A5040338725- Mycotoxins in Agriculture and Food
- Ruminant Nutrition and Digestive Physiology
- Pesticide Residue Analysis and Safety
- Pharmaceutical and Antibiotic Environmental Impacts
- Spectroscopy and Chemometric Analyses
- Antibiotics Pharmacokinetics and Efficacy
- Wheat and Barley Genetics and Pathology
- Soil Carbon and Nitrogen Dynamics
- Agronomic Practices and Intercropping Systems
- Soil Geostatistics and Mapping
- Risk Perception and Management
- Analytical Chemistry and Chromatography
- Veterinary medicine and infectious diseases
- Molecular Biology Techniques and Applications
- Advanced Chemical Sensor Technologies
- Plant and fungal interactions
- Plant Toxicity and Pharmacological Properties
- Agricultural Productivity and Crop Improvement
- Agricultural Economics and Policy
- Plant Pathogens and Fungal Diseases
- Forest Management and Policy
- Agriculture, Soil, Plant Science
- Risk and Safety Analysis
- Listeria monocytogenes in Food Safety
- Microbial Natural Products and Biosynthesis
Swedish Veterinary Agency
2008-2020
University of Bonn
2007
Canadian Food Inspection Agency
2007
Berufsgenossenschaft für Gesundheitsdienst und Wohlfahrtspflege
2003
Swedish University of Agricultural Sciences
1983-1989
Abstract Insoluble bound phenolic acids were analysed by g.l.c. and h.p.l.c. in eight abraded fractions of barley grain. Vanillic, p ‐coumaric, ferulic diferulic identified all the fractions. Ferulic ‐coumaric quantitatively most important. The outer layers, comprising husks, pericarp, testa aleurone cells, contained highest concentrations total (0.6–0.9%) while their considerably lower endosperm layers (0.1% or less). Calculated on basis cell wall content fractions, concentration acid was...
Fourier transform near-infrared spectroscopy (FT-NIR) was used for rapid and non-invasive analysis of deoxynivalenol (DON) in durum common wheat. The relevance using ground wheat samples with a homogeneous particle size distribution to minimize measurement variations avoid DON segregation among particles different sizes established. Calibration models wheat, + samples, <500 µm, were obtained by partial least squares (PLS) regression an external validation technique. Values root mean square...
Abstract Lucerne (alfalfa) was harvested at different stages of maturity and the plants classified according to a phenological scale. The main class separated into anatomical parts analysed for content extractives crude protein in‐vitro degradability. Whole crop samples were also polysaccharide residues, Klason lignin degradability residues. composition plant changed during maturation with an increase stem fraction from 18.5% 50.7% dry matter corresponding decrease in leaf 72.9% 18.4%...
Fusarium culmorum is one of the most important fungal plant pathogens that causes diseases on a wide diversity cereal and non-cereal crops. We report herein for first time genome sequence F. strain PV its associated secondary metabolome plays role in interaction with other microorganisms contributes to pathogenicity plants. The revealed presence two terpene synthases, trichodiene longiborneol synthase, which generate an array volatile terpenes. Furthermore, we identified gene clusters,...
Fusarium mycotoxins such as DON and ZEN are commonly occurring in both cereal grains straw particularly temperate regions. Pigs among the most sensitive animals to exposure of these mycotoxins. Straw is used bedding material thus may give a significant contribution pigs. The objective present study was screen cereals (straw) produced at Swedish pig farms for presence DON, ZEN, T-2 HT-2 toxins. 188 samples from 2011 2012 were sampled analysed, where almost ubiquitous with 89% being...
Aflatoxicosis is a major problem associated with poultry production in the tropics. We tested ability of 3 low-cost and locally available adsorbents (activated charcoal, bentonite, fuller’s earth) to detoxify feeds contaminated aflatoxin. Bentonite was most effective adsorbent lowered total aflatoxin concentration from 120 ± 38 μg/kg 15 5.0 μg/kg. The treatments resulted 63%–100% weight increase birds, compared birds fed untreated, aflatoxin-containing feed. number white blood cells samples...
Sampling of straw bales from wheat, barley, and oats was carried out after harvest showing large variations in deoxynivalenol (DON) zearalenone (ZEN) levels. In the wheat field, DON detected all samples with an average concentration 976 μg/kg a median 525 μg/kg, while four bales, concentrations were above 3000 μg/kg. For ZEN, more uniform 11 The barley positive for 449 three 800 oat straw, 6719 lowest at 2614 eight 8000 ZEN contamination 53 highest 219 Oat another field showed 16,382 on 153...
A low cost strategy for objective and rapid selection of soil samples from a large population was evaluated. The purpose the to retain maximum original variation in important properties with only small samples. evaluation made emphasis on clay content, organic matter, cation exchange capacity, base saturation, all which are factors biochemical activities and, therefore, fertility. involved use near infrared (NIR) spectroscopy combined principal component analysis (PCA). 2-nm interval...
The use of electronic gas sensors and near-infrared spectroscopy (NIR) to monitor the dynamics in a biogas process was evaluated using multivariate data analysis. digester, completely stirred 8 l tank reactor fed with mixture cellulose, albumin minerals, exposed an overload glucose after which monitoring sensor responses, NIR spectra as well traditional chemical variables analysis microbial community structure were done. responses from array consisting MOS MOSFET-sensors correlated against...
