A5022370161- Tracheal and airway disorders
- Streptococcal Infections and Treatments
- Medical Imaging and Pathology Studies
- Antimicrobial Resistance in Staphylococcus
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Obstructive Sleep Apnea Research
- Neuroscience of respiration and sleep
- Neonatal and Maternal Infections
- Pleural and Pulmonary Diseases
- Tuberculosis Research and Epidemiology
- Burkholderia infections and melioidosis
- Mycobacterium research and diagnosis
- Asthma and respiratory diseases
- Infectious Diseases and Tuberculosis
- Cardiac tumors and thrombi
- Head and Neck Anomalies
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Congenital Diaphragmatic Hernia Studies
- Tumors and Oncological Cases
- Salivary Gland Tumors Diagnosis and Treatment
- Voice and Speech Disorders
- Actinomycetales infections and treatment
- Infective Endocarditis Diagnosis and Management
- Eosinophilic Disorders and Syndromes
- Pediatric health and respiratory diseases
West Virginia University
2015-2024
Inje University Sanggye Paik Hospital
2006-2020
Inje University
2000-2015
Inje University Seoul Paik Hospital
1995-2012
Seoul St. Mary's Hospital
2012
University of Connecticut
2010
Park University
2006
Johannes Gutenberg University Mainz
2003
Gwangju Institute of Science and Technology
2000-2003
New England Research (United States)
2003
Clindamycin and β-lactam antibiotics have been mainstays for treating invasive group A Streptococcus (iGAS) infection, yet such regimens might be limited strains displaying MLSB phenotypes. We investigated 76 iGAS isolates from 66 patients in West Virginia, USA, during 2020-2021. performed emm typing using Centers Disease Control Prevention guidelines assessed resistance both genotypically phenotypically. Median patient age was 42 (range 23-86) years. found 76% of were simultaneously...
Apoptosis causes characteristic morphological changes in cells, including membrane blebbing, cell detachment from the extracellular matrix, and loss of cell–cell contacts. We investigated focal adhesion proteins during etoposide-induced apoptosis Rat-1 cells found that apoptosis, p130cas (Crk-associated substrate [Cas]) is cleaved by caspase-3. Sequence analysis showed Cas contains 10 DXXD consensus sites preferred identified two these (DVPD 416 G DSPD 748 G) vitro, point mutations...
Burkholderia pseudomallei and mallei, classified as category B priority pathogens, are significant human animal pathogens that highly infectious broad-spectrum antibiotic resistant. Currently, the pathogenicity mechanisms utilized by not fully understood, correct diagnosis of B. mallei infection remains a challenge due to limited detection methods. Here, we provide comprehensive analysis set 13 novel collagen-like proteins (Bucl) were identified among select agents. We infer several Bucl...
Increasing incidence of invasive group A Streptococcus (iGAS) disease has been reported in Europe and the USA over past several years. Coupled with this are observations higher rates resistance to erythromycin clindamycin.To characterize iGAS pharyngitis isolates from West Virginia (WV), a US state outside national Active Bacteria Core surveillance purview, where risk factors associated infections prevalent.Seventy-seven were collected 67 unique patients at J.W. Ruby Memorial Hospital...
The streptococcal collagen-like proteins 1 and 2 (Scl1 Scl2) are major surface adhesins that ubiquitous among group A Streptococcus (GAS). Invasive M3-type strains, however, have evolved two unique conserved features in the scl1 locus: (i) an IS1548 element insertion promoter region (ii) a nonsense mutation within coding sequence. transcript is drastically reduced GAS, contrasting with high transcription level of allele invasive M1-type GAS. This leads to lack Scl1 expression M3 strains. In...
Keratinized epidermis constitutes a powerful barrier of the mucosa and skin, effectively preventing bacterial invasion, unless it is wounded no longer protective. Wound healing involves deposition distinct extracellular matrix (ECM) proteins enriched in cellular fibronectin (cFn) isoforms containing extra domain A (EDA). The streptococcal collagen-like protein 1 (Scl1) surface adhesin group Streptococcus (GAS), which contains an N-terminal variable (V) C-terminally located domain. During...
Summary The human‐adapted pathogen group A Streptococcus (GAS) utilizes wounds as portals of entry into host tissue, wherein surface adhesins interact with the extracellular matrix, enabling bacterial colonization. streptococcal collagen‐like protein 1 (Scl1) is a major adhesin GAS that selectively binds to two fibronectin type III (FnIII) repeats within cellular fibronectin, specifically alternatively spliced extra domains and B, FnIII tenascin‐C. Binding was mediated through conserved...
