To investigate the protective effect of Yiqi Yangyin Huazhuo Tongluo Formula (YYHT) against high glucose-induced injury in mouse renal podocytes (MPC5 cells) and possible mechanism. Adult Wistar rats were treated with 19, 38, 76 g/kg YYHT or saline via gavage for 7 days to prepare YYHT-medicated blank sera treatment MPC5 cells cultured glucose (30 mmol/L) prior transfection a miR-21a-5p inhibitor mimic. The changes expressions mRNA levels FoxO1, PINK1, Parkin detected qRT-PCR, protein...

Abstract Esophageal cancer is one of the most common tumor in world, and morbidity rate as high 100/100 000 some parts China. Therefore, it important urgent to explore pathogenesis esophageal find new therapeutic targets for cancer. In this study, we found that a novel suppressor SPINK5 significantly reduced development cancer, closely related pathological differentiation lymph node metastasis via bioinformatics analysis tissue array. Further studies have Wnt/β‐catenin signaling pathway by...

Transducin beta-like 1X-related protein 1 (TBL1XR1) was discovered two decades ago and implicated as part of the nuclear transcription corepressor complex. Over past 20 years, emerging oncogenic function TBL1XR1 in cancer development has been discovered. Recent studies have highlighted that genetic aberrations cancers, especially hematologic tumors, are closely associated with tumorigenesis. In solid is proposed to be a promising prognostic biomarker due correlation between abnormal...

The aim of the present study was to investigate vascular normalization effect traditional Chinese medicine Astragalus membranaceus (AM) and Curcuma wenyujin (CW) on tumor-derived endothelial cells (TECs).TECs were isolated from xenografted HCC cell line HepG2 expressing red fluorescent protein (RFP). AM CW TECs proliferation measured using CCK8 assay. potential assessed a tube formation Immunocytochemistry performed assess expression angiogenic maker CD34 hypoxia-inducible factor HIF1a.The...

The aim of the study was to explore possible mechanisms that Guizhi Fuling Wan (GFW) enhances sensitivity SKOV3/DDP ovarian cancer cells and resistant xenograft tumours cisplatin. Rat medicated sera containing GFW were prepared by administering rats, primary bioactive constituents gallic acid, paeonol, paeoniflorin analysed HPLC/QqQ MS. Cell counting kit-8 analysis shown coincubation with cisplatin/paclitaxel enhanced significantly cytotoxic effect cisplatin or paclitaxel in cells. presence...

Abstract Background The well-known traditional Chinese herbal formula Guizhi Fuling Wan (GFW) was recently reported to improve the curative effects of chemotherapy for ovarian cancer with few clinical side effects. present study aimed investigate reversal mechanism sera derived from rats exposed extract (GFWE) in cisplatin-resistant human SKOV3/DDP cells; proteins examined included phosphatase and tensin homolog (PTEN) metadherin (MTDH), possible protein interaction between PTEN MTDH...

miR-155-5p is associated with autoimmune diseases. T helper 17 (Th17) cells, interleukin (IL)-17, and suppressor of cytokines signaling 1 (SOCS1) have important roles in the pathogenesis systemic sclerosis (SSc). The purpose this study was to explore role regulation IL-17 SOCS1 expression Th17 cells subsequent effect on SSc disease progression.Th17 were isolated from peripheral blood mononuclear patients healthy controls (HCs). RT-qPCR western blotting used examine patterns miR-155-5p,...

Studies have revealed the implications of cancer-associated fibroblasts (CAFs) in tumor progression, metastasis, and treatment resistance. Here, silico analyses were performed to reveal key genes pathways by which CAFs affected chemoresistance ovarian cancer.Candidate obtained from intersected differentially expressed cancer, cancer chemoresistance, CAF-related microarrays chemoresistance-related GeneCards databases. Kyoto Encyclopedia Genes Genomes enrichment analysis Gene Set Enrichment...

Diabetes mellitus is a chronic metabolic disorder characterized by high blood glucose levels, resulting from defects in insulin secretion, action, or both. Accurate and sensitive detection of concentrations crucial for the effective management diagnosis diabetes. Current techniques detection, such as enzyme-linked immunosorbent assay (ELISA) oxidase-based methods, have limitations including complex procedures, costs, insufficient selectivity. In this paper, we report development coupled...

