A5076386074- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Plant Pathogens and Fungal Diseases
- Plant-Microbe Interactions and Immunity
- Wastewater Treatment and Nitrogen Removal
- Nematode management and characterization studies
- Mycorrhizal Fungi and Plant Interactions
- Microbial Community Ecology and Physiology
- Agronomic Practices and Intercropping Systems
- Plant Pathogens and Resistance
- Plant Stress Responses and Tolerance
- Plant Micronutrient Interactions and Effects
- Composting and Vermicomposting Techniques
- Gut microbiota and health
- Protein Hydrolysis and Bioactive Peptides
- Plant pathogens and resistance mechanisms
- Photosynthetic Processes and Mechanisms
- Marine and coastal plant biology
- Enzyme Production and Characterization
- Coastal wetland ecosystem dynamics
- Soil Carbon and Nitrogen Dynamics
- Plant and Fungal Interactions Research
- Plant Growth Enhancement Techniques
- Enzyme Structure and Function
- Ammonia Synthesis and Nitrogen Reduction
Agricultural Research Service
2022-2025
United States Department of Agriculture
2022-2024
Dalhousie University
2016-2022
Washington State University
2006-2022
Institute of Biological Chemistry, Academia Sinica
2005-2010
All-Russian Research Institute of Agricultural Microbiology
1998-1999
One major limitation of microbial community marker gene sequencing is that it does not provide direct information on the functional composition sampled communities. Here, we present PICRUSt2 ( https://github.com/picrust/picrust2 ), which expands capabilities original PICRUSt method 1 to predict potential a based profiles. This updated and implementation includes several improvements over previous algorithm: an expanded database families reference genomes, new approach now compatible with any...
A complex network of functions and symbiotic interactions between a eukaryotic host its microbiome is the foundation ecological unit holobiont. However, little know about how non-fungal microorganisms fit in this host-microbiome interactions. In study, we employed unique wild blueberry ecosystem to evaluate plant-associated microbiota, encompassing both bacterial communities. We found that, while soil serves as for root microbiome, plant-influenced species sorting had stronger effect on...
Agricultural soils are inherently disturbed systems where organic matter additions considered to enhance microbial community structure and resilience. High-throughput sequencing of was applied receiving annual applications an alkaline stabilized biosolid (ATB), at four increasing rates over 10 years, as environmental stressor in contrast a one-time application ATB ten years prior. Bacterial more greatly influenced by relative fungi eukaryotes. Specifically, higher abundances Proteobacteria,...
<title>Abstract</title> Apple replant disease (ARD) describes a phenomenon of reduction crop productivity in the early years orchard establishment sites previously planted to apple. Currently manipulation soil microbiome by (bio)fumigation is major approach alleviate ARD. Adjustment rootstock harsh biotic factors might be an alternative combat In this study we evaluated differences structure and composition between nursery mature trees, grown Nova Scotian apple orchards. We showed that tree...
Plants and bacteria synthesize the essential human micronutrient riboflavin (vitamin B2) via same multi-step pathway. The early intermediates of this pathway are notoriously reactive may be overproduced in vivo because biosynthesis enzymes lack feedback controls. In present paper, we demonstrate disposal by COG3236 (DUF1768), a protein previously unknown function that is fused to two different plants (RIBR RibA respectively). We cheminformatic, biochemical, genetic genomic evidence show...
We examined the differences between bacterial and eukaryotic soil communities associated with natural managed habitats of wild blueberry, Vaccinium angustifolium. In total, 138 130 rhizosphere across seven blueberry fields, all established at least 30 years ago from two forest areas adjacent to some these were analyzed. analyzed correlations chemical factors structure communities, including in microbiome bulk soils, rhizospheres plants growing habitats. Characterization a broad selection...
Little is known about the ecology of microbial plastic degradation. In this study, we employed next generation amplicon sequencing to assess effect low-density polyethylene (LDPE) films on structure bacterial and fungal communities in four mature compost piles with age ranging between 2 10 years. While, Proteobacteria, Bacteroidetes, Actinobacteria fungi Ascomycota were most abundant across all facilities, our data indicated significant differences microbiomes which might be related chemical...
The Vaccinium angustifolium (wild blueberry) agricultural system involves transformation of the environment surrounding plant to intensify propagation and improve fruit yield, therefore is an advantageous model study interaction between soil microorganisms plant-host interactions. We studied this address question a trade-off microbial adaptation plant-influenced its general metabolic capabilities. found that many basic functions were similarly represented in bulk rhizosphere microbiomes...
