A5012265087- HIV Research and Treatment
- Protein Structure and Dynamics
- HIV/AIDS drug development and treatment
- Mosquito-borne diseases and control
- Monoclonal and Polyclonal Antibodies Research
- Viral Infectious Diseases and Gene Expression in Insects
- Bee Products Chemical Analysis
- Alzheimer's disease research and treatments
- Circadian rhythm and melatonin
- Nail Diseases and Treatments
- Advanced Proteomics Techniques and Applications
- Advancements in Transdermal Drug Delivery
- Fungal Infections and Studies
- Insect and Pesticide Research
- Essential Oils and Antimicrobial Activity
- Click Chemistry and Applications
- Cellular transport and secretion
- Autophagy in Disease and Therapy
- Sirtuins and Resveratrol in Medicine
- CAR-T cell therapy research
- Dietary Effects on Health
- Enzyme Structure and Function
- Immune Cell Function and Interaction
- Biochemical and Structural Characterization
- Protein purification and stability
Mahidol University
2015-2023
Chiang Mai University
2009-2017
Université Claude Bernard Lyon 1
2009-2010
Centre National de la Recherche Scientifique
2009
Virologie et Pathologies Humaines
2009
Staphylococcus aureus is an important human pathogen, especially causing skin and soft tissue infections (SSTIs). Over the decades, caused by antibiotic-resistant strains have often become life-threatening. Consequently, exploration development of competent approaches to combat these serious circumstances are urgently required.The antibacterial activity melittin (Mel) on S. aureus, methicillin-resistant (MRSA) clinical isolates vancomycin-intermediate (VISA) was investigated minimum...
Abstract Japanese encephalitis virus (JEV), a mosquito-borne flavivirus, causes high mortality rates in humans and it is the most clinically important common cause of viral Asia. To date, there no specific treatment for JEV infection. Melatonin, neurotropic hormone, reported to be effective combating various bacterial infections. However, effects melatonin on infection have not yet been studied. The investigation tested antiviral against elucidated possible molecular mechanisms inhibition....
Background: Alzheimer’s disease (AD) is the most common cause of dementia in elderly populations. Changes expression Amyloid Precursor Protein (APP)-cleaving enzymes directly affect formation Beta (Aβ) plaques, a neuropathological hallmark AD. Objective: We used peripheral blood from AD patients to investigate genes related APP-processing [(β-site APP-cleaving enzyme 1 (BACE1), presenilin1 (PSEN1), and disintegrin metalloproteinase family 10 (ADAM10) 17 (ADAM17)] epigenetic sirtuin...
Tuberculosis (TB) therapy requires long-course multidrug regimens leading to the emergence of drug-resistant TB and increased public health burden worldwide. As treatment strategy is more challenging, seeking a potent non-antibiotic agent has been raised. Propolis serve as natural source bioactive molecules. It evidenced eliminate various microbial pathogens including Mycobacterium tuberculosis (Mtb). In this study, we fabricated niosome-based drug delivery platform for ethanolic extract...
Melittin, a major component found in bee venom, is produced by the Apis species of honey bee. In this study, effect melittin derived from florea (Mel-AF), which wild that indigenous to Thailand, was investigated against human malignant melanoma (A375) cells. Mel-AF exhibited considerable potential anti-proliferative action A375 Subsequently, cellular mechanism induced cell death terms apoptosis. As result, gene and protein expression levels, indicated activation cytochrome-c release...
Mitochondrial dysfunction is a pathological feature that manifests early in the brains of patients with Alzheimer's Disease (AD). The disruption mitochondrial dynamics contributes to morphological and functional impairments. Our previous study demonstrated expression genes involved amyloid beta generation was altered peripheral blood AD patients.The aim this further investigate relative levels dynamics, including fission fusion, mitophagy samples from compared healthy controls.The mRNA were...
This study determined the presence of anti-SARS-CoV-2 antibodies in 4964 individuals, comprising 300 coronavirus disease-19 (COVID-19) prepandemic serum samples, 142 COVID-19 patients, 2113 individuals at risk due to their occupations, 1856 sharing workplaces or communities with and 553 Thai citizens returning after spending extended periods time countries a high disease prevalence. We recruited participants between May 2020 2021, which spanned first two epidemic waves part third wave...