A collaborative study was conducted to evaluate the effectiveness of an immunoaffinity column cleanup liquid chromatography (LC) method for determination aflatoxin B1 in cattle feed at a possible future European regulatory limit (1 ng/g). The test portion extracted with acetone-water (85 + 15), filtered, diluted water, and applied column. washed water remove interfering compounds, purified eluted methanol. Aflatoxin separated determined by reversed-phase (RP-LC) detected fluorescence after...
For environmental, as well agronomic reasons, the turnover of carbon (C) and nitrogen (N) from crop residues, catch crops green manures incorporated into agricultural soils has attracted much attention. It previously been found that C N content in fractions stepwise chemical digestion plant materials constitutes an adequate basis for describing a priori degradability both soil. However, analyses involved are costly and, therefore, unlikely to be used routinely. The aim present work was...
Abstract The contents of extractives, starch, crude protein, non‐starch polysaccharides and Klason lignin were determined in red clover harvested at six different stages maturity. Chemical analysis residues after incubation with rumen liquor vitro revealed that the degradability starch was 100% protein about 80% all harvest times. decreased from 76 to 51% during maturation no degradation noticed. At times xylose least degraded polysaccharide residue followed by glucose rhamnose. During...
The duplicate method for estimating uncertainty from measurement including sampling is presented in the Eurachem/CITAC guide. applicability of this as a tool verifying plans mycotoxins was assessed three case studies with aflatoxin B1 animal feedingstuffs. Aspects considered included strategies obtaining samples contaminated lots, assumptions about distributions, approaches statistical analysis, log10-transformation test data and estimates. results showed that when aggregate are formed by...
Two surveys are presented of straw analysed for naturally occurring chloramphenicol (CAP), a drug banned use in food-producing animals. In the first study, CAP was by LC-MS/MS and detected 37 out 105 samples originating from Netherlands, France, UK, Germany Denmark. The highest level found 6.3 µg kg(-1), average 0.6 kg(-1) median 0.2 kg(-1). second study included method comparison between ELISA survey cereal sampled at farms all areas Sweden. A total 215 were screened subset 26 also...
Abstract BACKGROUND: Elevated levels of aflatoxin M1 were observed in routine checks consumer milk southern Sweden early 2006. A trace‐back study revealed contaminated from several farms, and a total 68 farms banned delivering to dairies for shorter or longer periods. The maximum level single sample an individual farm was 257 ng kg −1 fresh milk. RESULTS: Aflatoxin analyses commercial compound feed that the contamination originated ingredient rice meal, by‐product preparation Basmati human...
Abstract A method using immunoaffinity column chromatography (IAC) and liquid (LC) for determination of zearalenone in cereal grains, animal feed, feed ingredients was collaboratively studied. The test portion is extracted by shaking with acetonitrilewater (90 + 10, v/v) sodium chloride. extract diluted applied to an column, the washed water or phosphatebuffered saline methanolwater (30 70, v/v), eluted methanol. eluate evaporated, residue dissolved mobile phase analyzed reversed-phase LC...
Risk analysis is a structured approach comprising risk assessment, management and communication, where the latter includes communication between assessors managers. In recent decades there has been shift in paradigm from “strict separation of assessment management” to model dialogue an integral part process. However, as REFIT evaluation concluded, still need for closer cooperation coordination. The goals ENCOMRAN project were (i) capture describe interactions within food feed at EU Member...
Abstract An interlaboratory trial for determination of zearalenone (ZON) in baby food and animal feed was conducted. The study involved 39 participants 16 European Union member states, as well Turkey, Uruguay, China, representing a cross-section industry, official control research institutes. method is based on immunoaffinity column cleanup followed by high-performance liquid chromatography using fluorimetry (HPLC-Fl). test portion the sample extracted with methanolwater (75 + 25, v/v)....
Abstract Soluble degraded lignin complexes (LCs) were prepared using a two‐step incubation with buffered rumen fluid, and isolation of the LC from latter liquid phase by filtration, dialysis freeze drying. The yields crude 19 10 g kg −1 initial dry matter for wheat straw ( Triticum arvense L cv Holme) red clover Trifolium pratense Hermes II) stems, respectively. Gel permeation chromatography both LCs showed similar patterns about 95% material having molecular weight 12 000 to 16 when...
A key feature of risk analysis is that assessment and management should be functionally separated. However, the usefulness a may limited if output not designed to help with decisions. The COMRISK project investigated communication between assessors managers. overall goal was identify current practices challenges in managers during process, thus increase improve understanding quality them. Specific actions achieve this aim included reviewing historical food safety cases, analysing requests,...
Abstract Samples of grass and clover swards from leys were collected during one season on different Swedish farms converting to organic farming, in order obtain a survey general practical differences the botanical chemical composition when farming. The samples analysed with traditional methods as well near‐infrared (NIR) spectroscopy inductively coupled plasma analysis. Multivariate data analysis (principal component partial least squares regression) was used evaluate results. It found that...