The detection of high-risk human papillomavirus (HPV) DNA provides higher sensitivity but lower specificity than cytology for the identification high-grade cervical intraepithelial neoplasia (CIN). This study compared and several adjunctive tests CIN in a population referred to colposcopy because abnormal cytology.953 women participated study. Up seven were carried out on liquid PreservCyt sample: Hybrid Capture II (Digene), Amplicor (Roche), PreTect HPV-Proofer (NorChip), APTIMA HPV assay...

Several new assays have been developed for high-risk HPV testing of cervical samples; we compare six tests in a screening population. Residual material from liquid-based PreservCyt samples was assayed. Four (Hybrid Capture 2, Cobas, Abbott and Becton-Dickinson (BD)) measured DNA while two used RNA (APTIMA NorChip). Positivity rates ranged 13.4 to 16.3% the DNA-based with significantly lower positivity rate assay. The Gen-Probe APTIMA assay positive 10.3% women, which than all tests; NorChip...

Objective To examine the psychosocial impact of testing positive for high risk human papillomavirus (HPV) among women attending primary cervical screening. Design Cross sectional survey. Measures were taken at baseline and one week after receipt HPV cytology screening results. Setting Well women's clinic in London, UK. Population or Sample Four hundred twenty‐eight aged 20–64 years. Methods Postal questionnaire Main outcome measures Psychosocial psychosexual outcomes anxiety, distress...

High-risk human papillomavirus (HPV) DNA/RNA testing provides higher sensitivity but lower specificity than cytology for the identification of high-grade cervical intraepithelial neoplasia (CIN). Several new HPV tests are now available this purpose, and a direct comparison their properties is needed. Seven were evaluated with samples in liquid PreservCyt transport medium from 1,099 women referred colposcopy: Hybrid Capture 2 (Qiagen), Cobas (Roche), PreTect HPV-Proofer (NorChip), Aptima...

A randomised trial to ascertain whether women who do not attend for cervical screening are more likely respond the opportunity collect a self-sample human papillomavirus (HPV) testing, or further invitation screening. The study was carried out in Primary Care Trust (PCT) London between June 2009 and December 2009. In total, 3000 were randomly selected from persistent non-responders (i.e., had responded at least two invitations screening). on 1 : basis either receive an HPV self-sampling kit...

Objectives To explore self-reported cervical screening history and barriers to attendance among women who have been sexually abused identify measures improve the experience of for these women. Methods Women visiting website National Association People Abused in Childhood (NAPAC), had abused, were invited complete a survey their views experiences screening. This included closed questions on demographic characteristics attendance, open screening, opportunity submit suggestions this abused....

High‐risk human papillomavirus (hrHPV) DNA tests have excellent sensitivity for detection of cervical intraepithelial neoplasia 2 or higher (CIN2+). A drawback hrHPV screening, however, is modest specificity. Therefore, hrHPV‐positive women might need triage to reduce adverse events and costs associated with unnecessary colposcopy. We compared the performance HPV16/18 genotyping a predefined methylation test (S5) based on target regions gene EPB41L3 , viral late HPV16, HPV18, HPV31 HPV33....

<b>Objectives:</b> To assess the level and accuracy of public understanding human papillomavirus (HPV) in United Kingdom. <b>Methods:</b> Women attending a well woman clinic were asked to complete questionnaire assessing HPV awareness specific knowledge about virus. <b>Results:</b> Questionnaires completed by 1032 women, whom 30% had heard HPV. Older non-smokers, those with history candida, genital warts, or an abnormal smear result more likely have Even among who HPV, was generally poor,...

The study measured the acceptability of self-sampling for human papillomavirus (HPV) testing in context cervical cancer screening. Women carried out unsupervised, using a written instruction sheet.Participants were women attending either family planning clinic or primary care trust routine screening.Women (n = 902) HPV and then clinician did smear test. Immediately after having two tests, participants completed measure both answered questions about ease sheet willingness to use future.The...

We explored Muslim women's attitudes to self-sampling for human papillomavirus (HPV) in the context of cervical cancer screening and their responses two devices.A community centre north-east London.Following a talk given on subject HPV at centre, 28 women were recruited take part three focus group discussions. The discussion covered screening, testing. Women also asked swab kit cervico-vaginal lavage device. Discussions recorded transcribed verbatim qualitative data analysed using Framework...

Abstract Background: Human papillomavirus (HPV)-based screening is rapidly replacing cytology as the cervical modality of choice. In addition to being more sensitive than cytology, it can be done on self-collected vaginal or urine samples. This study will compare high-risk HPV positivity rates and sensitivity samples using four different collection devices a sample. Methods: A total 620 women referred for colposcopy were invited provide an initial stream sample collected with Colli-Pee...

Abstract Several studies have shown that HPV testing is substantially more sensitive than cytology for primary cervical screening. However, less data exist concerning the duration of protection afforded by a negative test compared to normal cytological outcome. Here we report long‐term findings from Hammersmith study in women aged 35 or more. Hybrid Capture II was performed on all available samples baseline screening visit. Passive surveillance subsequent results 2,982 undertaken using...

