A5052870098- Physiological and biochemical adaptations
- Aquaculture Nutrition and Growth
- Ocean Acidification Effects and Responses
- Coral and Marine Ecosystems Studies
- Marine Bivalve and Aquaculture Studies
- Aquaculture disease management and microbiota
- Marine Sponges and Natural Products
- Zebrafish Biomedical Research Applications
- Fish Ecology and Management Studies
- Neurobiology and Insect Physiology Research
- Marine Biology and Ecology Research
- Crustacean biology and ecology
- Marine animal studies overview
- Ion Transport and Channel Regulation
- Marine and coastal ecosystems
- Parasite Biology and Host Interactions
- Ichthyology and Marine Biology
- Marine and coastal plant biology
- Marine and fisheries research
- Nitric Oxide and Endothelin Effects
- Cephalopods and Marine Biology
- Fish biology, ecology, and behavior
- ATP Synthase and ATPases Research
- Protist diversity and phylogeny
- Retinal Development and Disorders
Scripps Institution of Oceanography
2016-2025
University of California, San Diego
2016-2025
UC San Diego Health System
2024
Scripps (United States)
2022
Scripps Research Institute
2021
NOAA National Marine Fisheries Service Southwest Fisheries Science Center
2013
NOAA National Marine Fisheries Service
2013
Cornell University
2008-2012
Bamfield Marine Sciences Centre
2005-2009
University of Alberta
2005-2008
Symbiotic dinoflagellate algae residing inside coral tissues supply the host with majority of their energy requirements through translocation photosynthetically fixed carbon. The algae, in turn, rely on for inorganic Carbon must be concentrated as CO2 order photosynthesis to proceed, and here we show that plays an active role this process. host-derived symbiosome membrane surrounding abundantly expresses vacuolar H(+)-ATPase (VHA), which acidifies space down pH ∼ 4. Inhibition VHA results a...
The average surface pH of the ocean is dropping at a rapid rate due to dissolution anthropogenic CO 2 , raising concerns for marine life. Additionally, some coastal areas periodically experience upwelling -enriched water with reduced pH. Previous research has demonstrated acidification (OA)-induced changes in behavioural and sensory systems including olfaction, which altered function neural gamma-aminobutyric acid type A (GABA ) receptors. Here, we used camera-based tracking software system...
Scopolamine (hyoscine) is a muscarinic acetylcholine receptor antagonist that has traditionally been used to treat motion sickness in humans. However, studies investigating depressed and bipolar populations have found scopolamine also effective at reducing depression anxiety symptoms. The potential anxiety-reducing (anxiolytic) effects of could great clinical implications for humans; however, rats mice administered showed increased standard behavioural tests. This direct contrast findings...
pH homeostasis is essential for life, yet it remains unclear how animals sense their systemic acid/base (A/B) status. Soluble adenylyl cyclase (sAC) an evolutionary conserved signaling enzyme that produces the second messenger cAMP in response to bicarbonate ions (HCO 3 − ). We cloned sAC ortholog from dogfish, a shark regulates blood A/B by absorbing and secreting protons (H + ) HCO at its gills. Similar mammalian sAC, dogfish soluble (dfsAC) activated can be inhibited two structurally...
Abstract Coral reefs are facing intensifying stressors, largely due to global increases in seawater temperature and decreases pH. However, there is extensive environmental variability within coral reef ecosystems, which can impact how organisms respond trends. We deployed spatial arrays of autonomous sensors across distinct shallow habitats determine patterns spatiotemporal physicochemical parameters. Temperature pH were positively correlated over the course a day solar heating light‐driven...
Bacterial symbioses allow annelids to colonise extreme ecological niches, such as hydrothermal vents and whale falls. Yet, the genetic principles sustaining these remain unclear. Here, we show that different genomic adaptations underpin of phylogenetically related with distinct nutritional strategies. Genome compaction extensive gene losses distinguish heterotrophic symbiosis bone-eating worm Osedax frankpressi from chemoautotrophic deep-sea Vestimentifera. Osedax's endosymbionts complement...
To study the mechanisms of branchial acid-base regulation, Pacific spiny dogfish were infused intravenously for 24 h with either HCl (495+/- 79 micromol kg(-1) h(-1)) or NaHCO(3) (981+/-235 h(-1)). Infusion produced a transient reduction in blood pH. Despite continued infusion acid, pH returned to normal by 12 h. resulted new steady-state status at approximately 0.3 units higher than controls. Immunostained serial sections gill revealed presence separate vacuolar proton ATPase...
