A5007505462- 3D Printing in Biomedical Research
- Additive Manufacturing and 3D Printing Technologies
- Near-Field Optical Microscopy
- Advanced Fluorescence Microscopy Techniques
- Nanofabrication and Lithography Techniques
- Nonlinear Optical Materials Studies
- Digital Holography and Microscopy
- Innovative Microfluidic and Catalytic Techniques Innovation
- Algal biology and biofuel production
- Cell Image Analysis Techniques
- Coral and Marine Ecosystems Studies
- Biocrusts and Microbial Ecology
- Orbital Angular Momentum in Optics
- Plasmonic and Surface Plasmon Research
- Photopolymerization techniques and applications
- Corneal Surgery and Treatments
- Neuroscience and Neural Engineering
- Hydrology and Watershed Management Studies
- Cephalopods and Marine Biology
- Advanced X-ray Imaging Techniques
- Tissue Engineering and Regenerative Medicine
- Photochromic and Fluorescence Chemistry
- Advanced Electron Microscopy Techniques and Applications
- Bone Tissue Engineering Materials
- Nerve injury and regeneration
Zhejiang University
2014-2024
University of Science and Technology of China
2024
Zhejiang Lab
2023-2024
University of California, San Diego
2017-2023
University of California System
2018
State Key Laboratory of Modern Optical Instruments
2014-2016
Nanyang Technological University
2016
Three-dimensional (3D) bioprinting techniques have emerged as the most popular methods to fabricate 3D-engineered tissues; however, there are challenges in simultaneously satisfying requirements of high cell density (HCD), viability, and fine fabrication resolution. In particular, resolution digital light processing-based 3D suffers with increasing bioink due scattering. We developed a novel approach mitigate this scattering-induced deterioration The inclusion iodixanol enables 10-fold...
Two-photon polymerization enables nanoscale 3D printing of hydrogels.
Abstract Glioblastoma multiforme (GBM) is the most lethal primary brain tumor characterized by high cellular and molecular heterogeneity, hypervascularization, innate drug resistance. Cellular components extracellular matrix (ECM) are two sources of heterogeneity in GBM. Here, biomimetic tri‐regional GBM models with regions, acellular ECM an endothelial region regional stiffnesses patterned corresponding to stroma, pathological or normal parenchyma, capillaries, developed. Patient‐derived...
Abstract Advances in three dimensional (3D) bioprinting have enabled the fabrication of sophisticated 3D tissue scaffolds for biological and medical applications, where high speed, throughput production well plates is a critical need. Here, we present an integrated platform based on microscale continuous optical printing, capable situ rapid complex biomedical samples multiwell plate formats subsequent culture analysis. Our bioprinter (HT-3DP) was used to showcase constructs varying spatial...
Recent advances in 3D bioprinting have transformed the tissue engineering landscape by enabling controlled placement of cells, biomaterials, and bioactive agents for biofabrication living tissues organs. However, application is limited availability cytocompatible printable biomaterials that recapitulate properties native tissues. Here, we developed an integrated projection orthogonal photoconjugation platform precision tailored microenvironments. By using a photoreactive thiol-ene gelatin...
Abstract When using light-based three-dimensional (3D) printing methods to fabricate functional micro-devices, unwanted light scattering during the process is a significant challenge achieve high-resolution fabrication. We report use of deep neural network (NN)-based machine learning (ML) technique mitigate effect, where our NN was employed study highly sophisticated relationship between input digital masks and their corresponding output 3D printed structures. Furthermore, used model an...
Abstract Digital light processing (DLP)-based three-dimensional (3D) printing technology has the advantages of speed and precision comparing with other 3D technologies like extrusion-based printing. Therefore, it is a promising biomaterial fabrication technique for tissue engineering regenerative medicine. When cell-laden biomaterials, one challenge DLP-based bioprinting scattering effect cells in bioink, therefore induce unpredictable effects on photopolymerization process. In consequence,...
Abstract The coral‐algal symbiosis is the biological engine that drives one of most spectacular structures on Earth: coral reef. Here, living microhabitats are engineered using 3D bioprinting, as biomimetic model system symbiosis. Various bioinks for encapsulation photosymbiotic microalgae ( Breviolum psygmophilum ) developed and mass transfer phenomena mimicked by bioprinting tissue skeleton microscale features. At tissue–seawater interface, polyp connective successfully replicate natural...
