A5000927518- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Immune Cell Function and Interaction
- Dental Research and COVID-19
- SARS-CoV-2 detection and testing
- RNA modifications and cancer
- Cancer Genomics and Diagnostics
- Genetics and Neurodevelopmental Disorders
- Epigenetics and DNA Methylation
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Influenza Virus Research Studies
- Autism Spectrum Disorder Research
- Congenital heart defects research
- Tuberculosis Research and Epidemiology
- Cancer Cells and Metastasis
- Pneumonia and Respiratory Infections
- Mycobacterium research and diagnosis
- Animal Disease Management and Epidemiology
- Radiopharmaceutical Chemistry and Applications
- Bee Products Chemical Analysis
- COVID-19 and Mental Health
- Melanoma and MAPK Pathways
- TGF-β signaling in diseases
- T-cell and B-cell Immunology
University of Nottingham
2013-2025
Loughborough University
2025
University of California, San Francisco
2022
Dana-Farber Cancer Institute
2016-2021
Harvard University
2021
Broad Institute
2021
Imperial College London
2006-2013
Asthma UK
2007-2013
Medical Research Council
2013
ABSTRACT Macrophages are abundant in the lower respiratory tract. They play a central role innate response to infection but may also modulate excessive inflammation. Both macrophages and ciliated epithelial cells respond by releasing soluble mediators, leading recruitment of adaptive effector cells. To study lung acute viral infection, we depleted them inhalation clodronate liposomes an established mouse model syncytial virus (RSV) disease. Infection caused immediate local release...
Respiratory syncytial virus (RSV) is the major cause of infantile bronchiolitis and hospitalization. Severe RSV disease associated with development wheezing in later life. In a mouse model delayed effects RSV, age at primary infection determines responses to reinfection adulthood. During infection, neonatal BALB/c mice developed only mild recruited CD8 cells that were defective gamma interferon production. Secondary neonatally primed caused enhanced inflammation profuse lung T-cell...
The long-term control strategy of SARS-CoV-2 and other major respiratory viruses needs to include antivirals treat acute infections, in addition the judicious use effective vaccines. Whilst COVID-19 vaccines are being rolled out for mass vaccination, modest number or development any disease bears testament challenges antiviral development. We recently showed that non-cytotoxic levels thapsigargin (TG), an inhibitor sarcoplasmic/endoplasmic reticulum (ER) Ca2+ ATPase pump, induces a potent...
Homeobox genes are master regulators of cell fate during embryonic development and their expression is altered in cancer. By regulating the balance between proliferation differentiation, they maintain homeostasis normal tissues. Here, we screened homeobox mammary stem cells to establish role transformation breast Using a Genes PCR array, 83 cancer stem/progenitor isolated by flow cytometry. The candidate gene HOXC8 epigenetic regulation was studied DNA methylation miRNA analyses....
Metastasis is a complex and poorly understood process. In pancreatic cancer, loss of the transforming growth factor (TGF)-β/BMP effector SMAD4 correlated with changes in altered histopathological transitions, metastatic disease, poor prognosis. this study, we use isogenic cancer cell lines to identify regulated genes that contribute development colonization. We perform an vivo screen identifying FOSL1 as both target sufficient drive colonization lung. The targeting these early treatment may...
Respiratory syncytial virus (RSV) causes bronchiolitis in young children and common colds adults. There is no licensed vaccine, prophylactic treatment with palivizumab very expensive limited to high-risk infants. Ribavirin used as an antiviral infants immunosuppressed patients, its use due side-effects, toxicity the recipient staff, evidence of marginal clinical efficacy. Therefore, we studied vivo kinetics, protective properties a novel candidate for RSV disease treatment. The drug small...
Hatcheries have been identified as a significant source of Salmonella within poultry production. Consequently, there is need for effective egg disinfection methods that can reduce the pathogen burden while preserving integrity and embryo. The metrics successful method are typically reduction in Total Aerobic Count (TAC) retaining hatching rates. In this study, gas phase hydroxyl-radical process was validated verified hatchery method. based on applying hydrogen peroxide mist combination with...
Abstract Bovine tuberculosis (bTB) is a major disease of cattle in the UK, placing significant economic burden on taxpayer. The causative agent, Mycobacterium bovis , has wide host range, including European badger ( Meles meles) . While badgers have been implicated transmission and maintenance infection areas endemic disease, their role at edge area poorly understood. Here we present data prevalence collected along southern England’s bTB epidemic. Stakeholders across five counties...
CD8 T cells assist in the clearance of respiratory syncytial virus (RSV) infection from lungs. However, disease after RSV is part caused by excessive cell activity, and a balance therefore needed between beneficial harmful cellular immune responses. The chemokine CCL3 (MIP1alpha) produced following broadly chemotactic for both natural killer (NK) cells. We investigated its role disease.CCL3 was biphasically, early (day 1) late 6-7) stages infection. depletion did not alter recruitment to...
