A5046265360- Microfluidic and Capillary Electrophoresis Applications
- Microfluidic and Bio-sensing Technologies
- Innovative Microfluidic and Catalytic Techniques Innovation
- Advanced Biosensing Techniques and Applications
- Biosensors and Analytical Detection
- Single-cell and spatial transcriptomics
- Advanced biosensing and bioanalysis techniques
- Electrowetting and Microfluidic Technologies
- Monoclonal and Polyclonal Antibodies Research
- 3D Printing in Biomedical Research
- Cell Image Analysis Techniques
- Advanced Proteomics Techniques and Applications
- Cellular Mechanics and Interactions
- Advanced Fluorescence Microscopy Techniques
- Nanopore and Nanochannel Transport Studies
- Salivary Gland Disorders and Functions
- Viral Infectious Diseases and Gene Expression in Insects
- Mass Spectrometry Techniques and Applications
- RNA and protein synthesis mechanisms
- Ocular Surface and Contact Lens
- Photoacoustic and Ultrasonic Imaging
- Oral microbiology and periodontitis research
- Infection Control and Ventilation
- Image Enhancement Techniques
- Protein purification and stability
University of California, Berkeley
2016-2025
Chan Zuckerberg Initiative (United States)
2018-2024
University of California, San Francisco
2008-2021
Berkeley College
2015-2021
Chan Zuckerberg Biohub San Francisco
2019-2021
University of California System
2017-2020
Graduate Theological Union
2008-2016
Bioengineering Center
2008-2015
Sensors (United States)
2010
Sandia National Laboratories California
2004-2009
The present work is an analytical and experimental study of electroosmotic flow (EOF) in cylindrical capillaries with nonuniform wall surface charge (zeta-potential) distributions. In particular, this investigates perturbations open that are due to induced pressure gradients resulting from axial variations the zeta-potential. inquiry focuses on under a uniform applied field EOF-suppressing polymer adsorbed onto various fractions total capillary length. This fractional EOF suppression was...
At present, point-of-care (POC) diagnostics typically provide a binary indication of health status (e.g., home pregnancy test strip). Before anticipatory use for assessment complex diseases becomes widespread, development sophisticated bioassays capable quantitatively measuring disease biomarkers is necessary. Successful translation new into clinical settings demands the ability to monitor both onset and progression disease. Here we report on POC diagnostic that enables rapid quantitation an...
Periodontitis is the major cause of tooth loss in adults and linked to systemic illnesses, such as cardiovascular disease stroke. The development rapid point-of-care (POC) chairside diagnostics has potential for early detection periodontal infection progression identify incipient reduce health care costs. However, validation effective requires identification verification biomarkers correlated with progression. This clinical study sought determine ability putative host- microbially derived...
Circulating tumour cells (CTCs) are rare found in the circulatory system of certain cancer patients. The clinical and functional significance CTCs is still under investigation. Protein profiling would complement recent advances enumeration, transcriptomic genomic characterization these help define their characteristics. Here we describe a microfluidic western blot for an eight-plex protein panel individual derived from estrogen receptor-positive (ER+) breast precision handling analysis...
The purpose of this study was to determine the role saliva-derived biomarkers and periodontal pathogens during disease progression (PDP). One hundred human participants were recruited into a 12-month investigation. They seen bi-monthly for saliva clinical measures bi-annually subtraction radiography, serum plaque biofilm assessments. Saliva analyzed with protein arrays 14 pro-inflammatory bone turnover markers, while qPCR used detection biofilm. A hierarchical clustering algorithm group...
Chip-based microcolumn separation systems often require serpentine channels to achieve longer lengths within a compact area. However, analyte bands traveling through curved experience an increased dispersion that can reduce the benefit of channel length. This paper presents analytical solutions for dispersions, numerical models minimizing in microchannel turns, and experiments used validate demonstrate effectiveness dispersion-reduction schemes. An solution geometric caused by constant...
We have developed an acrylic microfluidic device that sequentially couples liquid-phase isoelectric focusing (IEF) and free solution capillary electrophoresis (CE). Rapid separation (<1 min) preconcentration (73×) of species were achieved in the initial IEF dimension. Using full-field fluorescence imaging, we observed nondispersive mobilization velocities on order 20 μm/s during characterization step. This transport behavior allowed controlled electrokinetic focused sample bands to a channel...