Systemic sclerosis (SSc) is an autoimmune disease characterized by fibrosis of the skin and internal organs. So far, no Western medicine treatment can completely inhibit or reverse progress SSc, while at same time, our previous series studies have shown that SSc Wenyang Huazhuo Tongluo formula (WYHZTL), a Chinese herbal decoction, shows delightful prospect. The aim this study to further investigate mechanism anti-fibrosis WYHZTL in mouse model.The Bleomycin-induced model was treated with...

BACKGROUND: Multi-drug resistance (MDR) remains to be a major obstacle toward successful chemotherapy of NHL patients. P-glycoprotein (P-gp), classical protein associated with MDR, has been observed in peripheral blood CD56 + cells high expression an d activity. While the shown highly aggressive clinical course and chemoresistance Non-Hodgkin lymphoma (NHL). OBJECTIVE: To investigate role predicting MDR by determining P-gp function cells. METHODS: The levels MDR1 mRNA MRP1 were evaluated...

Background: Bufei decoction (BFD) is used in clinical practice to treat bronchial asthma (BA), although its molecular mechanism of action remains unclear. Objective: This study aimed explore the BFD for treating BA. Methods: Network pharmacology and docking predicted analysis results were verified using ELISA kit RT-qPCR. Results: There 58 main active components 121 potential targets from Traditional Chinese Medicine Systems Pharmacology Database Analysis Platform( TCMSP), 11 core obtained...

A family of molecular chaperone complexes called chaperonin-containing T-complex protein 1 (TCP-1) subunit genes (CCTs) aids in the folding numerous proteins. With regard to lung adenocarcinoma (LUAD), this study provided a thorough understanding diagnostic and prognostic use CCTs. The expression CCTs LUAD was evaluated by using databases including UALCAN Gene Expression Omnibus. Immunohistochemistry (IHC) conducted validate LUAD. mutation identified through cBioPortal database, while...

<title>Abstract</title> <bold>Background</bold> Ovarian cancer is one of the most lethal gynecological malignancies, primarily due to chemoresistance, which complicates effective treatment. Extrachromosomal circular DNA (eccDNA) has recently been implicated in progression and drug resistance, yet its specific contributions remain underexplored. This study investigates role WWP1-encoded eccDNA (WWP1-eccDNA) fostering resistance ovarian cells.<bold>Objective</bold> To elucidate impact...

Idiopathic pulmonary fibrosis (PF) is an irreversible and chronic inflammatory condition with limited therapeutic options a high mortality rate. We aimed to determine the possible role mechanisms of wogonin (WGN) on PF. A rat model PF was established intratracheally administrated bleomycin (BLM), followed by intravenously injecting WGN weekly body weight measurements for four weeks. Hematoxylin-eosin (H&E) Masson's trichrome staining were implemented histopathological analysis. In addition,...

Vascular damage, autoimmune abnormalities, and fibrosis are the three pathological features of systemic sclerosis (SSc).However, pulmonary vascular damage is main factor affecting progression prognosis SSc. The purpose this study was to explore molecular mechanism Wenyang Huazhuo Tongluo Formula in alleviating injury bleomycin-induced SSc mouse model.Masson staining H&E were used analyze degree infiltration leukocyte cells lung tissue ofbleomycin-induced models treated with saline (BLM...

B lymphocyte-induced maturation protein 1 (Blimp1) is a risk allele for rheumatoid arthritis (RA), but its functional mechanism in RA remains to be further explored.Flow cytometry was performed detect CD4+ T cell differentiation. ELISA used measure inflammatory factor secretion. Lentivirus mediated Blimp1 overexpression vector (LV-Blimp1) or short hairpin RNA (sh-Blimp1) were infect cells stimulated by anti-CD28 and anti-CD3 mAbs. fibroblast-like synoviocytes (FLSs) co-cultured with...

Abstract Background Chaperonin containing TCP-1 (T-complex protein 1) subunit genes (CCTs) is a family of molecular chaperone complex that assists the folding multiple proteins. However, there no comprehensive analysis CCTs in lung adenocarcinoma (LUAD). Methods UALCAN, The Human Protein Atlas and Gene Expression Omnibus (GEO) were used to evaluate mRNA expression. Immunohistochemistry was performed measure mutation detected through cBioPortal promoter methylation measured by UALCAN...