A5071201949- 3D Printing in Biomedical Research
- Bioenergy crop production and management
- Pancreatic and Hepatic Oncology Research
- Forest Biomass Utilization and Management
- Cancer Cells and Metastasis
- Biofuel production and bioconversion
- Bacterial Genetics and Biotechnology
- Cancer, Hypoxia, and Metabolism
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Ultrasound and Hyperthermia Applications
- Cancer Research and Treatments
- Cancer-related Molecular Pathways
- Pluripotent Stem Cells Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cell Image Analysis Techniques
- Microfluidic and Capillary Electrophoresis Applications
- Forest Management and Policy
- Tendon Structure and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Metal Extraction and Bioleaching
- Microbial Metabolic Engineering and Bioproduction
- Cancer Immunotherapy and Biomarkers
- Urinary and Genital Oncology Studies
- Signaling Pathways in Disease
University of Oxford
2014-2025
Georgia Institute of Technology
2024
Great Lakes Bioenergy Research Center
2021-2024
Princeton University
2021-2024
University of Wisconsin–Madison
2021
CRUK/MRC Oxford Institute for Radiation Oncology
2010-2020
Cancer Research UK
2014-2019
Medical Research Council
2019
Anglo-Norman Text Society
2019
Michigan Medicine
2016-2017
FK506-binding protein like (FKBPL) and its peptide derivative, AD-01, have already shown tumor growth inhibition CD44-dependent antiangiogenic activity. Here, we explore the ability of AD-01 to target CD44-positive breast cancer stem cells (BCSC).Mammosphere assays flow cytometry were used analyze effect FKBPL overexpression/knockdown treatment ± other anticancer agents on BCSCs using cell lines (MCF-7/MDA-231/ZR-75), primary patient samples, xenografts. Delays in initiation evaluated vivo....
This paper proposes an integrated stochastic mixed-integer linear programming model for biofuel supply chain and landscape design optimization that considers the interactions between uncertainty in biomass yield, spatially explicit feedstock availability, configuration, operational decisions, system's environmental impact. By modeling crop establishment fertilization as strategic decisions made before realization of uncertainty, solutions identify configurations better suited to mitigate...
Abstract Introduction: Culture of autologous patient CAR-T cells with organotypic tumor slices used to determine efficacy a novel anti-5T4 cell therapy in pancreatic ductal adenocarcinoma (PDAC). Methods: 5T4 expression was analyzed using human PDAC tissue and healthy tissue. Immunohistochemistry (IHC) fixed flow cytometry disaggregated samples were assess total surface protein respectively. In vitro testing carried out impedence-based cytotoxicity assays healthy-donor derived lines. Tumor...
The DNA sequence was determined for the cloned Thiobacillus ferrooxidans nifH and part of nifD genes. A putative T. promoter identified whose sequences showed perfect consensus with those Klebsiella pneumoniae nif promoter. Two upstream activator were also identified. amino acid deduced from sequence. In a comparison eight other nitrogen-fixing microbes, Rhizobium sp. isolated Parasponia andersonii greatest homology (74%) Clostridium pasteurianum (nifH 1) least (54%). Fe proteins, japonicum...
Abstract The large-scale production of cellulosic biofuels would involve spatially distributed systems including biomass fields, logistics networks and biorefineries. Better understanding the interactions between landscape-related decisions design biorefineries with carbon capture storage (CCS) in a supply chain context is needed to enable efficient systems. Here we analyse cost greenhouse gas mitigation potential for biofuel chains US Midwest using realistic explicit land availability crop...
The Pseudomonas derived sigma(54)-dependent regulators DmpR and XylR control the expression of genes involved in catabolism aromatic compounds. Binding to distinct, nonoverlapping groups effectors controls activities these transcriptional activators. Previous work has a common mechanistic model for two which effector binding by N-terminal 210 residues (the A-domain) protein relieves repression an intrinsic ATPase activity essential its transcription-promoting property allows productive...
The Pseudomonas ‐derived σ 54 ‐dependent regulator DmpR has an amino‐terminal A‐domain controlling the specificity of activation by aromatic effectors, a central C‐domain mediating ATPase activity essential for transcriptional and carboxy‐terminal D‐domain involved in DNA binding. In presence protein promotes transcription from −24, −12 Po promoter expression specialized (methyl)phenol catabolic enzymes. Previous analysis led to model which acts as interdomain repressor DmpR's promoting...
Abstract Pharmaceutical research requires pre-clinical testing of new therapeutics using both in-vitro and in-vivo models. However, the species specificity non-human models inadequate recapitulation physiological conditions are intrinsic weaknesses. Here we show that perfusion is a vital factor for engineered human tissues to recapitulate key aspects tumour microenvironment. Organotypic culture explants were allowed grow long-term (14–35 days) phenotypic features perfused microtumours...
Abstract Background Pancreatic cancer has a very poor prognosis. Biomarkers that may help predict or diagnose pancreatic lead to earlier diagnosis and improved survival. Methods The prospective China Kadoorie Biobank (CKB) recruited 512 891 adults aged 30–79 years during 2004–08, recording 702 incident cases of 9 follow-up. We conducted case-subcohort study measuring 92 proteins in 610 subcohort 623 individuals, using the OLINK immuno-oncology panel stored baseline plasma samples. Cox...
The σ54-dependent DmpR activator regulates transcription of the dmp operon that encodes enzymes for catabolism (methyl)phenols. is expressed constitutively, but its transcriptional promoting activity controlled positively in direct response to presence aromatic pathway substrates (effectors). has a distinct domain structure with amino-terminal A-domain controlling specificity activation regulator by effectors (signal reception), central C-domain mediating an ATPase essential activation, and...
Although usually studied separately, integrating the landscape design problem with biomass supply chain network (SCND) has potential to identify farm fuel production systems that work cohesively and are holistically sustainable. Expanding SCND system boundary include presents spatially explicit modeling challenges, but also allows better control of feedstock supply, a more accurate forecast greenhouse gas (GHG) emissions, consideration alternative sustainability metrics, ability account for...
The Pseudomonas derived ς54-dependent DmpR activator regulates transcription of the (methyl)phenol catabolic dmp-operon. is constitutively expressed, but its transcriptional promoting activity positively controlled in direct response to presence multiple aromatic effectors. Previous work has led a model which effector binding by amino-terminal region protein relieves repression an intrinsic ATPase essential for property. Here, we address whether observed differences potencies effectors (i)...
Antiangiogenic therapy for cancer is a strategy targeted at tumour vasculature, often in combination with conventional cytotoxicity treatments. Animal testing still the most common method used evaluating efficacy of new drugs but tissue-engineered vitro models are becoming more acceptable replacing and reducing use animals anti-cancer drug screening. In this study, 3D co-culture model human endothelial cells ovarian was developed. This has potential to mimic interactions between cells. The...
Abstract Approximately 75% of bladder cancers are non‐muscle invasive (NMIBC). Of these, up to 53% cases progress life‐threatening muscle‐invasive cancer (MIBC). Patients with high‐grade stage T1 (HGT1) NMIBC frequently undergo radical cystectomy (RC), although this represents overtreatment for many. Identification progressors versus non‐progressors could spare unnecessary treatment. Recent studies have confirmed that urothelial carcinoma is composed two main molecular subtypes, basal and...