A5029338354- Plant Parasitism and Resistance
- Insect-Plant Interactions and Control
- Plant-Microbe Interactions and Immunity
- Phytoplasmas and Hemiptera pathogens
- Plant Stress Responses and Tolerance
- Plant Pathogenic Bacteria Studies
- Plant and animal studies
- Lipid metabolism and biosynthesis
- Genomics and Phylogenetic Studies
- Endoplasmic Reticulum Stress and Disease
- Traditional and Medicinal Uses of Annonaceae
- Piperaceae Chemical and Biological Studies
- Chromosomal and Genetic Variations
- Insect symbiosis and bacterial influences
- Photosynthetic Processes and Mechanisms
- Genomics and Chromatin Dynamics
- Insect and Pesticide Research
- Legume Nitrogen Fixing Symbiosis
- Cocoa and Sweet Potato Agronomy
- Plant pathogens and resistance mechanisms
Leibniz Institute of Plant Biochemistry
2016-2024
Leibniz Association
2012
Phytohormones are the key metabolites participating in regulation of multiple functions plant organism. Among them, jasmonates, as well abscisic and salicylic acids responsible for triggering modulating reactions targeted against pathogens herbivores, resistance to abiotic stress (drought, UV-irradiation mechanical wounding). These factors induce dramatic changes phytohormone biosynthesis transport leading rapid local systemic responses. Understanding underlying mechanisms is principle...
Multicellular organisms rely on the movement of signaling molecules across cells, tissues, and organs to communicate among distal sites. In plants, localized leaf damage activates jasmonic acid (JA)-dependent transcriptional reprogramming in both harmed unharmed tissues. Although it has been indicated that JA species can translocate from damaged into sites, identity mobile compound(s), tissues through which they translocate, effect their relocation remain unknown. Here, we found following...
The plant pathogen Candidatus Phytoplasma mali (P. mali) is the causative agent of apple proliferation, a disease increasing importance in apple-growing areas within Europe. Despite its economic importance, little known about molecular mechanisms manifestation trees. In this study, we identified two TCP (TEOSINTE BRANCHED/CYCLOIDEA/PROLIFERATING CELL FACTOR) transcription factors Malus x domestica as binding partners P. SAP11-like effector ATP_00189. Phytohormone analyses revealed an effect...
Despite the vital roles of jasmonoyl-isoleucine (JA-Ile) in governing plant growth and environmental acclimation, it remains unclear what intracellular processes lead to its induction. Here, we provide compelling genetic evidence that mechanical osmotic regulation turgor pressure represents a key elicitor JA-Ile biosynthesis. After identifying cell wall mutant alleles KORRIGAN1 (KOR1) with elevated seedling roots, found ectopic resulted from nonautonomous signals deriving enlarged cortex...
Piperine (1-piperoyl piperidine) is responsible for the pungent perception of dried black pepper (Piper nigrum) fruits and essentially contributes to aromatic properties this spice in combination with a blend terpenoids. The final step piperine biosynthesis involves synthase (PS), which catalyzes reaction piperoyl CoA piperidine biologically active amide. Nevertheless, experimental data on cellular localization complete biosynthetic pathway are missing. Not only co-localization enzymes...
Effector proteins play an important role in the virulence of plant pathogens such as phytoplasma, which are causative agents hundreds different diseases. The hosts comprise economically relevant crops apples (Malus × domestica), can be infected by 'Candidatus Phytoplasma mali' (P. mali), a highly genetically dynamic pathogen. As result genetic and functional analyses this study, new putative P. mali effector protein was revealed. so-called "Protein Malus Expressed 2" (PME2), is expressed...
In vivo localization of proteins using fluorescence-based approaches by fusion the protein interest (POI) to a fluorescent is cost- and time-effective tool gain insights into its physiological function in plant cell. Determining proper localization, however, requires co-expression defined organelle markers (OM). Several marker sets are available but, so far, procedure successful co-transformation POI OM same cell and/or several cloning steps. We developed set vectors containing for basic...
Summary Chromatin architecture in the cells of animals and fungi influences gene expression. The molecular factors that influence higher genome plants their effects on expression remain unknown. Cohesin complexes, conserved eukaryotes, are essential structuring. Here, we investigated relevance plant-specific somatic cohesin subunit SYN4 for chromatin organisation Arabidopsis thaliana . Plants mutated were studied using HRM, Hi-C, RNA sequencing, untargeted targeted metabolomics physiological...
ABSTRACT Multicellular organisms rely upon the movement of signaling molecules across cells, tissues and organs to communicate among distal sites. In plants, herbivorous insects, necrotrophic pathogens mechanical wounding stimulate activation jasmonate (JA) pathway, which in turn triggers transcriptional changes necessary protect plants against those challenges, often at expense growth. Although previous evidence indicated that JA species can translocate from damaged into sites, identity...
ABSTRACT Despite the vital roles of jasmonoyl-isoleucine (JA-Ile) in governing plant growth and environmental acclimation, it remains unclear what intracellular processes lead to its induction. Here, we provide compelling genetic evidence that mechanical osmotic regulation turgor pressure represents a key factor eliciting JA-Ile biosynthesis. After identifying cell wall mutant alleles KORRIGAN1 ( KOR1 ) with elevated seedling roots, found ectopic resulted from non-autonomous signals deriving...
SUMMARY The unsaturation of phospholipids influences the function membranes. In Arabidopsis thaliana , oleoyl Δ12‐desaturase FAD2 converts oleic (18:1 Δ9 ) to linoleic acid (18:2 Δ9,12 and phospholipid in different cellular Despite its importance, precise localization has not been unambiguously described. As is thought modify phospholipid‐associated fatty acids at endoplasmic reticulum (ER), from where unsaturates are distributed other sites, we hypothesized that locates ER subdomains...