Limbal stem cell deficiency and corneal disorders are among the top global threats for human vision. Emerging therapies that integrate transplantation with engineered hydrogel scaffolds biological mechanical support becoming a rising trend in field. However, methods high-throughput fabrication of scaffolds, as well knowledge interaction between limbal stem/progenitor cells (LSCs) surrounding extracellular matrix (ECM) still much needed. Here, we employed digital light processing (DLP)-based...
Pterygium is an ocular surface disorder with high prevalence that can lead to vision impairment. As a pathological outgrowth of conjunctiva, pterygium involves neovascularization and chronic inflammation. Here, we developed 3D multicellular in vitro model using digital light processing (DLP)-based bioprinting platform human conjunctival stem cells (hCjSCs). A novel feeder-free culture system was adopted efficiently expanded the primary hCjSCs homogeneity, stemness differentiation potency....
The ability to noninvasively assess skeletal muscle microstructure, which predicts function and disease, would be of significant clinical value. One method that holds this promise is diffusion tensor magnetic resonance imaging (DT-MRI), sensitive the microscopic water within tissues has become ubiquitous in neuroimaging as a way assessing neuronal structure damage. However, its application assessment changes microstructure associated with injury, pathology, or age remains poorly defined,...
We propose a method for eliminating the deformations in fluorescence emission difference microscopy (FED). Due to excessive subtraction, negative values are inevitable original FED method, giving rise deformations. modulating beam generate an extended solid focal spot and hollow spot. Negative image can be avoided by using these two types of excitation spots imaging. Hence, eliminated, signal-to-noise ratio is improved. In deformation-free imaging, resolution higher than that confocal...
Polycarbonates are widely used in food packages, drink bottles, and various healthcare products such as dental sealants tooth coatings. However, bisphenol A (BPA) phosgene the production of commercial polycarbonates pose major concerns to public health safety. Here, we report a green pathway prepare BPA-free (BFPs) by thermal ring-opening polymerization photopolymerization. prepared from two cyclic carbonates different mole ratios demonstrated tunable mechanical stiffness, excellent...
This Letter introduces an iterative phase-retrieval method based on the Gerchberg–Saxton (G-S) algorithm for generating any arbitrary 3D pattern in image space, while simultaneously controlling polarization orientation at each pixel. For proof-of-principle, we generate a stereo focal spot array with distinct spot. is universal output polarization; only requirement that input should be spatially inhomogeneous. work has potential to impact coherent imaging techniques and spectroscopy.
A novel fluorescence emission difference technique is proposed for further enhancements of the lateral resolution in surface plasmon-coupled microscopy (SPCEM). In method, between image with phase modulation by using a 0-2π vortex plate (VPP) along diaphragm and original obtained from SPCEM used to estimate spatial distribution analyzed sample. By optimizing size subtractive factor, can be enhanced about 20% 33%, compared that single VPP conventional wide-field microscopy, respectively....
Abstract Two‐photon lithography (TPL) is a powerful tool for 3D nanofabrication, however, high‐throughput TPL remains challenging, especially cationic‐based photoresists. A novel photoresist named TP‐EO developed and high‐resolution nanofabrication. High‐speed fabrication achieved by using bimolecular photosensitizer‐photo acid generator (PS‐PAG) pair that can effectively solve the photosensitivity bottleneck in High‐resolution nanofabrication limiting photoacid diffusion via tuning...
We propose a method theoretically to break the diffraction limit and improve resolution in all three dimensions for fluorescence emission difference microscopy. produce two kinds of hollow focal spot by phase modulation. By incoherent superposition, these yield 3D spot. The optimal proportion is given paper. employing spot, super-resolution image can be yielded means microscopy, with enhanced both laterally axially. According computation result, size point spread function three-dimensional...
Fluorescence emission diffraction microscopy (FED) has proven to be an effective sub-diffraction-limited imaging method. In this paper, we theoretically propose a method further enhance the resolving capability of FED. Using coated mirror and only one objective lens, achieves not same axial resolution as 4Pi but also higher lateral resolution. The point spread function (PSF) our is isotropic. According calculations, full width at half-maximum (FWHM) isotropic FED's PSF 0.17λ along all three...
Spatial control of photon energy has been a central part many light-based manufacturing processes. We report direct projection printing method for ultrathin structures with nanoscale thickness by using patterned evanescent field. The field is induced total internal reflection at the interface between substrate and prepolymer solution, it phase-only spatial light modulator. structure printed on high-refractive-index glass through photopolymerization. An iterative algorithm used to calculate...