Respiratory syncytial virus (RSV) infects most children in the first year of life and is a major single cause hospitalization infants young children. There no effective vaccine, antibody generated by primary neonatal infection poorly protective against reinfection even with antigenically homologous viral strains. Studying immunological basis these observations mice, we found that responses to were low unaffected CD4 depletion, contrast adult which had stronger CD4-dependent responses....
Abstract Respiratory syncytial virus (RSV) is a major cause of respiratory morbidity, resulting in hospitalization for bronchiolitis some infected infants that associated with wheeze later life. Genetic factors are known to affect the severity sequelae after RSV infection, but complexity temporal and genetic effects makes it difficult analyze this response studies man. Therefore, we developed murine model occurring infection early Haplotype-based analysis interstrain differences identified...
ABSTRACT Severe respiratory viral infection in early life is associated with recurrent wheeze and asthma later childhood. Neonatal immune responses tend to be skewed toward T helper 2 (Th2) responses, which may contribute the development of a pathogenic recall response infection. Since neonatal Th2 skewing can modified by stimulation Toll-like receptor (TLR) ligands, we investigated effect exposure CpG oligodeoxynucleotides (TLR9 ligands) prior syncytial virus (RSV) mice. preexposure was...
ABSTRACT Respiratory syncytial virus (RSV) is the main cause of bronchiolitis, major hospitalization infants. An ideal RSV vaccine would be effective for neonates, but immune responses infants differ markedly from those adults, often showing a bias toward T-helper 2 (Th2) and reduced gamma interferon (IFN-γ) production. We previously developed recombinant vectors expressing IFN-γ interleukin-4 (IL-4) that allow us to explore role these key Th1 Th2 cytokines during infection. The aim current...
ABSTRACT Respiratory syncytial virus (RSV) is an important cause of infant morbidity and mortality worldwide increasingly recognized to have a role in the development exacerbation chronic lung diseases. There no effective vaccine, we reasoned that it might be possible skew immune system towards beneficial nonpathogenic responses by selectively priming protective T-cell subsets. We therefore tested recombinant RSV (rRSV) candidates expressing prototypic murine Th1 (gamma interferon [IFN-γ])...
Overexpression of the Human endogenous retrovirus W (HERV-W) group inherited retroviruses has been consistently linked with Multiple Sclerosis (MS). However most studies on this link have focused European genetic groups a very high risk MS and it is not clear that relationship holds for all ethnic groups. This study examined via qPCR RNA expression in peripheral blood HERV-W (the multiple sclerosis associated variant MSRV) patients healthy controls from two different rates MS. Population one...
<title>Abstract</title> <bold>Bovine tuberculosis (bTB) is a major disease of cattle in the UK, placing significant economic burden on taxpayer. The causative agent, </bold><italic><bold>Mycobacterium bovis</bold></italic><bold>, has wide host range, including European badger (</bold><italic><bold>Meles meles)</bold></italic><bold>. While badgers have been implicated transmission and maintenance infection areas endemic disease, their role at edge area poorly understood. Here we present data...
The SARS-CoV-2 pandemic demonstrated the importance of human coronaviruses and need to develop materials prevent spread emergent respiratory viruses. Coating surfaces with antiviral is a major interest in controlling viruses, especially high-risk or high-traffic areas. A number different coatings for have been proposed, each their own advantages disadvantages. Here we show that simple salt coating on range surfaces, including novel biomass aerogel can reduce infectivity placed onto surface....
ABSTRACT Autism spectrum disorders (ASD), Tourette syndrome (TS), and attention-deficit/hyperactivity disorder (ADHD) display strong male sex bias, due to a combination of genetic biological factors, as well selective ascertainment. While the hemizygous nature chromosome X (Chr X) in males has long been postulated key point “male vulnerability”, rare variation on this not systematically characterized large-scale whole exome sequencing studies “idiopathic” ASD, TS, ADHD. Here, we take...
Abstract Genomic instability is a hallmark of cancer resulting in widespread somatic copy number alterations. We integrated genome-scale shRNA viability screen and profiles from 179 cell lines to perform an unbiased analysis copy-number associated gene-dependency interactions. found most gene dependencies result losses genetic material rather than gains. Strikingly, the enriched class these was CYCLOPS (Copy-number alterations Yielding Cancer Liabilities Owing Partial losS) genes. Hemizygous...
The SARS-CoV-2 pandemic demonstrated the importance of human coronaviruses and need to develop materials prevent spread emergent viruses. Here we describe that simple salt coating on a range surfaces can degrade SARS-CoV-2.
To explore the application value of whole exome sequencing (WES) in diagnosis prenatal and postnatal neurodevelopmental disorders (NDDs). A total 70 patients diagnosed with NDDs who underwent WES at Medical Genetics Center Maternal Child Health Hospital Hubei Province between June 2020 July 2021 were retrospectively analyzed. Genomic DNA was extracted from peripheral blood samples amniotic fluid. WES-based copy number variant (CNV) analysis integrated into routine data pipeline. The results...
The SARS-CoV-2 pandemic demonstrated the importance of human coronaviruses and need to develop materials prevent spread emergent viruses. Here we describe that simple salt coating on a range surfaces can degrade SARS-CoV-2.