Burkholderia pseudomallei is a gram-negative bacterium that the etiological agent of tropical disease melioidosis. Currently, there no licensed vaccine for melioidosis, but numerous candidates are being tested protective efficacy and characterization elicited immune response. Our lab has previously reported immunogenicity Bucl8-protein-based peptide antigen, designated L1-CRM 197 (Cross-reacting material 197). When given subcutaneously, this formulation promoted strong Th2 (IgG1) antibody...
Introduction Pancreatic ductal adenocarcinoma (PDAC) is a lethal cancer associated with an immunosuppressive environment. Neutrophil extracellular traps (NETs) were initially described in the context of infection but have more recently been implicated contributing to tolerogenic immune response PDAC. Thus, NETs are attractive target for new therapeutic strategies. Group A Streptococcus (GAS) has developed defensive strategies inhibit NETs. Methods In present work, we propose utilizing...
In the last decade, invasive group A Streptococcus (iGAS) infections have doubled in US, with equivalent increases MLSB (macrolide, lincosamide, and streptogramin B)-resistance. The emm92-type isolates carrying erm(T) gene been associated an alarming emergence of iGAS people who inject drugs or experience homelessness. Our goal was to elucidate mechanisms behind inducible (iMLSB) constitutive (cMLSB) resistance emm92 isolates. Sequence analysis identified polymorphisms regulatory region...
Extraintestinal pathogenic Escherichia coli (ExPEC) cause diseases in humans and animals, affecting organs outside the alimentary canal. In recent years, ExPEC have been reported as a of fatal pneumonia dogs, cats, horse. current report, case pleuritis is described 4-week-old tiger ( Panthera tigris) cub associated with ExPEC. The was presented sudden-onset respiratory illness died after few hours. Postmortem examination revealed an acute necrotizing pneumonia. alveolar spaces were filled...
Lymphocytic interstitial pneumonitis (LIP) is characterized by diffuse bilateral pulmonary infiltrations in both lower lobes. Pleomorphic lymphohistiocytes including mature lymphocytes, variable admixture of plasma cells and other mononuclear infiltrate within the interstitium, ranging from widened septa to confluent masses. We report a case LIP associated with Epstein-Barr virus patient SLE Sjögren's syndrome. A 50-year-old woman was admitted insidious onset progressive dyspnea for 20 days....
<b>Background</b> Postoperative acute pain can cause anxiety and decrease the quality of life in patients. Acute sternal bone after cardiac surgery persist for long time. <b>Objective</b> The aim this study is to explore relationships between degree misalignment coronary artery bypass grafting (CABG). <b>Methods</b> We retrospectively reviewed postoperative computed tomographic (CT) angiography medical records 104 patients who received CABG May 1, 2009 January 31, 2011. CT scan was...
In South Korea, chronic obstructive pulmonary disease (COPD) is one of the ten leading causes death. COPD exacerbations are significantly associated with mortality in patients. This study was conducted to investigate epidemiology specifically clinical characteristics Korean patients, exacerbation rate and risk factors exacerbations.This covers a 2-year interval. One year data collected retrospectively second prospectively obtained data.A total 1,114 subjects were enrolled study. These...
Actinomycosis is an infectious disease caused by certain Actinomyces species. are Gram-positive, non-spore forming organisms characterized obligate or facultative anaerobic rods that normally inhabit niches of the human oral cavity. Cervicofacial, abdominal, pelvic and thoracic infections not uncommon, but endobronchial actinomycosis rarely reported. Endobronchial can be misdiagnosed as unresolving pneumonia, lipoma malignancies. should included in differential diagnosis any mass.We report a...
Thymomas are one of the most common neoplasms mediastinum derived from thymic epithelium. It is that invasive thymoma invades lung, pericardium, and great vessels. Airway compression by mass effect also occurs, but direct polypoid tumor growth into airway extremely rare. Only 20 cases with endobronchial have previously been reported globally. However, there no case report endotracheal growth. Herein, we a rare in 28-year-old woman. Keywords: Thymoma; Bronchoscopy
Burkholderia pseudomallei is an infectious bacterium of clinical and biodefense concern, the causative agent melioidosis. The mortality rate can reach up to 50% affects 165,000 people per year; however, there currently no vaccine available. In this study, we examine antigen-specific immune response a formulated with antigens derived from outer membrane protein in B. pseudomallei, Bucl8. Here, employed number bioinformatic tools predict Bucl8-derived epitopes that are non-allergenic...
Bacterial efflux pumps are an important pathogenicity trait because they extrude a variety of xenobiotics. Our laboratory previously identified in silico Burkholderia collagen-like protein 8 (Bucl8) the hazardous pathogens pseudomallei and mallei . We hypothesize that Bucl8, which contains two predicted tandem outer membrane pump domains, is component putative pump. Unique to as compared other proteins, presence extended extracellular region containing (CL) domain non-collagenous C-terminus...