The potential of integration functions in microfluidic chips is demonstrated by implementing on-chip preconcentration proteins prior to protein sizing sodium dodecyl sulfate−polyacrylamide gel electrophoresis (SDS−PAGE). Two polymeric elementsa thin (∼50 μm) size exclusion membrane for and a longer (∼cm) porous monolith sizingwere fabricated situ using photopolymerization. Contiguous placement the two elements channels microchip enabled simple zero dead volume with SDS−PAGE. was polymerized...
Rapid, quantitative Western blotting is a long-sought bioanalytical goal in the life sciences. To this end, we describe assay conducted single glass microchannel under purely electronic control. The μWestern blot comprised of multiple steps: sample enrichment, protein sizing, immobilization (blotting), and situ antibody probing. validate microfluidic assay, apply to analyses human sera (HIV immunoreactivity) cell lysate (NFκB). Analytical performance advances are achieved, including: short...
Cells are essential to understanding health and disease, yet traditional models fall short of modeling simulating their function behavior. Advances in AI omics offer groundbreaking opportunities create an virtual cell (AIVC), a multi-scale, multi-modal large-neural-network-based model that can represent simulate the behavior molecules, cells, tissues across diverse states. This Perspective provides vision on design how collaborative efforts build AIVCs will transform biological research by...
A dearth of protein isoform-based clinical diagnostics currently hinders advances in personalized medicine. well-organized biomarker validation process that includes facile measurement isoforms would accelerate development effective protein-based diagnostics. Toward scalable isoform analysis, we introduce a microfluidic “single-channel, multistage” immunoblotting strategy. The multistep assay performs all steps: separation, immobilization resolved proteins, antibody probing immobilized and...
Intratumor heterogeneity remains a major obstacle to effective cancer therapy and personalized medicine. Current understanding points differential therapeutic response among subpopulations of tumor cells as key challenge successful treatment. To advance our how this is reflected in cell-to-cell variations chemosensitivity expression drug-resistance proteins, we optimize apply new targeted proteomics modality, single-cell western blotting (scWestern), human glioblastoma cell line. acquire...
The ability to probe the protein content of human tear fluid has enormous potential for deepening our understanding ocular and systemic disease pathology enabling novel noninvasive tear-based diagnostic technologies. To overcome current challenges in proteomic measurements, we report on first microfluidic homogeneous immunoassay capable making rapid, quantitative, specific measurements endogenous biomarkers fluid. Lactoferrin (Lf) is a tear-specific biomarker Sjögren's syndrome (SS), serious...
Archived patient-derived tissue specimens play a central role in understanding disease and developing therapies. To address specificity sensitivity shortcomings of existing single-cell resolution proteoform analysis tools, we introduce hybrid microfluidic platform (DropBlot) designed for analyses chemically fixed single cells. DropBlot serially integrates droplet-based encapsulation lysis cells, with on-chip microwell-based antigen retrieval, western blotting target antigens. A water-in-oil...
Pore-gradient microgel arrays enable thousands of parallel high-resolution single-cell protein electrophoresis separations for targets accross a wide molecular mass (25–289 kDa), yet within 1 mm separation distances. Dual crosslinked hydrogels facilitate gel-pore expansion after efficient and uniform immunoprobing. The photopatterned, light-activated, acid-expandable hydrogel underpins analysis, here oncoprotein-related signaling in human breast biopsy. As service to our authors readers,...
Abstract Although immunoassays are the de facto standard for determining subcellular protein localization in individual cells, antibody probe cross-reactivity and fixation artifacts remain confounding factors. To enhance selectivity while providing single-cell resolution, we introduce a western blotting technique capable of separately assaying proteins 14 pL cytoplasm 2 nucleus cells. confer precision fluidic control, describe passive multilayer microdevice that leverages rapid transport...
All human diseases involve proteins, yet our current tools to characterize and quantify them are limited. To better elucidate proteins across space, time, molecular composition, we provide a >10 years of projection for technologies meet the challenges that protein biology presents. With broad perspective, discuss grand opportunities transition science proteomics into more propulsive enterprise. Extrapolating recent trends, describe next generation approaches define, quantify, visualize...
Simultaneous profiling of proteoforms and nucleic acids at the single-cell level, i.e., multi omics, directly links central dogma. However, current approaches are limited in their ability to identify while preserving nucleus for further analysis. This limitation is especially pronounced proteins where present diverse biological functions such as cytokeratin 8 (CK8), which, commonly known its structural role, also involved several diseases. Here, we a western blot (scWB) integrated with...