A5036214061- Alzheimer's disease research and treatments
- Mitochondrial Function and Pathology
- Parkinson's Disease Mechanisms and Treatments
- Metabolism and Genetic Disorders
- Genetic Neurodegenerative Diseases
- Genomics, phytochemicals, and oxidative stress
- DNA Repair Mechanisms
- Amyotrophic Lateral Sclerosis Research
- Carcinogens and Genotoxicity Assessment
- Neuroscience and Neuropharmacology Research
- Neurological disorders and treatments
- Biochemical Acid Research Studies
- Neurological diseases and metabolism
- Coenzyme Q10 studies and effects
- Tryptophan and brain disorders
- Folate and B Vitamins Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Advanced Neuroimaging Techniques and Applications
- Glutathione Transferases and Polymorphisms
- Botulinum Toxin and Related Neurological Disorders
- Neurogenetic and Muscular Disorders Research
- Mercury impact and mitigation studies
- Hemoglobinopathies and Related Disorders
- ATP Synthase and ATPases Research
- Neurological Complications and Syndromes
University of Pisa
2013-2023
Azienda Ospedaliera Universitaria Pisana
2022
Neuroscience Institute
2007
Istituto di Fisiologia Clinica
2000-2004
Universidad Cardenal Herrera CEU
2002
National Research Council
2000-2002
Medical Research Council
2000
Hammersmith Hospital
2000
Neurology, Inc
1999
<b><i><i>Background</i>: </i></b> Postmortem studies suggest excessive free radical toxicity in the substantia nigra of patients with PD. Increased lipid peroxidation and oxidative DNA damage have been reported CNS. Markers stress identified blood <b><i><i>Objective</i>: To assess presence spontaneous chromosome primary or peripheral leukocytes untreated <b><i><i>Methods</i>: Patients de novo PD (20) control subjects (16), matched for age, sex, smoking habits, underwent cytogenetic analysis...
Abstract. Objective. It is well known that free radicals contribute to endothelial dysfunction and are involved in the pathogenesis development of cardiovascular diseases, such as atherosclerosis. The aim this study was provide evidence for enhanced oxidative stress coronary artery disease (CAD). Methods. Plasma levels 8‐isoprostane (8‐ epi PGF 2 α ), marker lipid peroxidation, were measured 68 subjects (age: 60 ± years, mean SEM). Subjects included 30 healthy control 38 patients with...
To investigate the efficacy of curcumin oral supplementation (600 mg/day, Brainoil), a natural antioxidant compound, in Amyotrophic Lateral Sclerosis (ALS).Patients were randomized into two groups: Group A received placebo for 3 months, then Brainoil following B took 6 months. The evaluations conducted at basal (T0), after months double blinded or treatment (T1), and month open-label phase (T2). Clinical oxidative stress biomarkers, including protein products (AOPPs), ferric reducing ability...
Abstract The antisense oligonucleotide Nusinersen has been recently licensed to treat spinal muscular atrophy (SMA). Since SMA type 3 is characterized by variable phenotype and milder progression, biomarkers of early treatment response are urgently needed. We investigated the cerebrospinal fluid (CSF) concentration neurofilaments in patients treated with as a potential biomarker efficacy. phosphorylated heavy chain (pNfH) light (NfL) CSF was evaluated before after six months since first...
Neurodegenerative disorders (NDs) are characterized by abnormal accumulation/misfolding of specific proteins, primarily -synuclein (-syn), -amyloid1-42 (A1-42) and tau, in both brain peripheral tissues. In addition to oligomers, the role interactions α-syn with A or tau has gradually emerged. Nevertheless, despite intensive research, NDs have no accepted markers for biochemical diagnosis. this respect, Red Blood Cells (RBCs) emerging as a valid model study aging-related pathologies....
To further investigate our finding of high levels spontaneous aneuploidy in somatic cells Alzheimer’s disease (AD) patients (Migliore et al. 1997), we studied the molecular cytogenetics eight with sporadic AD and six healthy controls similar age. Cytochalasin B-blocked binucleated peripheral blood lymphocytes from unaffected were used to measure micronucleus induction or other events, such as presence malsegregation interphase nuclei (representing chromosome loss gain). Dual-color...
Somatic DNA damage has been suggested to contribute the pathogenesis of atherosclerosis. However, little is known about role oxidative in patients with coronary artery disease (CAD).In this study, we used comet assay measure (DNA strand breaks and enzyme-sensitive sites) peripheral blood lymphocytes from 13 angiographically documented CAD 11 age- sex-matched control participants.Mean values breaks, oxidized pyrimidines altered purines were significantly higher than group (11.9 +/- 1.4, 18.0...
Abstract Spinal muscular atrophy (SMA) is a neurodegenerative disorder caused by mutations in the SMN1 gene resulting reduced levels of SMN protein. Nusinersen, first antisense oligonucleotide (ASO) approved for SMA treatment, binds to SMN2 gene, paralogue , and mediates translation functional Here, we used longitudinal high-resolution mass spectrometry (MS) assess both global proteome metabolome cerebrospinal fluid (CSF) from ten type 3 patients, with aim identifying novel readouts...
Patients with Parkinson's disease (PD) in long-term levodopa therapy often complain of worsening motor symptoms the afternoon and evening. The pathophysiology this phenomenon is not known. We evaluated response to repeated doses during a 12-hour period 52 parkinsonian patients (19 de novo, 20 stable, 13 wearing-off). On day study, all received standard levodopa/carbidopa at 8:00 a.m., 12:00 noon, 4:00 p.m. Motor measurements such as tapping test, walking time, tremor score, blood samples for...
A number of evidences indicates oxidative stress as a relevant pathogenic factor in Alzheimer's disease (AD) and mild cognitive impairment (MCI). Considering its recognized major genetic risk factors AD, apolipoprotein (APO E) has been investigated several experimental settings regarding role the process reactive oxygen species (ROS) generation. The aim this work to evaluate possible relationships between APO E genotype plasma levels selected markers both AD MCI patients.APO genotypes were...
Oxidative stress involvement has been strongly hypothesized among the possible pathogenic mechanisms of motor neuron degeneration in amyotrophic lateral sclerosis (ALS). The intracellular redox balance is finely modulated by numerous complex critical for cellular functions, which nuclear factor erythroid-derived 2-like 2 (NFE2L2/Nrf2) pathways. We genotyped, a cohort ALS patients (n = 145) and healthy controls 168), three SNPs Nrf2 gene promoter: -653 A/G, -651 G/A, -617 C/A evaluated,...