoView R100 platform, analyses are now possible. While tetraspanins CD9, CD63, CD81 were...
Extracellular vesicles (EVs) are membrane structures enclosed by a lipid bilayer that released into the extracellular space all types of cells. EVs involved in many physiological processes transporting biologically active substances. Interest for diagnostic biomarker research and therapeutic drug delivery applications has increased recent years. The realization full potential is currently hampered lack suitable technology isolation purification downstream pharmaceutical applications. Anion...
Rheumatoid arthritis (RA) is a chronic autoimmune disease characterized by synovial inflammation and joint destruction. Cell-derived small extracellular vesicles (sEV) mediate cell-to-cell communication in the microenvironment carrying microRNAs (miRs), class of non-coding RNAs. Herein, we report that sEV from fluid promote osteoclast differentiation which attributed to high levels miR-574-5p. Moreover, demonstrate for first time enhanced maturation mediated Toll-like receptor (TLR) 7/8...
Extracellular vesicles (EVs) act as carriers of biological information from tumors to the bloodstream, enabling detection circulating tumor material and tracking disease progression. This is particularly crucial in glioblastoma, a highly aggressive heterogeneous that challenging monitor. Using imaging flow cytometry (IFCM), we conducted an immunophenotyping analysis eight glioma-associated antigens tetraspanins plasma EVs 37 newly diagnosed glioblastoma patients (pre- post-surgery), 11...
Abstract Intercellular communication plays an essential role in lung cancer (LC). One of the major players cell‐cell‐communication is small extracellular vesicles (sEV). SEV trigger various biological responses by transporting cellular cargo to target cells. sEV component are microRNAs (miRs), whose transport has recently attracted increasing research interest. We report that prostaglandin E 2 (PGE ), a key inflammatory lipid mediator, specifically induces sorting miR‐574‐5p A549 and 2106T...
MicroRNAs (miRs) are important posttranscriptional regulators of gene expression. Besides their well-characterized inhibitory effects on mRNA stability and translation, miRs can also activate In this study, we identified a novel noncanonical function miR-574-5p. We found that miR-574-5p acts as an RNA decoy to CUG RNA-binding protein 1 (CUGBP1) antagonizes its function. MiR-574-5p induces microsomal prostaglandin E synthase-1 (mPGES-1) expression by preventing CUGBP1 binding 3′UTR, leading...
Abstract UPF1 is a key player in nonsense mediated mRNA decay (NMD) but also involved posttranscriptional gene regulation. In this study we found that regulates the expression of genes with functions inflammation and myeloid cell differentiation via hnRNP E2. The majority UPF1-regulated identified monocytic cells contain binding site for E2 within 5′ UTR located introns acting here as splicing regulator. We miRNA-328 which significantly induced during acts independently from its silencing...
Extracellular microRNAs (miRs) have been proposed as important blood-based biomarkers for several diseases. Contrary to proteins and other RNA classes, miRs are stable easily detectable in body fluids. In this respect, represent a perfect candidate minimal invasive which can hopefully become complement histological examinations of tumor tissue. Despite the high number miR biomarker studies, specificity reproducibility these studies is missing. Therefore, standardization pre-analytical...
Neuroblastoma is the most common extracranial solid tumor in childhood and arises from neural crest cells of developing sympathetic nervous system. Prostaglandin E 2 (PGE ) has been identified as a key pro-inflammatory mediator microenvironment (TME) that promotes neuroblastoma progression. We report interaction between microRNA miR-574-5p CUG-binding protein 1 (CUGBP1) induces expression microsomal prostaglandin synthase (mPGES-1) cells, which contributes to PGE biosynthesis. turn...
MicroRNAs (miRs) are one of the most important post-transcriptional repressors gene expression. However, miR-574-5p has recently been shown to positively regulate expression microsomal prostaglandin E-synthase-1 (mPGES-1), a key enzyme in E2 (PGE2) biosynthesis, by acting as decoy RNA-binding protein CUG-RNA binding 1 (CUGBP1) human lung cancer. MiR-574-5p exhibits oncogenic properties and promotes tumor growth vivo via induction mPGES-1 derived PGE2 synthesis. In mass spectrometry-based...
Leukotrienes (LTs) are inflammatory mediators that play a pivotal role in many diseases like asthma bronchiale, atherosclerosis and various types of cancer. The key enzyme for generation LTs is the 5-lipoxygenase (5-LO). Here, we present novel putative protein isoform human 5-LO lacks exon 4, termed 5-LOΔ4, identified cells lymphoid origin, namely Burkitt lymphoma cell lines Raji BL41 as well primary B T cells. Deletion 4 does not shift reading frame therefore mRNA subjected to non-mediated...
MicroRNAs (miRs) are small noncoding RNAs which control the expression of target genes by either translational repression or RNA degradation, known as canonical miR functions. The recent discovery that miR-328 has a noncanonical function and can activate gene antagonizing activity heterogeneous ribonuclear protein E2 (hnRNP E2) opens an unexplored exciting field regulation. global importance such is not yet known. In order to achieve better understanding new activity, we performed...
Abstract Circulating microRNAs (miRs) represent promising diagnostic and prognostic biomarkers for various diseases. Despite the high number of biomarker studies, results these studies are hardly reproducible. This makes it difficult to transfer into clinical application. In this context, acceptance criteria quantification miRs by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR) may help standardize miR analysis improve reproducibility.