Intervertebral disc degeneration (IVDD) is one of the leading causes lower back pain and most common health problem in world. Inflammasomes, which mainly caused by NLRP3, mediated nucleus pulposus pyroptosis has been discovered to be strongly related IVDD. In addition, Duhuo Jisheng Decoction (DHJSD) anti-inflammatory regulatory effects on NLRP3 inflammasome, but molecular mechanism whether DHJSD can regulate through treat IVDD unclear. this study, we used a bioinformatics way discover...

ABSTRACT Background Intervertebral disc degeneration disease (IVDD) is a prevalent orthopedic condition that causes chronic lower back pain, imposing substantial economic burden on patients and society. Despite its high incidence, the pathophysiological mechanisms of IVDD remain incompletely understood. Objective This study aimed to identify metabolomic alterations in explore key metabolic pathways metabolites involved pathogenesis. Methods Serum samples from 20 healthy controls were...

Osteoarthritis (OA) is a widespread chronic inflammatory disease in orthopedics, and its molecular mechanisms are still poorly understood. The purpose of this work was to detect the immunological infiltration OA manner cell death utilizing bioinformatics single-cell analysis order provide guidelines for clinical therapy medicine. Ferroptosis -associated genes were sourced from ferroptosis Database, bioinformatic expression profiles chosen Gene Expression Comprehensive gene information taken...

Intervertebral disc degeneration (IVDD) is a complex age-related physiological process, with cellular senescence (CS) being primary contributing factor. However, the precise role of CS and its associated genes in IVDD remains unclear. In this study, we performed differential expression analysis on GSE124272 GSE150408 datasets from GEO database identified 53 differentially expressed senescence-related (CSRGs). We then conducted Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG)...

ABSTRACT Background Intervertebral disc degeneration (IDD) is a human aging disease related mainly to inflammation, cellular senescence, RNA/DNA methylation, and ECM. The mitogen‐activated protein kinase (MAPK) signaling pathway engaged in multiple biological functions by phosphorylating specific serine threonine residues on target proteins through phosphorylation cascade effects, but the role mechanisms of MAPK IDD are still unclear. Methods We identified 20 MAPK‐related differential genes...

Osteoarthritis (OA) is a degenerative joint disease marked by the breakdown of cartilage, where apoptosis plays key role. Although apoptosis-related genes in OA have been studied, detailed analysis PANoptosis-related and search for therapeutic drugs remains limited. We performed bioinformatics combined with single-cell RNA sequencing to examine gene expression cartilage. Key PANoptosis critical cell populations involved progression were identified. Drug prediction led selection...

Intervertebral disc degeneration (IVDD) is a common chronic disease in orthopedics, and its molecular mechanisms are still not well explained.

The purpose of this study was to examine the mechanism Duhuo Jisheng Decoction (DHJSD) in treatment intervertebral disc degeneration (IVDD).The active compounds DHJSD and their corresponding targets were obtained from TCMSP database. "Intervertebral degeneration" used as a search term DisGeNET, GeneCards, Comparative Toxicogenomics Database, MalaCards database obtain disease-related targets. Following discovery overlapping IVDD targets, enrichment analyses for Gene Ontology, Kyoto...

Abstract Recently accumulated evidence implicates a close association of vitamin D (VitD) insufficiency to the incidence and clinical manifestations COVID-19 caused by severe acute respiratory syndrome coronavirus-2 (SARS-COV-2). Populations with insufficient VitD including patients osteoporosis are more susceptible SARS-COV-2 infection worsened or developed osteoporosis. It is currently unknown, however, whether linked insufficiency. In this study, 42 common targets for on both were...

Bone nonunion is a common and serious orthopedic disorder, the occurrence of which associated with disruption dynamic balance between osteoblasts osteoclasts during bone repair. However, critical molecular mechanisms affecting this homeostasis are not well understood, it essential to investigate specific components mechanism restore promote First, we defined complex local environmental factor as "bone microenvironment" identified importance "struggle" osteoclasts, most element in determining process

Intervertebral disc degeneration (IVDD) is a prevalent orthopedic condition with lower back pain as the predominant clinical presentation that challenges treatment few therapeutic options. Duhuo Jisheng Decoction (DHJSD) has been proven effective in therapy of IVDD, but precise underlying mechanisms remain not fully elucidated. The current study was designed to test our hypothesis DHJSD may systematically correct phenotypic disruption gut microbiota and changes serum metabolome linked IVDD....

Intervertebral disc degeneration (IVDD) is an age-related orthopedic degenerative disease characterized by recurrent episodes of lower back pain, the pathogenesis which not fully understood. This study aimed to identify key biomarkers IVDD and its causes.

Intervertebral disc degeneration (IVDD) stands as a prevalent chronic orthopedic ailment, profoundly impacting patients' well-being due to incapacitating low back pain. Studies have highlighted close correlation between IVDD and the programmed cell death of nucleus pulposus (NP) cells orchestrated by interleukin-1 beta (IL-1β), tumor necrosis factor-alpha (TNF-α), caspase-3 (CASP3). Puerarin, renowned for its anti-inflammatory attributes influence on IL-1β TNF-α, emerges promising candidate...

Osteoporosis is a prevalent age-related disease that poses significant public health concern as the population continues to age. While current treatments have shown some therapeutic benefits, their long-term clinical efficacy limited by lack of stable curative effects and adverse effects. Traditional Chinese Medicine has gained attention due its positive fewer side Liuwei Dihuang Pill been found enhance bone mineral density in patients with osteoporosis rats, but underlying mechanism not yet...

Objective. Emerging evidence highlights the clinical implications of N6-methyladenosine (m6A) modification in HCC. Yet, roles m6A modulating cancer immunity and shaping tumor microenvironment (TME) are undefined hepatocellular carcinoma (HCC). Methods. Here, classification was determined for HCC through 23 modifier levels by employing consensus clustering approach. Prognosis analysis presented comparing differences survival outcomes. The ssGSEA ESTIMATE approaches were adopted evaluating...

Abstract Intervertebral disc degeneration (IDD) is a human aging disease related mainly to inflammation, cellular senescence, RNA/DNA methylation, and ECM. The mitogen-activated protein kinase (MAPK) signaling pathway engaged in multiple biological functions by phosphorylating specific serine threonine residues on target proteins through phosphorylation cascade effects, but the role mechanisms of MAPK IDD are still unclear. We obtained 20 MAPK-related differential genes analysis GSE124272...

Abstract Background Intervertebral disc degeneration (IVDD) is a common orthopedic disease in middle-aged and elderly people, the low back pain caused by it brings heavy burden on economy life of patients. The study found that apoptosis autophagy nucleus pulposus cells mediated BCL2, CASP3, CASP8, CASP9, etc. are closely related to IVDD. In addition, studies have Duhuo has effect anti-inflammation improving intervertebral degeneration, but specific molecular mechanism still unclear. Methods...