We have previously shown that continuous intravenous infusion of NaHCO3 for 24 h ( approximately 1000 micromol kg(-1) h(-1)) results in the relocation V-H+-ATPase from cytoplasm to basolateral membrane gills Pacific dogfish. To further investigate this putative base-secretive process we performed similar experiments with addition colchicine, an inhibitor cytoskeleton-dependent cellular trafficking processes. Blood pH and plasma total CO2 were significantly higher colchicines-treated, HCO3-...
Ion transport is fundamental for multiple physiological processes, including but not limited to pH regulation, calcification, and photosynthesis. Here, we investigated ion-transporting processes in tissues from the corals Acropora yongei Stylophora pistillata, representatives of complex robust clades that diverged over 250 million years ago. Antibodies against IV revealed mitochondria, an essential source ATP energetically costly ion transporters, were abundant throughout A. yongei....
Abstract The coral‐algal symbiosis is the biological engine that drives one of most spectacular structures on Earth: coral reef. Here, living microhabitats are engineered using 3D bioprinting, as biomimetic model system symbiosis. Various bioinks for encapsulation photosymbiotic microalgae ( Breviolum psygmophilum ) developed and mass transfer phenomena mimicked by bioprinting tissue skeleton microscale features. At tissue–seawater interface, polyp connective successfully replicate natural...
Acidification is a key component of digestion throughout metazoans. The gut digestive fluid many invertebrates acidified by the vesicular-type H+-ATPase (VHA). In contrast, vertebrates generate acidic fluids using gastric H+/K+-ATPase (HKA); an evolutionary innovation linked with appearance true stomach that greatly improves digestion, absorption, and immune function. Hagfishes are most basal extant vertebrates, their mechanism acidification remains unclear. Herein we report stomachless...
The upside-down jellyfish, Cassiopea spp., host their algal symbionts inside a subset of amoebocytes, phagocytic cells that also play innate immune functions akin to macrophages from vertebrate animals. Amoebocyte precursors phagocytose algae the jellyfish gut and store them intracellular compartments called symbiosomes. Subsequently, migrate mesoglea, differentiate into symbiotic roam throughout body where remain photosynthetically active supply with significant portion organic carbon...
Mammalian Soluble adenylyl cyclase (sAC, Adcy10, or Sacy) represents a source of the second messenger cAMP distinct from widely studied, G protein-regulated transmembrane cyclases. Genetic deletion through fourth coding exons in Sacy(tm1Lex)/Sacy(tm1Lex) knockout mice results male sterile phenotype. The absence any major somatic phenotype is inconsistent with variety functions identified for sAC using pharmacological inhibitors and RNA interference.We now use immunological molecular...
Isolated mitochondria-rich (MR) cells from the rainbow trout gill epithelium were subjected to intracellular pH (pH i ) imaging with pH-sensitive dye BCECF-AM. MR categorized into two distinct functional subtypes based on their ability recover an NH 4 Cl-induced acidification in absence of Na + . An apparent link between resting and -independent recovery was made. We observed a unique event that induced by extracellular addition. This further classified mixed cell population subtypes:...
Despite all the efforts and technological advances during last few decades, cellular mechanisms for branchial chloride uptake in freshwater (FW) fish are still unclear. Although a tight 1 : link with HCO-3 secretion has been established, not much is known about identity of ion-transporting proteins involved or energizing steps that allow inward transport Cl- against concentration gradient. We propose new model FW whereby combined action an apical anion exchanger, cytoplasmic carbonic...
We investigated the involvement of carbonic anhydrase (CA) in mediating V-H + -ATPase translocation into basolateral membrane gills alkalotic Squalus acanthias. Immunolabeling revealed that CA is localized same cells as -ATPase. Blood plasma from dogfish injected with acetazolamide [30 mg/kg at time ( t) = 0 and 6 h] infused NaHCO 3 for 12 h (1,000 μeq·kg −1 ·h ) had significantly higher HCO − concentration than fish were alone (28.72 ± 0.41 vs. 6.57 2.47 mmol/l, n 3), whereas blood pH was...
Intestinal HCO(3)(-) secretion and NaCl absorption are essential for counteracting dehydration in marine teleost fish. We investigated how these two processes coordinated toadfish. stimulated a luminal positive short-circuit current (I(sc)) intestine mounted Ussing chamber, bathed with the same saline solution on external internal sides of epithelium. The I(sc) increased proportionally to [HCO(3)(-)] bath up 80 mM NaHCO(3), it did not occur when NaHCO(3) was replaced Na(+)-gluconate or...