Cryptococcus neoformans, a life-threatening human yeast pathogen, has the ability to produce melanin, which is one of common virulence factors contributing cryptococcal pathogenesis. This factor closely associated with cell wall, specifically chitin and chitosan polysaccharides, complex structure that essential for maintaining cellular integrity. In this study, we aim investigate effects two stingless bee (SLB) propolis from Tetragonula laeviceps Tetrigona melanoleuca against wall-associated...
Secretory phospholipase B1 (PLB1) and biofilms act as microbial virulence factors play an important role in pulmonary cryptococcosis. This study aims to formulate the ethanolic extract of propolis-loaded niosomes (Nio-EEP) evaluate biological activities occurring during PLB1 production biofilm formation Cryptococcus neoformans. Some physicochemical characterizations include a mean diameter 270 nm spherical shape, zeta-potential -10.54 ± 1.37 mV, 88.13 0.01% entrapment efficiency. Nio-EEP can...
Abstract Background Cells permissive to virus can become refractory viral replication upon intracellular expression of single chain fragment variable (scFv) antibodies directed towards structural or regulatory proteins, virus-coded enzymes. For example, an intrabody derived from MH-SVM33, a monoclonal antibody against conserved C-terminal epitope the HIV-1 matrix protein (MAp17), was found exert inhibitory effect on replication. Results Two versions MH-SVM33-derived scFv were constructed in...
Taking advantage of the wide tropism baculoviruses (BVs), we constructed a recombinant BV (BV(CAR)) pseudotyped with human coxsackie B-adenovirus receptor (CAR), high-affinity attachment for adenovirus type 5 (Ad5), and used strategy piggybacking Ad5-green fluorescent protein (Ad5GFP) vector on BV(CAR) to transduce various cells refractory Ad5 infection. We found that transduction all tested, including primary cancer cell lines, was significantly improved using BV(CAR)-Ad5GFP biviral complex...
AnkGAG1D4 is an artificial ankyrin repeat protein which recognizes the capsid (CA) of human immunodeficiency virus type 1 (HIV-1) and exhibits intracellular antiviral activity on viral assembly process. Improving binding affinity would potentially enhance AnkGAG1D4-mediated activity. To augment scaffold towards its CA target, through computational predictions experimental designs. Three dimensional structure binary complex formed by docked to was used as a model for van der Waals (vdW)...
The cellular response to the recombinant NS1 protein of West Nile virus (NS1WNV) was studied using three different cell types: Vero E6 simian epithelial cells, SH-SY5Y human neuroblastoma and U-87MG astrocytoma cells. Cells were exposed two forms NS1WNV: (i) exogenous secreted form, sNS1WNV, added extracellular milieu; (ii) endogenous NS1WNV, intracellular form expressed in plasmid-transfected attachment uptake sNS1WNV varied with type only detectable Addition culture medium resulted...
Japanese encephalitis virus (JEV) infection induces uncontrolled neuronal apoptosis, leading to irreversible brain damage. However, the mechanism of JEV-induced apoptosis has not been clearly elucidated. This study aimed investigate both replication and cell during JEV in human neuroblastoma SH-SY5Y cells. As a result, kinetic productions new viral progeny were time- dose-dependent. The stimulation was dependent on multiplicity infections (MOIs) periods, particularly late period infection....
Computational analysis of protein-protein interaction provided the crucial information to increase binding affinity without a change in basic conformation. Several docking programs were used predict near-native poses complex 10 top-rankings. The universal criteria for discriminating pose are not available since there several classes recognition protein. Currently, explicit identifying ankyrin-protein complexes (APKs) have been reported yet.In this study, we established an ensemble...
Protease inhibitors (PIs) have been used to treat various types of symptoms or diseases. However, current PIs block the protease activity by targeting active site which has shown be sensitive off-target effect due crossreactivity with homologues. An alternative approach inhibiting is target substrate, specifically blocking substrate cleavage site. We propose employ synthetic biology create a E. coli as inhibitor detecting biomachine that can effectively isolate intrabodies, new generation...