Abstract Analyses of several databases showed that the lncRNA RNF157 Antisense RNA 1 (RNF157-AS1) is overexpressed in epithelial ovarian cancer (EOC) tissues. In our study, suppressing RNF157-AS1 strikingly reduced proliferation, invasion, and migration EOC cells compared with control cells, while overexpressing greatly increased these effects. By pulldown assays, binding protein immunoprecipitation (RIP) mass spectrometry, was further found to be able bind HMGA1 EZH2 proteins. Chromatin...

Multiple pathogenic Gram-negative bacteria produce the cytolethal distending toxin (CDT) with activity of DNase I; CDT can induce DNA double-strand breaks (DSBs), G2/M cell cycle arrest, and apoptosis in cultured mammalian cells. However, link to vivo tumorigenesis is not fully understood. In this study, 129/SvEv Rag2-/- mice were gavaged wild-type Helicobacter hepatics 3B1(Hh) its isogenic cdtB mutant HhcdtBm7, followed by infection for 10 20 weeks (WPI). HhCDT deficiency did affect cecal...

Abstract Background Circular RNA (circRNA), a class of with covalent closed circular structure that widely existed in serum and plasma, has been considered an ideal liquid biopsy marker many diseases. In this study, we employed microarray qRT-PCR to evaluate the potential circulating circRNAs diagnostic efficacy ovarian cancer. Methods We used explore circRNA expression profile cancer patients’ plasma quantitative real-time (qRT)-PCR approach assessing candidate circRNA’s expression. Then...

The mortality rate of ovarian cancer ranks first among three common gynecological malignant tumors due to insidious onset and lack effective early diagnosis methods. Borderline epithelial tumor (BEOT) is a type low potential that typically associated with better outcomes than cancer. However, BEOTs are easily confused benign (EOTs) similar clinical symptoms specific biomarkers imaging examinations. Notably, small subset will transform into low-grade serous carcinoma poor prognosis....

Timely diagnosis of borderline ovarian tumors (BOTs) is crucial for preserving fertility and function. However, current markers detecting BOTs lack effectiveness. This research aims to identify validate the role small molecular in diagnosing BOTs. Six molecule markers-human epididymis protein 4 (HE4), carbohydrate antigen 125 (CA125), fibrinogen (FIB), D-dimer (DD), platelet (PLT), homocysteine (HCY)-were identified as candidate markers. Candidate were evaluated using receiver operating...

Increasing evidence shows that GSDME is involved in tumor chemotherapy. Lobaplatin an important chemotherapy drug for the treatment of cervical cancer. However, exact mechanism lobaplatin cancer remains unclear. In this study, whether a new has been explored. Cell pyroptosis was measured by Counting Kit-8 and flow cytometry analyses. Western blot analysis used to check proteins expression. The cell viability significantly decreased treatment. Compared with control group, percentage (PI...

WRKY transcription factors constitute one of the largest gene families in plants and are involved many biological processes, including growth development, physiological metabolism, stress response. In earlier studies, family proteins has been extensively studied analyzed plant species. However, information on Acer truncatum not reported. this study, we conducted genome-wide identification analysis A. truncatum, 54 genes were unevenly located all 13 chromosomes highest number was found 5....

Abstract Ovarian cancer (OC) is the second leading cause of gynecological cancer‐related death in women worldwide. N6‐methyladenosine (m 6 A) most abundant internal modification eukaryotic RNA. Human insulin‐like growth factor 2 mRNA‐binding protein (IGF2BP2), an m A reader, can enhance mRNA stability and promote translation by recognizing modifications. Its tumor‐promoting effects have been demonstrated several cancers. However, roles IGF2BP2 OC remain unclear. Here, using methylated RNA...

<h3>Objective</h3> This study investigated the involvement of fibrillar collagen in remodeling extracellular matrices (ECM) and its significant impact on metastasis/invasion epithelial ovarian cancer cells via β1 integrin/phosphatase tensin homolog (PTEN) signaling. <h3>Materials/Methods</h3> Normal surface epithelium tissues (n = 13), 28), cell lines, a 3-dimensional model type I that mimicked pathological ECM vivo were used study. We explored specific mechanisms behind cellular signals...

Purpose: To establish an efficient new risk index for screening patients with endometrial cancer from abnormal vaginal bleeding or discharge. Method: A total of 254 discharge were included in this study. Several candidate markers, including HE4, CA125, CA199, CA153, AFP, CEA, d-dimer, and fibrinogen, employed. was established by binary logistic regression. The diagnostic value the markers assessed a receiver operating characteristic curve, sensitivity, specificity. Results: most valuable...

