Abstract Background Oral mucositis (OM) is recognized as one of the most frequent debilitating sequelae encountered by head and neck cancer (HNC) patients treated radiotherapy. This results in severe mucosal tissue inflammation oral ulcerations that interfere with patient’s nutrition, quality life (QoL) survival. Omega-3 (ω-3) polyunsaturated fatty acids (PUFAs) have recently gained special interest dealing diseases owing to its anti-inflammatory, anti-oxidant wound healing properties. Thus,...

Abstract Introduction Eradication of residual biofilm from root canal dentine is critical for the success regenerative endodontic procedures (REPs). The aim study To evaluate influence ultrasonically activated irrigants in concentrations used REPs removal dual-species three-dimensionally printed tooth models with attached samples. Methodology Seventy-two teeth were fabricated a standardized slot apical third to ensure precise fit human specimen. Dual-species biofilms (comprising Enterococcus...

Background and aimGroup B Streptococcus (GBS) has emerged as a leading cause of illness death among neonates. The study was conducted to estimate the prevalence recto-vaginal carriage GBS pregnant women at 35–37 weeks, gestation, describe antimicrobial susceptibility profile investigate selected virulence genes by PCR.Subjects methodsTwo-hundred weeks gestation attending antenatal clinic Al-Shatby University Hospital were enrolled in study. Both vaginal rectal swabs collected from each...

To evaluate the effect of bovine colostrum (BC) on treatment children with acute diarrhea attending outpatient clinic.This double-blind randomized controlled trial was conducted 160 diarrhea; 80 cases were randomly treated BC group and received placebo (placebo group). All investigated for bacterial causes (Salmonella spp, Shigella diarrheagenic E. coli (DEC), Campylobacter spp., Vibrio cholerae) as well Rotavirus antigen in stool.After 48 h, had a significantly lower frequency vomiting,...

Diabetic foot infections (DFIs) are among the most severe complications of diabetes. The aim this study was to determine etiological pathogens DFIs in different Wagner's and IDSA/IWGDF grades, assess their antimicrobial susceptibility pattern together with molecular characterization antibiotic resistance genes.A prospective conducted on 120 DFI patients at Main Alexandria University Hospital, Egypt. aerobic anaerobic were determined using semi-quantitative culture PCR respectively. done...

Multidrug-resistant infections are an increasingly common condition particularly in critical care units. This study aimed to determine the incidence and types of resistant bacteria acquired a pediatric intensive unit (PICU) university hospital.A prospective was conducted during year 2016. All children aged below 16 years were studied for infection development pattern susceptibility various groups antibiotics.A total 264 patients admitted PICU: had community-acquired (CAI), 23...

Abstract Background To assess the antibacterial activity of multi-strain probiotics supernatants (MSP); Lactobacillus plantarum , rhamnosus and acidophilus as an intracanal medication on Enterococcus faecalis ( E. ) biofilm in a tooth model. Methods Sixty extracted human single-rooted teeth with single canals were instrumented, sterilized, inoculated . After 21 days incubation, four specimens randomly selected to validate formation by scanning electron microscope (SEM). The remaining divided...

Background: Rapid and accurate identification of healthcare associated pathogens is crucial for early diagnosis treatment infections. This study aimed to assess the performance a point-of-care multiplex polymerase chain reaction (PCR) in their antibiotic resistance genes bloodstream infections, pneumonia meningitis/encephalitis pediatric intensive care unit (PICU). Methods: A retrospective cross-sectional was conducted on patients diagnosed with infections at Alexandria University PICU,...

Abstract Background There is an increasing demand to find a potent antibacterial agent against endodontic pathogens with the least toxic effect. The study aimed evaluate activity of Lactobacilli probiotics on Enterococcus faecalis ( E. faecalis) in comparison calcium hydroxide paste. Methods involved two stages; (stage one): determination properties three strains supernatants (PS); Lactobacillus plantarum , rhamnosus acidophilus, and cocktail mix using agar well diffusion method both...

Streptococcus pneumoniae is a major cause of childhood morbidity and mortality worldwide with increasing antimicrobial resistance. 600 randomly chosen asymptomatic healthy children aged 2-60 months attending Alexandria University Children's Hospital were evaluated for prevalence nasopharyngeal (NP) carriage S. pneumoniae. Prevalence NP was 29.2% (n = 175/600) Capsular serotyping done using Quellung reaction. Vaccine covered serotypes (VST) represented 67.4% while non-vaccine (NVST) 32.6%....