Onion (Allium cepa) bulb rot is an important pathological problem worldwide, as it caused by diverse pathogens, bacterial and fungal. The diversity structure of microbial communities associated with apparently healthy diseased onion bulbs were analyzed. community-based approach allowed identification a number potential pathogenic microorganisms affected disease under storage environments. Enterobacteriaceae, Burkholderia, Gluconobacter, Acinetobacter, Pseudomonas, Citrobacter, Nectriaceae,...
Sinorhizobium meliloti can form a nitrogen-fixing symbiotic relationship with alfalfa after bacteria in the soil infect emerging root hairs of growing plant. To be successful at this, must able to survive between periods active plant growth, including when conditions are dry. The ability S. withstand desiccation has been known for years, but genes that contribute this phenotype have not identified. Transposon mutagenesis was used combination novel screening techniques identify four...
Abstract The maintenance of the beneficial plant microbiome to control pathogens is an emerging concept disease management, and necessitates a clear understanding these microbial communities environmental factors that affect their diversity compositional structure. As such, studies investigating economically significant cultivars within each growing region are necessary develop adequate management strategies. Here, we assessed relative impacts season, strategy, geographical location on...
Fungal pathogens pose a major threat to food production worldwide. Traditionally, chemical fungicides have been the primary means of controlling these pathogens, but many recently come under increased scrutiny due their negative effects on health humans, animals, and environment. However, use with less an environmental impact tends result in development resistance populations phytopathogenic fungi. Therefore, new environmentally friendly alternatives that provide adequate levels disease...
Epiphytic and endophytic micro-organisms associated with plants form complex communities on or in their host plant. These influence physiological traits, development, susceptibility to abiotic biotic stresses, these are theorized have evolved alongside hosts, forming a unit of selection known as the holobiont. The microbiome is highly variable can be influenced by factors, including applied exogenous agents. In this study, we compared impact chemical fungicide salicylic acid treatments...
ABSTRACT The nitrogen-fixing, symbiotic bacterium Sinorhizobium meliloti reduces molecular dinitrogen to ammonia in a specific context, supporting the nitrogen requirements of various forage legumes, including alfalfa. Determining DNA sequence S. genome was an important step plant-microbe interaction research, adding considerable information already available about this by suggesting possible functions for many >6,200 annotated open reading frames (ORFs). However, predictive power...
Sinorhizobium meliloti dctA encodes a transport protein needed for successful nitrogen-fixing symbiosis between the bacteria and alfalfa. Using toxicity of DctA substrate fluoroorotic acid as selective agent in an iterated selection procedure, four independent S. mutants were isolated that retained some ability to dicarboxylates. Two mutations located region called motif B predicted transmembrane helix has been shown other members glutamate transporter family be involved cation binding. A...
Sinorhizobium meliloti, the nitrogen-fixing bacterial symbiont of Medicago spp. and other legumes, secretes a considerable amount riboflavin. This precursor cofactors flavin mononucleotide adenine dinucleotide is bioactive molecule that has beneficial effect on plant growth. The ribBA gene S. meliloti codes for putative bifunctional enzyme with dihydroxybutanone phosphate synthase guanosine triphosphate (GTP) cyclohydrolase II activities, catalyzing initial steps riboflavin biosynthesis...
The application of bacterial inoculums for improving plant growth and production is an important component sustainable agriculture. However, the efficiency perennial crop depends on ability introduced endophytes to exert impact host-plant over extended period time. This might be evaluated by response plant-associated microbiome inoculation. In this study, we monitored effect a single strain inoculation diversity, structure, cooperation in 1-year period. An endophyte (RF67) isolated from...
The tree fruit industry in Nova Scotia, Canada, is dominated by the apple (Malus domestica) sector. However, sector faced with numerous challenges, including replant disease (ARD), which a well-known problem areas intensive cultivation. A study was performed using 16S rRNA/18S rRNA and rRNA/ITS2 amplicon sequencing to assess soil- root-associated microbiomes, respectively, from mature orchards soil microbiomes alone uncultivated soil. results indicated significant (p < 0.05) differences...
Leafy vegetables like lettuce and spinach are prone to significant post-harvest losses during handling storage. The pre-harvest treatment of crops with biostimulants offers a sustainable strategy for reducing losses. Earlier studies focused on the effect plant applied individually. In this study, we studied efficacy combined application two commonly used biostimulants: Ascophyllum nodosum extract (ANE) humic acid (HA). Interestingly, combination both improved early growth compared ANE HA...