Abstract: Atorvastatin is a lipid-regulating drug that commonly used in clinical practice and can stabilize plaques. Increasing evidence shows statins have anti–heart failure (HF) effects, but their specific mechanism not clear. The purpose of this study was to investigate the cardioprotective effects atorvastatin on HF rats its mechanism. Continuous intraperitoneal injection 2.5 mg/kg/w doxorubicin for 6 weeks, with cumulative dose 15 mg/kg, induce rat model HF. Then, were treated low-dose...

Background: Previous studies have showed that lycorine can restrain the development of multiple tumor types, containing hepatocellular carcinoma (HCC), but underlying mechanisms remain unknown.Methods: We assessed impact on cancer cell proliferation, migration, colony formation, cycle, and apoptosis.The possible inhibitory effect activity HCC cells was analyzed by RNA-seq, transketolase (TKT) expression in nontumorous tissues detected using RT-PCR.The TKT protein adjacent non-cancerous...

Chicken plasma protein hydrolysate (CPPH) was prepared by trypsin with angiotensin I-converting enzyme (ACE) inhibitory activity of 53.5% ± 0.14% and the degree hydrolysis (DH) 16.22% 0.21% at 1 mg·ml

The objective of the current study was to explore role H19 rs217727 polymorphism in control hepatocellular carcinoma (HCC).The Student's t test, Cox regression, and Kaplan-Meier analyses were used clarify whether played an important development HCC. Real-time polymerase chain reaction (PCR) western-blot analysis carried out measure levels H19, microRNA (miR)-675, FAS-associated death domain (FADD), caspase-8, caspase-3 among CC, CT, TT groups, as well cells transfected with H19/si-H19, or...

Abstract Background The objective of this study was to clarify the molecular pathways involved in hepatitis B virus (HBV)‐induced hepatoblastoma. Method expression factors different signaling (H19, miR‐675, miR‐138, protein tyrosine kinase 2 [PTK2], fas‐associated death domain [FADD], hypoxia‐inducible factor 1‐alpha [HIFIA], focal adhesion [FAK], caspase‐8, and caspase‐3) compared between HBV (+) (−) groups using quantitative real‐time polymerase chain reaction Western blot analysis....

A substantive body of evidence has demonstrated the significant roles circular RNA (circRNA) in cancer. However, contribution dysregulated circRNAs to ovarian cancer (OC) remains elusive. We aim elucidate critical and mechanisms hsa_circ_0020093, which was be downregulated OC tissues our previous study. In this study, we confirmed decreased expression hsa_circ_0020093 cell lines negative correlation between its FIGO stage, abdominal implantation CA125 level patients. Through gain loss...

Abstract Several circRNAs have been reported to be dysregulated in human endothelial cells through sponging miRNAs. Previous reports demonstrated that MPO not only contributed the formation and rupture of cerebral aneurysm but was also correlated with degenerative remodeling predisposition saccular intracranial wall rupture, although its underlying mechanisms remain explored. Microarray screening performed compare differential expression collected from UIAs RIAs patients. Luciferase assays...

In this study, we aimed to study the effect of moxibustion (MOX) on microbiota dysbiosis and macrophage polarization, so as unveil mechanism underlying therapeutic MOX in management spinal cord injury (SCI). SCI animal models were established MOX. Accordingly, it was found that treatment significantly suppressed Ace index Shannon group. Moreover, reduced relative levels Lactobacillales Bifidobacteriales elevated level Clostridiales animals mitigated by The body weight, food intake, energy...

Abstract Introduction Hepatoblastoma is a rare but devastating pediatric liver malignancy. Overexpressed methyltransferase-like 1 (METTL1) methyltransferase that catalyzes essential N7-methylguanosine (m7G) modification of eukaryotic mRNA. Accumulating evidence has revealed the oncogenic potential METTL1. However, whether METTL1 gene polymorphisms confer susceptibility to hepatoblastoma not been reported. This study aimed identify causal relationships between genetic variants this and...

Recently, increasing evidence has displayed that lncRNAs can exhibit crucial function in cancer progression, including lung cancer. LncRNA bladder cancer-associated transcript 1 (BLACAT1) is reported to participate various cancers. The aim of our current study was investigate the BLACAT1 non-small cell progression and functional pathway. Here, we significantly up-regulated tissues comparison adjacent normal tissues, which suggested might act as an oncogene Then, A549 PC9 cells were infected...

Abstract Previous studies have demonstrated that cognitive dysfunction is associated with neurophysiological changes in postpartum period. This study aimed to investigate the intrinsic functional connectivity (FC) pattern within default mode network (DMN) and its associations women without depression revealed by resting-state magnetic resonance imaging (fMRI). Resting-state fMRI scans were acquired from 21 age- education-matched nulliparous women. The posterior cingulate cortex (PCC) was...