Since its first identification in the early 1960s, Methicillin-Resistant Staphylococcus aureus (MRSA) has been recognized as a major human pathogen. The aim of this study was to identify prevalence MRSA Alexandria Main University Hospital and settle on simple, rapid, accurate cost-effective phenotypic test for detection from clinical specimens. One hundred S. aureus isolates, including 71 isolates, confirmed by PCR presence or absence mecA gene gold standard, were isolated patients different...

Abstract Background To assess the diagnostic performance of clinical examination and ultrasound (US) assessment knee effusion in primary osteoarthritis (KOA) patients. Furthermore, success rate for aspiration factors related to it were investigated. Methods This cross-sectional study included patients diagnosed with KOA-induced clinically or sonographically. The affected each patient was subjected US using ZAGAZIG synovitis ultrasonographic score. Patients confirmed consented prepared direct...

Abstract Background Apical surgery with standard retrograde maneuvers may be challenging in certain cases. Simplifying apical to reduce operating time and streamline manipulation is an emerging need clinical endodontics. Aim of the study The aim was compare bacterial sealing ability a calcium silicate-based sealer single cone technique combined root end resection only, as filling versus MTA retrofilling, analyze viability using confocal laser scanning microscope (CLSM). Materials methods In...

Dissemination of carbapenem resistant Enterobacteriaceae (CRE) poses a considerable threat to public health. The aim the present work was estimate prevalence metallo beta lactamases (MBL) among CRE isolated from Alexandria Main University Hospital, Egypt, evaluate performance different phenotypic methods for detection MBL, and investigate local antimicrobial sensitivity profile these isolates. Eighty were tested MBL production by Etest® MP/MPI, EDTA double disc synergy test, combined test....

Hashimoto's thyroiditis (HT) is now considered one of the most prevalent autoimmune diseases. The aim present study was to determine prevalence different types EBV antibodies in patients with HT comparison healthy controls, and detect any correlation between serological markers laboratory findings patients.This case-control conducted on 120 subjects divided into two groups: Sixty (patients group), sixty age sex matched volunteers (control group). All participants were subjected to: Thyroid...

Background: The discovery of miRNAs circulating in the peripheral blood has opened new directions research to identify non-invasive markers for diagnosis diseases. Aim: aim study was evaluate expression levels plasma (miRNA-21 & miRNA-122) Egyptian patients with chronic uncomplicated and complicated HCV. Patients Methods: This conducted on 60 Chronic HCV infected patients. were divided into three groups (20 each): HCV, cirrhosis, hepatocellular carcinoma (HCC). All subjected laboratory...

Culture-negative infective endocarditis (CNIE) is a diagnostic dilemma. The study was carried out to estimate the prevalence of CNIE among definite IE cases, describe epidemiologic and clinical characteristics patients diagnose microbial etiology using molecular serological techniques. Sixty-five cases were enrolled in prospective observational between January December 2010. tested by 16SrRNA seminested PCR for 35 blood samples, tests ten valve tissue specimens. constituted 39 (60%) cases....

The role of Escherichia coli in the pathogenesis inflammatory bowel disease (IBD) is still controversial. study aimed to investigate pathotypes and phylogenetic groups E. Egyptian patients with IBD an attempt find association between any type or group severity disease.Thirty ulcerative colitis (UC), 30 Crohn's (CD), 20 control subjects normal colonoscopy were included a cross-sectional study. isolated from stool samples by culture. Eight intestinal virulence genes coding for diarrheagenic...

The burden of pneumonia, especially that caused by respiratory viruses, is markedly high in the pediatric age group. This study aimed to assess viral agents causing severe pneumonia among mechanically ventilated patients. Nonbronchoscopic bronchoalveolar lavage was performed for patients having indicated mechanical ventilation be tested with a multiplex PCR immediate diagnosis their etiologic pathogen. Among 75 recruited, were detected 73.4% cases. Rhinovirus and syncytial virus (RSV) most...

INTRODUCTION:The fundamental aim of endodontic therapy is to completely disinfect the root canal and its three-dimensional tubular network.OBJECTIVES: This in-vitro study compared antibacterial effect intracanal irradiation with Er,Cr:YSGG laser in dry condition activated irrigation canals contaminated Enterococcus faecalis.MATERIALS AND METHODS: Twenty-six extracted human single-canaled lower bicuspids were used.Teeth instrumented autoclaved, then three specimens randomly chosen serve as...

Background & objectives: Stenotrophomonas maltophilia (S. maltophilia) emerged as an important opportunistic nosocomial multidrug resistant pathogen. This study aimed to investigate S. strains determine their virulence factors, antimicrobial resistance pattern, detect integrons and reveal genotypic relatedness. Methodology: clinical isolates were subjected susceptibility testing using the VITEK-2 compact system (BioMérieux). Biofilm production, hemolytic, protease lipase activity tested....