Testing for high-risk (hr) types of human papillomavirus (HPV) is highly sensitive as a screening test high-grade cervical intraepithelial neoplastic (CIN2/3) disease, the precursor cancer. However, it has relatively low specificity. Our objective was to develop prediction rule with higher specificity, using combinations and HPV DNA methylation. Exfoliated specimens from colposcopy-referral cohorts in London were analyzed methylation levels by pyrosequencing L1 L2 regions HPV16, HPV18, HPV31...

HR HPV genotypes when assayed collectively, achieve high sensitivity but low specificity for the prediction of CIN2+. Knowledge specific in an infection may facilitate use detection routine clinical practice.To compare rate and accuracy CIN2+ between PapType test (Genera Biosystems) other commercially available assays, to examine value full genotyping.PreservCyt samples from 1099 women referred abnormal cervical cytology were used. was chosen as primary end-point CIN3+ also evaluated. A...

BACKGROUND: Persistent infection %by with high risk human papillomavirus (hrHPV) types causes cervical cancer but most women who test positive are at very low of neoplasia. Strategies needed which can retain sensitivity hrHPV testin

ABSTRACT The Validation of Human Papillomavirus (HPV) Genotyping Tests (VALGENT) studies offer an opportunity to clinically validate HPV assays for use in primary screening cervical cancer and also provide a framework the comparison analytical type-specific performance. Through VALGENT, we assessed performance cartridge-based Xpert assay (Xpert HPV), which detects 14 high-risk (HR) types resolves HPV16 HPV18/45. Samples from women attending United Kingdom program enriched with cytologically...

Objective: To examine attitudes to self-sampling for human papillomavirus (HPV) testing among women from contrasting ethnic groups. Setting: Manchester, UK. Methods: Two hundred of Indian, Pakistani, African-Caribbean and white British origin were recruited social community groups participate in a questionnaire survey. The included items on intention use the test. Results: Willingness try test was high, did not foresee religious or cultural barriers self-sampling; however, large proportion...

Objectives: To compare the performance and acceptability of unsupervised self-sampling with clinician sampling for high-risk human papillomavirus (HPV) types first time in a UK screening setting. Setting: Nine hundred twenty women, from two demographically different centres, attending routine cervical smear testing Methods: Women performed an HPV self-test. Immediately afterwards, doctor or nurse took test smear. abnormality on any were offered colposcopy. Results: Twenty-one high-grade 39...

Several studies have shown that testing for high-risk human papillomavirus (HPV) types results in an improved sensitivity CIN2+, compared with cytology, although a somewhat lower specificity. We obtained follow-up results, at least one smear after participation the HART study, which HPV (HC-II) cytology as primary screening modality. With median of 6 years, 42 additional cases CIN2+ were identified; women who positive baseline more likely to develop than those negative (hazard ratio (HR)...

Background Non-attenders for cervical screening are at increased risk of cancer. Studies offering self-sampling high-risk Human Papillomavirus (HrHPV) testing have shown greater uptake than sending another invitation cytology. Objectives To explore whether would increase in a less diverse, more stable population the previous English study, which demonstrated lower response rate other studies. The primary objective was non-attenders were likely to respond postal invitation, including kit,...

Abstract Background: Human papillomavirus (HPV) testing from clinician-collected cervical and self-collected cervico-vaginal samples is more sensitive for detecting CIN2+/CIN3+ than cytology-based screening, stimulating interest in HPV urine. The objective was to determine the performance of Trovagene test detection CIN2+ urine PreservCyt samples. Methods: Women referred colposcopy at St Mary's Hospital (London, United Kingdom), following abnormal cytology, were recruited this diagnostic...

Abstract HPV DNA testing is known to be much more sensitive than cytology, but less specific. A range of and related tests in 858 women referred for colposcopy because an abnormal smear were evaluated compare the performances these tests. This article compared Abbott test other which had been previously evaluated. was a real true 14 high‐risk types. The found highly cervical intraepithelial neoplasia grade 3 or worse (CIN3+) (98.9%) with specificity 31.5%. These numbers comparable Qiagen HC2...

Objectives To explore the attitudes, views and understanding of women attending a Hindu temple in London, UK towards cervical screening, human papillomavirus (HPV) testing two HPV self-sample collection devices: Dacron swab Evalyn ® brush. Methods A mixed methods design comprising survey four focus groups was adopted. Focus group discussions were recorded transcribed verbatim explored using thematic framework analysis. Results total 185 completed surveys 23 attended groups. Of respondents...

To compare triage strategies using different human papillomavirus (HPV) consensus and genotyping tests a p16(INK4a) test.1228 women referred with borderline or single mildly dyskaryotic smear. Samples were taken at colposcopy PreservCyt. Tests included Hybrid Capture 2, Abbott RealTime PCR, BD HPV, Cobas 4800, PreTect HPV-Proofer, APTIMA p16(INK4a). Results based on the worst histology within 9 months.97/1228 (7.9%) had CIN3+ (203/1228 (17%) CIN2+). HPV testing alone 4800 sensitivity for...

This study evaluated the Xpert HPV Assay in women attending screening general practice by comparing with two established tests, cytology and histology. A prospective aged 20–60 years Bristol, Edinburgh London using residual Preservcyt samples. Sample order was randomised between Roche cobas4800 Cepheid assays Qiagen hc2 third. 3408 cases were included primary analysis. Positivity for 19.6%, cobas 19.2% 19.9% high concordance (kappa=86.8% vs cobas, 81.55 hc2). Xpert, showed similar...