A5051375702- Cervical Cancer and HPV Research
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Acute Myeloid Leukemia Research
- Immunotherapy and Immune Responses
- Urinary Bladder and Prostate Research
- Acute Lymphoblastic Leukemia research
- Genital Health and Disease
- Reproductive tract infections research
- Retinoids in leukemia and cellular processes
- Hepatitis B Virus Studies
- Single-cell and spatial transcriptomics
- Global Cancer Incidence and Screening
- Polyomavirus and related diseases
- Hormonal and reproductive studies
- Organ Donation and Transplantation
- Parvovirus B19 Infection Studies
- Renal Transplantation Outcomes and Treatments
- Molecular Biology Techniques and Applications
- Bioinformatics and Genomic Networks
- Cell Image Analysis Techniques
- 3D Printing in Biomedical Research
- Herpesvirus Infections and Treatments
- Colorectal Cancer Screening and Detection
- Histone Deacetylase Inhibitors Research
Guy's and St Thomas' NHS Foundation Trust
2021-2024
King's College London
2020-2023
Guy's Hospital
2022
Queen Mary University of London
2014-2021
Genomics England
2020
Cleveland Clinic
2006-2008
Virginia Commonwealth University
2003-2004
Hunter Holmes McGuire VA Medical Center
2004
B cells emerge from the bone marrow as transitional (TS) that differentiate through T1, T2, and T3 stages to become naive cells. We have identified a bifurcation of human cell maturation T1 stage forming IgMhi IgMlo developmental trajectories. T2 higher expression α4β7 integrin lower IL-4 receptor (IL4R) compared with branch are selectively recruited into gut-associated lymphoid tissue. also share transcriptomic features marginal zone (MZBs). Lineage progression MZBs via an trajectory is by...
B cells generate antibodies that are essential for immune protection, but their subgroups poorly defined. Here, we perform undirected deep profiling of in matched human lymphoid tissues from deceased transplant organ donors and blood. In addition to identifying unanticipated features tissue-based cell differentiation, resolve two subsets marginal zone (MZB) differing surface transcriptomic profiles, clonal relationships other subsets, enrichment genes the NOTCH pathway, distribution bias...
Liposomal daunorubicin and cytarabine (CPX-351) improved overall survival (OS) compared with 7+3 chemotherapy in older patients secondary acute myeloid leukemia (AML); to date, there have been no randomized studies younger patients. The high-risk cohort of the UK NCRI AML19 trial (ISRCTN78449203) CPX-351 FLAG-Ida adults newly diagnosed adverse cytogenetic AML or myelodysplastic syndromes (MDS). A total 189 were (median age, 56 years). Per clinical criteria, 49% had de novo AML, 20% 30% MDS....
Abstract Background: Human papillomavirus (HPV)-based screening is rapidly replacing cytology as the cervical modality of choice. In addition to being more sensitive than cytology, it can be done on self-collected vaginal or urine samples. This study will compare high-risk HPV positivity rates and sensitivity samples using four different collection devices a sample. Methods: A total 620 women referred for colposcopy were invited provide an initial stream sample collected with Colli-Pee...
HR HPV genotypes when assayed collectively, achieve high sensitivity but low specificity for the prediction of CIN2+. Knowledge specific in an infection may facilitate use detection routine clinical practice.To compare rate and accuracy CIN2+ between PapType test (Genera Biosystems) other commercially available assays, to examine value full genotyping.PreservCyt samples from 1099 women referred abnormal cervical cytology were used. was chosen as primary end-point CIN3+ also evaluated. A...
Background Non-attenders for cervical screening are at increased risk of cancer. Studies offering self-sampling high-risk Human Papillomavirus (HrHPV) testing have shown greater uptake than sending another invitation cytology. Objectives To explore whether would increase in a less diverse, more stable population the previous English study, which demonstrated lower response rate other studies. The primary objective was non-attenders were likely to respond postal invitation, including kit,...
Abstract Background: Human papillomavirus (HPV) testing from clinician-collected cervical and self-collected cervico-vaginal samples is more sensitive for detecting CIN2+/CIN3+ than cytology-based screening, stimulating interest in HPV urine. The objective was to determine the performance of Trovagene test detection CIN2+ urine PreservCyt samples. Methods: Women referred colposcopy at St Mary's Hospital (London, United Kingdom), following abnormal cytology, were recruited this diagnostic...
This study evaluated the Xpert HPV Assay in women attending screening general practice by comparing with two established tests, cytology and histology. A prospective aged 20–60 years Bristol, Edinburgh London using residual Preservcyt samples. Sample order was randomised between Roche cobas4800 Cepheid assays Qiagen hc2 third. 3408 cases were included primary analysis. Positivity for 19.6%, cobas 19.2% 19.9% high concordance (kappa=86.8% vs cobas, 81.55 hc2). Xpert, showed similar...
Two transport media, PreservCyt and SurePath, are widely used for cervical cytology screening. There concerns that they may perform differently HPV testing. A comparison of the performance six different tests in SurePath a referral population using two samples from each woman. The primary goal was to compare test media. Comparisons between assays viral load comparisons media were secondary aims. collected random order at same visit women with abnormal cytology. One sample placed 20 ml other...
Human papillomavirus (HPV) is the causative agent of cervical and other anogenital cancers. HPV vaccination, primarily targeted at young girls before age sexual debut, starting to demonstrate population-level declines in infection early disease associated with vaccine-incorporated genotypes. Monitoring women for vaccine-specific antibody important vaccine surveillance may be useful as an adjunct test within a screening context. We evaluated serum, dried blood spots, oral fluid potential...
<b>Introduction and Objectives:</b> We have previously reported the use of high-powered photoselective vaporization prostate (PVP) for patients in urinary retention due to benign prostatic hyperplasia (BPH). PVP is a relatively new treatment bladder outlet obstruction BPH, using laser energy vaporize obstructing tissue. This study investigates long-term follow up treated with retention. <b>Materials Methods:</b> All participants signed informed consent, were high power 80 W quasi-continuous...
Abstract B cells emerge from the bone marrow as transitional (TS) that differentiate through T1, T2 and T3 stages to become naïve cells. We have identified a bifurcation of human cell maturation T1 stage forming IgM hi lo developmental trajectories. higher expression α4β7 integrin lower IL4 receptor (IL4R) compared branch are selectively recruited into gut-associated lymphoid tissue. also share transcriptomic features with marginal zone (MZB). Lineage progression MZB via an trajectory is by...
Abstract B cells generate antibodies that are essential for immune protection. Major events driving cell responses occur in lymphoid tissues, which guide antigen acquisition and support cellular interactions, yet complexities of subsets human tissues poorly understood. Here we perform undirected, global profiling matched from deceased transplant organ donors tracked dissemination clones. In addition to identifying unanticipated features tissue-based differentiation, resolve two clonally...
High-powered photoselective vaporization of the prostate (PVP) is a relatively new addition in armamentarium against bladder outlet obstruction due to BPH. With BPH, undergoes stromal and epithelial hyperplasia, particularly transitional zone, mediated by dihydrotestosterone (DHT). This periurethral enlargement can compress prostatic urethra leading eventually urinary retention. Treatment uncomplicated symptomatic BPH has evolved from standard transurethral resection (TURP) multiple medical...
Abstract Summary Extremely large datasets are impossible or very difficult for humans to comprehend by standard mental approaches. Intuitive visualization of genetic variants in genomic sequencing data could help the review and confirmation process called automated variant calling programs. To facilitate interpretation next-generation (NGS) we developed VisVariant, a customizable tool that creates figure showing overlapping sequence information thousands individual reads including flanking...