A5109552590- Prostate Cancer Treatment and Research
- Estrogen and related hormone effects
- Phagocytosis and Immune Regulation
- Molecular Biology Techniques and Applications
- Cancer, Hypoxia, and Metabolism
- Prostate Cancer Diagnosis and Treatment
- Cancer, Lipids, and Metabolism
- Hormonal and reproductive studies
- Cancer Research and Treatments
- Vitamin D Research Studies
- Cancer Genomics and Diagnostics
- Renal and related cancers
- Cancer Cells and Metastasis
- Tissue Engineering and Regenerative Medicine
- Renal cell carcinoma treatment
- Immunotherapy and Immune Responses
- Epigenetics and DNA Methylation
- Cancer-related Molecular Pathways
- 14-3-3 protein interactions
- Cancer-related gene regulation
- Glycosylation and Glycoproteins Research
- Cancer Mechanisms and Therapy
- Retinoids in leukemia and cellular processes
- Pancreatic and Hepatic Oncology Research
- Mass Spectrometry Techniques and Applications
University of California, San Francisco
2019-2025
University of California System
2022-2023
Stanford University
2013-2022
John Wiley & Sons (United States)
2015-2021
Johns Hopkins University
2019-2021
Johns Hopkins Medicine
2019-2021
Sidney Kimmel Comprehensive Cancer Center
2019-2021
Palo Alto University
2009-2016
Stratford University
2011-2015
Stanford Medicine
1987-2015
Cancer-associated fibroblasts (CAFs) are a major cellular component of tumor microenvironment in most solid cancers. Altered metabolism is hallmark cancer, and much the published literature has focused on neoplastic cell-autonomous processes for these adaptations. We demonstrate that exosomes secreted by patient-derived CAFs can strikingly reprogram metabolic machinery following their uptake cancer cells. find CAF-derived (CDEs) inhibit mitochondrial oxidative phosphorylation, thereby...
Insulin-like growth factors (IGFs) are potent mitogens that bind with high affinity and specificity to IGF receptors IGF-binding proteins (IGFBPs). We studied the roles of these three groups in prostate epithelial cells (PEC) primary culture grown under serum-free conditions. Affinity cross-linking IGF-I IGF-II crude membranes prepared from PEC revealed an abundance type 1 no evidence 2 receptors. Western ligand blots conditioned media (CM) demonstrated presence two specific IGFBP bands...
Intraspecific human-human cell hybrids provide a stable model system with which to investigate the genetic control of transformed and tumorigenic phenotypes. Using this it has been shown that these phenotypes are under separate control. Furthermore, phenotype can be complemented by fusion different cells, resulting in nontumorigenic hybrids. This also provides information on differentiated function. Molecular cytogenetic techniques should reveal nature chromosomal neoplastic transformation.
Abstract Calcitriol exhibits antiproliferative and prodifferentiation effects in prostate cancer. Our goal is to further define the mechanisms underlying these actions. We studied established human cancer cell lines primary prostatic epithelial cells showed that calcitriol regulated expression of genes involved metabolism prostaglandins (PGs), known stimulators growth. significantly repressed mRNA protein prostaglandin endoperoxide synthase/cyclooxygenase-2 (COX-2), key PG synthesis enzyme....
Expression of the inflammatory chemokine CCL5 (RANTES) by tumor cells is thought to correlate with progression several cancers. was shown induce breast cancer cell migration, mediated receptor CCR5. A CCR5 antagonist demonstrated inhibit experimental growth. Recently, and mRNA expression reported in prostate (PCa) tissues. Herein, we characterized cultures PCa explored possible functions progression.Quantitative RT-PCR, ELISA, immunohistochemical staining were performed examine lines....
Candidate gene-based studies have identified a handful of aberrant CpG DNA methylation events in prostate cancer. However, profiles not been compared on large scale between tumor and normal prostate, the mechanisms behind these alterations are unknown. In this study, we quantitatively profiled 95 primary tumors 86 benign adjacent tissue samples for their levels at 26,333 CpGs representing 14,104 gene promoters by using Illumina HumanMethylation27 platform. A 2-class Significance Analysis...
Abstract Although numerous studies have implicated vitamin D in preventing prostate cancer, the underlying mechanism(s) remains unclear. Using normal human prostatic epithelial cells, we examined role of mitogen-activated protein kinase phosphatase 5 (MKP5) mediating cancer preventive activities D. Up-regulation MKP5 mRNA by 1,25-dihydroxyvitamin-D3 (1,25D) was dependent on receptor. We also identified a putative positive response element within promoter that associated with receptor...
No AccessJournal of UrologyInvestigative Urology1 Aug 1996Prostate Specific Antigen Cleaves Parathyroid Hormone-Related Protein in the PTH-like Domain: Inactivation PTHrP-Stimulated cAMP Accumulation Mouse Osteoblasts Scott D. Cramer, Zuxiong Chen, and Donna M. Peehl CramerScott Cramer , ChenZuxiong Chen PeehlDonna View All Author Informationhttps://doi.org/10.1016/S0022-5347(01)65919-6AboutFull TextPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints...
Abstract In androgen target tissues, 3α-hydroxysteroid dehydrogenase (3α-HSD) may regulate occupancy of the receptor (AR) by catalyzing interconversion 5α-dihydrotestosterone (5α-DHT) (a potent androgen) and 3α-androstanediol weak androgen). this study, a 3α-HSD cDNA (1170 bp) was isolated from human prostate library. The prostatic encodes 323-amino acid protein with 69.9%, 84.1%, 99.4%, 87.9% sequence identity to rat liver type 1, 2, 3 3α-HSDs, respectively, is member aldo-keto reductase...
Abstract Hepcidin is a circulating peptide hormone made by the liver that central regulator of systemic iron uptake and recycling. Here, we report prostate epithelial cells also synthesize hepcidin, synthesis secretion hepcidin are markedly increased in cancer tissue. Prostatic functions as an autocrine hormone, decreasing cell surface ferroportin, exporter, increasing intracellular retention, promoting survival. Synthesis controlled unique intersection pathways involves BMP4/7, IL6, Wnt,...
Significance Differences in cell–cell interfacial energies can explain how multiple cell types sort into spatially organized tissues. However, this strategy of self-organization is not robust to heterogeneity or changes the that drive correct positioning. Therefore, heterogeneous epithelial tissues such as human mammary and prostate glands use a different strategy. First, disorganized aggregates form an adhesive interface at tissue–ECM boundary provides geometric constraints...
Significance NEPC is a highly aggressive subtype of prostate cancer that increasing in incidence, likely due to use new secondary androgen deprivation therapies. Here, we demonstrate Trop2 significantly elevated CRPC and represents driver metastatic NEPC. overexpression increases tumor growth, drives metastasis neuroendocrine phenotype, PARP1 levels. Inhibition Trop2-driven decreases features, colonization vivo, suggesting inhibitors may represent promising therapeutic strategy for...
Loss of the von Hippel-Lindau (VHL) tumor suppressor is a hallmark feature renal clear cell carcinoma. VHL inactivation results in constitutive activation hypoxia-inducible factors (HIFs) HIF-1 and HIF-2 their downstream targets, including proangiogenic VEGF PDGF. However, antiangiogenic agents inhibitors have limited efficacy cancer therapy due to development resistance. Here we employed an innovative computational platform, Mining Synthetic Lethals (MiSL), identify synthetic lethal...